Genetic basis for T cell recognition of a major histocompatibility complex class II-restricted neo-self peptide

doi:10.1084/jem.182.5.1327

This Article

	Full Text (PDF, 1100K)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Cerasoli, D. M.
	Articles by Caton, A. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Cerasoli, D. M.
	Articles by Caton, A. J.


Social Bookmarking

	What's this?

ARTICLES

Genetic basis for T cell recognition of a major histocompatibility complex class II-restricted neo-self peptide

DM Cerasoli, MP Riley, FF Shih and AJ Caton
Wistar Institute, Philadelphia, Pennsylvania 19104, USA.

We have analyzed the genetic basis for T cell recognition of an endogenous major histocompatibility complex class II-restricted self peptide. Transgenic mice expressing the influenza virus PR8 hemagglutinin I-Ed-restricted determinant S1 (HA Tg mice) mediate negative selection of PR8 S1-specific T cells, but respond to immunization with a virus containing a closely related analogue, S1(K113). Sequence analysis of S1(K113)-specific T cell receptors (TCR) from nontransgenic mice revealed a dominant TCR clonotype that cross- reacts with PR8 S1. This clonotype is eliminated by negative selection in HA Tg mice; nonetheless, modified versions of this TCR that used altered junctional sequences and a novel V alpha/V beta pairing to evade negative selection by the S1 self peptide were identified. The remaining S1(K113)-specific TCRs from HA Tg mice were highly diverse; 13 of 15 S1(K113)-specific TCRs from HA Tg mice used unique V alpha/V beta pairings. Thus, tolerance to PR8 S1 as a self peptide does not limit the diversity of the T cell response to S1(K113).
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Fazilleau, N., Cabaniols, J.-P., Lemaitre, F., Motta, I., Kourilsky, P., Kanellopoulos, J. M. (2005). V{alpha} and V{beta} Public Repertoires Are Highly Conserved in Terminal Deoxynucleotidyl Transferase-Deficient Mice. J. Immunol. 174: 345-355 [Abstract] [Full Text]
Kurokawa, M., Kato, T., Masuko-Hongo, K., Ueda, S.-i., Kobata, T., Okubo, M., Nishimaki, T., Akaza, T., Yoshino, S.-i., Kasukawa, R., Nishioka, K., Yamamoto, K. (1999). Characterisation of T cell clonotypes that accumulated in multiple joints of patients with rheumatoid arthritis. Ann Rheum Dis 58: 546-553 [Abstract] [Full Text]
Gapin, L., Bravo de Alba, Y., Casrouge, A., Cabaniols, J. P., Kourilsky, P., Kanellopoulos, J. (1998). Antigen Presentation by Dendritic Cells Focuses T Cell Responses Against Immunodominant Peptides: Studies in the Hen Egg-White Lysozyme (HEL) Model1. J. Immunol. 160: 1555-1564 [Abstract] [Full Text]