Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-1

doi:10.1084/jem.182.2.501

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Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-1

M Saifuddin, CJ Parker, ME Peeples, MK Gorny, S Zolla-Pazner, M Ghassemi, IA Rooney, JP Atkinson and GT Spear
Department of Immunology/Microbiology, Rush University, Chicago, Illinois 60612, USA.

This study investigates whether cell-derived glycosylphosphatidylinositol-linked complement control proteins CD55 and CD59 can be incorporated into HIV-1 virions and contribute to complement resistance. Virus was prepared by transfection of cell lines with pNL4-3, and primary isolates of HIV-1 were derived from patients' PBMCs. Virus was tested for sensitivity to complement-mediated virolysis in the presence of anti-gp160 antibody. Viral preparations from JY33 cells, which lack CD55 and CD59, were highly sensitive to complement. HIV-1 preparations from H9 and U937 cells, which express low levels of CD55 and CD59, had intermediate to high sensitivity while other cell line-derived viruses and primary isolates of HIV-1 were resistant to complement-mediated virolysis. Although the primary isolates were not lysed, they activated complement as measured by binding to a complement receptor positive cell line. While the primary isolates were resistant to lysis in the presence of HIV-specific antibody, antibody to CD59 induced lysis. Likewise, antibody to CD55 and CD59 induced lysis of cell line-derived virus. Western blot analysis of purified virus showed bands corresponding to CD55 and CD59. Phosphatidylinositol-specific phospholipase C treatment of either cell line-derived or primary isolates of HIV-1 increased sensitivity to complement while incubation of sensitive virus with purified CD55 and CD59 increased resistance to complement. These results show that CD55 and CD59 are incorporated into HIV-1 particles and function to protect virions from complement-mediated destruction, and they are the first report of host cell proteins functioning in protection of HIV-1 from immune effector mechanisms.
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Walport, M. J. (2001). Complement- First of Two Parts. NEJM 344: 1058-1066 [Full Text]
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Lawn, S. D., Roberts, B. D., Griffin, G. E., Folks, T. M., Butera, S. T. (2000). Cellular Compartments of Human Immunodeficiency Virus Type 1 Replication In Vivo: Determination by Presence of Virion-Associated Host Proteins and Impact of Opportunistic Infection. J. Virol. 74: 139-145 [Abstract] [Full Text]
Cavacini, L. A., Peterson, J. E., Nappi, E., Duval, M., Goldstein, R., Mayer, K., Posner, M. R. (1999). Minimal Incidence of Serum Antibodies Reactive with Intact Primary Isolate Virions in Human Immunodeficiency Virus Type 1-Infected Individuals. J. Virol. 73: 9638-9641 [Abstract] [Full Text]
DePolo, N. J., Harkleroad, C. E., Bodner, M., Watt, A. T., Anderson, C. G., Greengard, J. S., Murthy, K. K., Dubensky, T. W. Jr., Jolly, D. J. (1999). The Resistance of Retroviral Vectors Produced from Human Cells to Serum Inactivation In Vivo and In Vitro Is Primate Species Dependent. J. Virol. 73: 6708-6714 [Abstract] [Full Text]
Mazzetti, P., Giannecchini, S., Del Mauro, D., Matteucci, D., Portincasa, P., Merico, A., Chezzi, C., Bendinelli, M. (1999). AIDS Vaccination Studies Using an Ex Vivo Feline Immunodeficiency Virus Model: Detailed Analysis of the Humoral Immune Response to a Protective Vaccine. J. Virol. 73: 1-10 [Abstract] [Full Text]
Ikeda, F., Haraguchi, Y., Jinno, A., Iino, Y., Morishita, Y., Shiraki, H., Hoshino, H. (1998). Human Complement Component C1q Inhibits the Infectivity of Cell-Free HTLV-I. J. Immunol. 161: 5712-5719 [Abstract] [Full Text]
Paquette, J.-S., Fortin, J.-F., Blanchard, L., Tremblay, M. J. (1998). Level of ICAM-1 Surface Expression on Virus Producer Cells Influences both the Amount of Virion-Bound Host ICAM-1 and Human Immunodeficiency Virus Type 1�Infectivity. J. Virol. 72: 9329-9336 [Abstract] [Full Text]
Vanderplasschen, A., Mathew, E., Hollinshead, M., Sim, R. B., Smith, G. L. (1998). Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope. Proc. Natl. Acad. Sci. USA 95: 7544-7549 [Abstract] [Full Text]
Wyatt, R., Sodroski, J. (1998). The HIV-1 Envelope Glycoproteins: Fusogens, Antigens, and Immunogens. Science 280: 1884-1888 [Abstract] [Full Text]
Fortin, J.-F., Cantin, R., Tremblay, M. J. (1998). T Cells Expressing Activated LFA-1 Are More Susceptible to Infection with Human Immunodeficiency Virus Type 1�Particles Bearing Host-Encoded ICAM-1. J. Virol. 72: 2105-2112 [Abstract] [Full Text]
Cantin, R., Fortin, J.-F., Lamontagne, G., Tremblay, M. (1997). The Acquisition of Host-Derived Major Histocompatibility Complex Class II Glycoproteins by Human Immunodeficiency Virus Type 1�Accelerates the Process of Virus Entry and Infection in Human T-Lymphoid Cells. Blood 90: 1091-1100 [Abstract] [Full Text]
Bounou, S., Dumais, N., Tremblay, M. J. (2001). Attachment of Human Immunodeficiency Virus-1 (HIV-1) Particles Bearing Host-encoded B7-2 Proteins Leads to Nuclear Factor-kappa B- and Nuclear Factor of Activated T Cells-dependent Activation of HIV-1 Long Terminal Repeat Transcription. J. Biol. Chem. 276: 6359-6369 [Abstract] [Full Text]