Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis

doi:10.1084/jem.182.3.769

This Article

	Full Text (PDF, 2192K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Phalipon, A.
	Articles by Kraehenbuhl, J. P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Phalipon, A.
	Articles by Kraehenbuhl, J. P.


Social Bookmarking

	What's this?

ARTICLES

Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis

A Phalipon, M Kaufmann, P Michetti, JM Cavaillon, M Huerre, P Sansonetti and JP Kraehenbuhl
Unite de Pathogenie Microbienne Moleculaire, U389 Institut National de la Sante et de la Recherche Medicale, Paris, France.

To determine the role of humoral mucosal immune response in protection against shigellosis, we have obtained a monoclonal dimeric immunoglobulin A (IgA) antibody specific for Shigella flexneri serotype 5a lipopolysaccharide (mIgA) and used a murine pulmonary infection model that mimics the lesions occurring in natural intestinal infection. Adult BALB/c mice challenged with 10(7) S. flexneri organisms developed a rapid inflammatory response characterized by polymorphonuclear cell infiltration around and within the bronchi and strong systemic interleukin 6 response. Implantation of hybridoma cells in the back of mice, resulting in the development of a myeloma tumor producing mIgA in the serum and subsequently secretory mIgA in local secretions, or direct intranasal administration of these antibodies, protected the animals against subsequent intranasal challenge with S. flexneri serotype 5a. Absence of histopathological lesion and significant decrease in bacterial load of the lungs and of systemic interleukin 6 response were the three major criteria of protection. This protection was shown to be serotype-specific and dependent on local concentration of mIgA. These data demonstrate that mucosal antibodies directed against a single polysaccharidic surface epitope of Shigella can protect against the disease.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Al-Banna, N., Raghupathy, R., Albert, M. J. (2008). Correlation of Proinflammatory and Anti-Inflammatory Cytokine Levels with Histopathological Changes in an Adult Mouse Lung Model of Campylobacter jejuni Infection. CVI 15: 1780-1787 [Abstract] [Full Text]
Chamekh, M., Phalipon, A., Quertainmont, R., Salmon, I., Sansonetti, P., Allaoui, A. (2008). Delivery of Biologically Active Anti-Inflammatory Cytokines IL-10 and IL-1ra In Vivo by the Shigella Type III Secretion Apparatus. J. Immunol. 180: 4292-4298 [Abstract] [Full Text]
Al-Banna, N. A., Junaid, T. A., Mathew, T. C., Raghupathy, R., Albert, M. J. (2008). Histopathological and ultrastructural studies of a mouse lung model of Campylobacter jejuni infection. J Med Microbiol 57: 210-217 [Abstract] [Full Text]
Schroeder, G. N., Hilbi, H. (2008). Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion. Clin. Microbiol. Rev. 21: 134-156 [Abstract] [Full Text]
Kadaoui, K. A., Corthesy, B. (2007). Secretory IgA Mediates Bacterial Translocation to Dendritic Cells in Mouse Peyer's Patches with Restriction to Mucosal Compartment. J. Immunol. 179: 7751-7757 [Abstract] [Full Text]
Shim, D.-H., Suzuki, T., Chang, S.-Y., Park, S.-M., Sansonetti, P. J., Sasakawa, C., Kweon, M.-N. (2007). New Animal Model of Shigellosis in the Guinea Pig: Its Usefulness for Protective Efficacy Studies. J. Immunol. 178: 2476-2482 [Abstract] [Full Text]
Fisher, M. L., Castillo, C., Mecsas, J. (2007). Intranasal Inoculation of Mice with Yersinia pseudotuberculosis Causes a Lethal Lung Infection That Is Dependent on Yersinia Outer Proteins and PhoP. Infect. Immun. 75: 429-442 [Abstract] [Full Text]
Corthesy, B., Benureau, Y., Perrier, C., Fourgeux, C., Parez, N., Greenberg, H., Schwartz-Cornil, I. (2006). Rotavirus Anti-VP6 Secretory Immunoglobulin A Contributes to Protection via Intracellular Neutralization but Not via Immune Exclusion. J. Virol. 80: 10692-10699 [Abstract] [Full Text]
Suzuki, T., Yoshikawa, Y., Ashida, H., Iwai, H., Toyotome, T., Matsui, H., Sasakawa, C. (2006). High Vaccine Efficacy against Shigellosis of Recombinant Noninvasive Shigella Mutant That Expresses Yersinia Invasin. J. Immunol. 177: 4709-4717 [Abstract] [Full Text]
Phalipon, A., Costachel, C., Grandjean, C., Thuizat, A., Guerreiro, C., Tanguy, M., Nato, F., Vulliez-Le Normand, B., Belot, F., Wright, K., Marcel-Peyre, V., Sansonetti, P. J., Mulard, L. A. (2006). Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine. J. Immunol. 176: 1686-1694 [Abstract] [Full Text]
Clement, M.-J., Fortune, A., Phalipon, A., Marcel-Peyre, V., Simenel, C., Imberty, A., Delepierre, M., Mulard, L. A. (2006). Toward a Better Understanding of the Basis of the Molecular Mimicry of Polysaccharide Antigens by Peptides: THE EXAMPLE OF SHIGELLA FLEXNERI 5A. J. Biol. Chem. 281: 2317-2332 [Abstract] [Full Text]
Haines, G. K. III, Sayed, B. A., Rohrer, M. S., Olivier, V., Satchell, K. J. F. (2005). Role of Toll-Like Receptor 4 in the Proinflammatory Response to Vibrio cholerae O1 El Tor Strains Deficient in Production of Cholera Toxin and Accessory Toxins. Infect. Immun. 73: 6157-6164 [Abstract] [Full Text]
Le-Barillec, K., Magalhaes, J. G., Corcuff, E., Thuizat, A., Sansonetti, P. J., Phalipon, A., Di Santo, J. P. (2005). Roles for T and NK Cells in the Innate Immune Response to Shigella flexneri. J. Immunol. 175: 1735-1740 [Abstract] [Full Text]
Ohya, K., Handa, Y., Ogawa, M., Suzuki, M., Sasakawa, C. (2005). IpgB1 Is a Novel Shigella Effector Protein Involved in Bacterial Invasion of Host Cells: ITS ACTIVITY TO PROMOTE MEMBRANE RUFFLING VIA RAC1 AND CDC42 ACTIVATION. J. Biol. Chem. 280: 24022-24034 [Abstract] [Full Text]
Singer, M., Sansonetti, P. J. (2004). IL-8 Is a Key Chemokine Regulating Neutrophil Recruitment in a New Mouse Model of Shigella-Induced Colitis. J. Immunol. 173: 4197-4206 [Abstract] [Full Text]
Cersini, A., Martino, M. C., Martini, I., Rossi, G., Bernardini, M. L. (2003). Analysis of Virulence and Inflammatory Potential of Shigella flexneri Purine Biosynthesis Mutants. Infect. Immun. 71: 7002-7013 [Abstract] [Full Text]
Clement, M.-J., Imberty, A., Phalipon, A., Perez, S., Simenel, C., Mulard, L. A., Delepierre, M. (2003). Conformational Studies of the O-specific Polysaccharide of Shigella flexneri 5a and of Four Related Synthetic Pentasaccharide Fragments Using NMR and Molecular Modeling. J. Biol. Chem. 278: 47928-47936 [Abstract] [Full Text]
Casadevall, A. (2003). Antibody-Mediated Immunity against Intracellular Pathogens: Two-Dimensional Thinking Comes Full Circle. Infect. Immun. 71: 4225-4228 [Full Text]
Fullner, K. J., Boucher, J. C., Hanes, M. A., Haines, G. K. III, Meehan, B. M., Walchle, C., Sansonetti, P. J., Mekalanos, J. J. (2002). The Contribution of Accessory Toxins of Vibrio cholerae O1 El Tor to the Proinflammatory Response in a Murine Pulmonary Cholera Model. JEM 195: 1455-1462 [Abstract] [Full Text]
Rasolofo-Razanamparany, V., Cassel-Beraud, A.-M., Roux, J., Sansonetti, P. J., Phalipon, A. (2001). Predominance of Serotype-Specific Mucosal Antibody Response in Shigella flexneri-Infected Humans Living in an Area of Endemicity. Infect. Immun. 69: 5230-5234 [Abstract] [Full Text]
Adams, M. M., Allison, G. E., Verma, N. K. (2001). Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Microbiology 147: 851-860 [Abstract] [Full Text]
Bernardini, M. L., Arondel, J., Martini, I., Aidara, A., Sansonetti, P. J. (2001). Parameters Underlying Successful Protection with Live Attenuated Mutants in Experimental Shigellosis. Infect. Immun. 69: 1072-1083 [Abstract] [Full Text]
Turbyfill, K. R., Hartman, A. B., Oaks, E. V. (2000). Isolation and Characterization of a Shigella flexneri Invasin Complex Subunit Vaccine. Infect. Immun. 68: 6624-6632 [Abstract] [Full Text]
Philpott, D. J., Yamaoka, S., Israel, A., Sansonetti, P. J. (2000). Invasive Shigella flexneri Activates NF-{kappa}B Through a Lipopolysaccharide-Dependent Innate Intracellular Response and Leads to IL-8 Expression in Epithelial Cells. J. Immunol. 165: 903-914 [Abstract] [Full Text]
Jedani, K. E., Stroeher, U. H., Manning, P. A. (2000). Distribution of IS1358 and linkage to rfb-related genes in Vibrio anguillarum. Microbiology 146: 323-331 [Abstract] [Full Text]
Memet, S., Laouini, D., Epinat, J.-C., Whiteside, S. T., Goudeau, B., Philpott, D., Kayal, S., Sansonetti, P. J., Berche, P., Kanellopoulos, J., Israel, A. (1999). I{kappa}B{epsilon}-Deficient Mice: Reduction of One T Cell Precursor Subspecies and Enhanced Ig Isotype Switching and Cytokine Synthesis. J. Immunol. 163: 5994-6005 [Abstract] [Full Text]
Arondel, J., Singer, M., Matsukawa, A., Zychlinsky, A., Sansonetti, P. J. (1999). Increased Interleukin-1 (IL-1) and Imbalance between IL-1 and IL-1 Receptor Antagonist during Acute Inflammation in Experimental Shigellosis. Infect. Immun. 67: 6056-6066 [Abstract] [Full Text]
Crottet, P., Peitsch, M. C., Servis, C., Corthesy, B. (1999). Covalent Homodimers of Murine Secretory Component Induced by Epitope Substitution Unravel the Capacity of the Polymeric Ig Receptor to Dimerize Noncovalently in the Absence of IgA Ligand. J. Biol. Chem. 274: 31445-31455 [Abstract] [Full Text]
Way, S. S., Borczuk, A. C., Goldberg, M. B. (1999). Thymic Independence of Adaptive Immunity to the Intracellular Pathogen Shigella flexneri Serotype 2a. Infect. Immun. 67: 3970-3979 [Abstract] [Full Text]
Forestier, C., Moreno, E., Meresse, S., Phalipon, A., Olive, D., Sansonetti, P., Gorvel, J.-P. (1999). Interaction of Brucella abortus Lipopolysaccharide with Major Histocompatibility Complex Class II Molecules in B Lymphocytes. Infect. Immun. 67: 4048-4054 [Abstract] [Full Text]
Adhikari, P., Allison, G., Whittle, B., Verma, N. K. (1999). Serotype 1a O-Antigen Modification: Molecular Characterization of the Genes Involved and Their Novel Organization in the Shigella flexneri Chromosome. J. Bacteriol. 181: 4711-4718 [Abstract] [Full Text]
Launay, P., Grossetete, B., Arcos-Fajardo, M., Gaudin, E., Torres, S. P., Beaudoin, L., Patey-Mariaud de Serre, N., Lehuen, A., Monteiro, R. C. (1999). Fc{alpha} Receptor (CD89) Mediates the Development of Immunoglobulin A (IgA) Nephropathy (Berger's Disease): Evidence for Pathogenic Soluble Receptor-IgA Complexes in Patients and CD89 Transgenic Mice. JEM 191: 1999-2010 [Abstract] [Full Text]
Beatty, W. L., Meresse, S., Gounon, P., Davoust, J., Mounier, J., Sansonetti, P. J., Gorvel, J.-P. (1999). Trafficking of Shigella Lipopolysaccharide in Polarized Intestinal Epithelial Cells. JCB 145: 689-698 [Abstract] [Full Text]
Way, S. S., Borczuk, A. C., Goldberg, M. B. (1999). Adaptive Immune Response to Shigella flexneri 2a cydC in Immunocompetent Mice and Mice Lacking Immunoglobulin A. Infect. Immun. 67: 2001-2004 [Abstract] [Full Text]
Crottet, P., Corthesy, B. (1998). Secretory Component Delays the Conversion of Secretory IgA into Antigen-Binding Competent F(ab')2: A Possible Implication for Mucosal Defense. J. Immunol. 161: 5445-5453 [Abstract] [Full Text]
Jensen, V. B., Harty, J. T., Jones, B. D. (1998). Interactions of the Invasive Pathogens Salmonella typhimurium, Listeria monocytogenes, and Shigella flexneri with M Cells and Murine Peyer's Patches. Infect. Immun. 66: 3758-3766 [Abstract] [Full Text]
Turbyfill, K. R., Mertz, J. A., Mallett, C. P., Oaks, E. V. (1998). Identification of Epitope and Surface-Exposed Domains of Shigella flexneri Invasion Plasmid Antigen D (IpaD). Infect. Immun. 66: 1999-2006 [Abstract] [Full Text]
Marcotte, H., Lavoie, M. C. (1998). Oral Microbial Ecology and the Role of Salivary Immunoglobulin A. Microbiol. Mol. Biol. Rev. 62: 71-109 [Abstract] [Full Text]