Constitutive and allergen-induced expression of eotaxin mRNA in the guinea pig lung

doi:10.1084/jem.181.3.1211

This Article

	Full Text (PDF, 696K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rothenberg, M. E.
	Articles by Leder, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rothenberg, M. E.
	Articles by Leder, P.


Social Bookmarking

	What's this?

ARTICLES

Constitutive and allergen-induced expression of eotaxin mRNA in the guinea pig lung

ME Rothenberg, AD Luster, CM Lilly, JM Drazen and P Leder
Howard Hughes Medical Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.

Eotaxin is a member of the C-C family of chemokines and is related during antigen challenge in a guinea pig model of allergic airway inflammation (asthma). Consistent with its putative role in eosinophilic inflammation, eotaxin induces the selective infiltration of eosinophils when injected into the lung and skin. Using a guinea pig lung cDNA library, we have cloned full-length eotaxin cDNA. The cDNA encodes a protein of 96 amino acids, including a putative 23-amino acid hydrophobic leader sequence, followed by 73 amino acids composing the mature active eotaxin protein. The protein-coding region of this cDNA is 73, 71, 50, and 48% identical in nucleic acid sequence to those of human macrophage chemoattractant protein (MCP) 3, MCP-1, macrophage inflammatory protein (MIP) 1 alpha, and RANTES, respectively. Analysis of genomic DNA suggested that there is a single eotaxin gene in guinea pig which is apparently conserved in mice. High constitutive levels of eotaxin mRNA expression were observed in the lung, while the intestines, stomach, spleen, liver, heart, thymus, testes, and kidney expressed lower levels. To determine if eotaxin mRNA levels are elevated during allergen-induced eosinophilic airway inflammation, ovalbumin (OVA)-sensitized guinea pigs were challenged with aerosolized antigen. Compared with the lungs from saline-challenged animals, eotaxin mRNA levels increased sixfold within 3 h and returned to baseline by 6 h. Thus, eotaxin mRNA levels are increased in response to allergen challenge during the late phase response. The identification of constitutive eotaxin mRNA expression in multiple tissues suggests that in addition to regulating airway eosinophilia, eotaxin is likely to be involved in eosinophil recruitment into other tissues as well as in baseline tissue homing.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Yan, B M, Shaffer, E A (2009). Primary eosinophilic disorders of the gastrointestinal tract. Gut 58: 721-732 [Abstract] [Full Text]
Levina, V., Nolen, B. M., Marrangoni, A. M., Cheng, P., Marks, J. R., Szczepanski, M. J., Szajnik, M. E., Gorelik, E., Lokshin, A. E. (2009). Role of Eotaxin-1 Signaling in Ovarian Cancer. Clin. Cancer Res. 15: 2647-2656 [Abstract] [Full Text]
Forbes, E. E., Groschwitz, K., Abonia, J. P., Brandt, E. B., Cohen, E., Blanchard, C., Ahrens, R., Seidu, L., McKenzie, A., Strait, R., Finkelman, F. D., Foster, P. S., Matthaei, K. I., Rothenberg, M. E., Hogan, S. P. (2008). IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity. JEM 205: 897-913 [Abstract] [Full Text]
Haley, K. J., Sunday, M. E., Porrata, Y., Kelley, C., Twomey, A., Shahsafaei, A., Galper, B., Sonna, L. A., Lilly, C. M. (2008). Ontogeny of the eotaxins in human lung. Am. J. Physiol. Lung Cell. Mol. Physiol. 294: L214-L224 [Abstract] [Full Text]
Pope, S. M., Zimmermann, N., Stringer, K. F., Karow, M. L., Rothenberg, M. E. (2005). The Eotaxin Chemokines and CCR3 Are Fundamental Regulators of Allergen-Induced Pulmonary Eosinophilia. J. Immunol. 175: 5341-5350 [Abstract] [Full Text]
Vermaelen, K., Pauwels, R. (2005). Pulmonary Dendritic Cells. Am. J. Respir. Crit. Care Med. 172: 530-551 [Abstract] [Full Text]
Kodali, R. B., Kim, W. J.H., Galaria, I. I., Miller, C., Schecter, A. D., Lira, S. A., Taubman, M. B. (2004). CCL11 (Eotaxin) Induces CCR3-Dependent Smooth Muscle Cell Migration. Arterioscler. Thromb. Vasc. Bio. 24: 1211-1216 [Abstract] [Full Text]
Bramley, A. M., Khan, M. A., Manson, H. E., Hegele, R. G. (2003). Development of Respiratory Syncytial Virus "Bronchiolitis" in Guinea Pigs Does Not Reflect an Allergic Predisposition in the Host. Chest 124: 671-681 [Abstract] [Full Text]
Takizawa, H., Abe, S., Okazaki, H., Kohyama, T., Sugawara, I., Saito, Y., Ohtoshi, T., Kawasaki, S., Desaki, M., Nakahara, K., Yamamoto, K., Matsushima, K., Tanaka, M., Sagai, M., Kudoh, S. (2003). Diesel exhaust particles upregulate eotaxin gene expression in human bronchial epithelial cells via nuclear factor-kappa B-dependent pathway. Am. J. Physiol. Lung Cell. Mol. Physiol. 284: L1055-L1062 [Abstract] [Full Text]
Price, K. S., Friend, D. S., Mellor, E. A., De Jesus, N., Watts, G. F. M., Boyce, J. A. (2003). CC Chemokine Receptor 3 Mobilizes to the Surface of Human Mast Cells and Potentiates Immunoglobulin E-Dependent Generation of Interleukin 13. Am. J. Respir. Cell Mol. Bio. 28: 420-427 [Abstract] [Full Text]
Beaulieu, S., Robbiani, D. F., Du, X., Rodrigues, E., Ignatius, R., Wei, Y., Ponath, P., Young, J. W., Pope, M., Steinman, R. M., Mojsov, S. (2002). Expression of a Functional Eotaxin (CC Chemokine Ligand 11) Receptor CCR3 by Human Dendritic Cells. J. Immunol. 169: 2925-2936 [Abstract] [Full Text]
Stubbs, V. E. L., Schratl, P., Hartnell, A., Williams, T. J., Peskar, B. A., Heinemann, A., Sabroe, I. (2002). Indomethacin Causes Prostaglandin D2-like and Eotaxin-like Selective Responses in Eosinophils and Basophils. J. Biol. Chem. 277: 26012-26020 [Abstract] [Full Text]
Cheng, S. S., Lukacs, N. W., Kunkel, S. L. (2002). Eotaxin/CCL11 Suppresses IL-8/CXCL8 Secretion from Human Dermal Microvascular Endothelial Cells. J. Immunol. 168: 2887-2894 [Abstract] [Full Text]
Morse, B., Sypek, J. P., Donaldson, D. D., Haley, K. J., Lilly, C. M. (2002). Effects of IL-13 on airway responses in the guinea pig. Am. J. Physiol. Lung Cell. Mol. Physiol. 282: L44-L49 [Abstract] [Full Text]
Li, Y., Martin, L. D., Minnicozzi, M., Greenfeder, S., Fine, J., Pettersen, C. A., Chorley, B., Adler, K. B. (2001). Enhanced Expression of Mucin Genes in a Guinea Pig Model of Allergic Asthma. Am. J. Respir. Cell Mol. Bio. 25: 644-651 [Abstract] [Full Text]
Gouon-Evans, V., Pollard, J. W. (2001). Eotaxin Is Required for Eosinophil Homing into the Stroma of the Pubertal and Cycling Uterus. Endocrinology 142: 4515-4521 [Abstract] [Full Text]
Berkman, N., Ohnona, S., Chung, F. K., Breuer, R. (2001). Eotaxin-3 but Not Eotaxin Gene Expression Is Upregulated in Asthmatics 24 Hours after Allergen Challenge. Am. J. Respir. Cell Mol. Bio. 24: 682-687 [Abstract] [Full Text]
Matsukura, S., Stellato, C., Georas, S. N., Casolaro, V., Plitt, J. R., Miura, K., Kurosawa, S., Schindler, U., Schleimer, R. P. (2001). Interleukin-13 Upregulates Eotaxin Expression in Airway Epithelial Cells by a STAT6-Dependent Mechanism. Am. J. Respir. Cell Mol. Bio. 24: 755-761 [Abstract] [Full Text]
LILLY, C. M., NAKAMURA, H., BELOSTOTSKY, O. I., HALEY, K. J., GARCIA-ZEPEDA, E. A., LUSTER, A. D., ISRAEL, E. (2001). Eotaxin Expression after Segmental Allergen Challenge in Subjects with Atopic Asthma. Am. J. Respir. Crit. Care Med. 163: 1669-1675 [Abstract] [Full Text]
Stahl, J. L., Cook, E. B., Pariza, M. A., Cook, M. E., Graziano, F. M. (2001). Effect of L-Tryptophan Supplementation on Eosinophils and Eotaxin in Guinea Pigs. Exp. Biol. Med. 226: 177-184 [Abstract] [Full Text]
Falcone, F. H., Haas, H., Gibbs, B. F. (2000). The human basophil: a new appreciation of its role in immune responses. Blood 96: 4028-4038 [Full Text]
Zimmermann, N., Hogan, S. P., Mishra, A., Brandt, E. B., Bodette, T. R., Pope, S. M., Finkelman, F. D., Rothenberg, M. E. (2000). Murine Eotaxin-2: A Constitutive Eosinophil Chemokine Induced by Allergen Challenge and IL-4 Overexpression. J. Immunol. 165: 5839-5846 [Abstract] [Full Text]
Shahabuddin, S., Ponath, P., Schleimer, R. P. (2000). Migration of Eosinophils Across Endothelial Cell Monolayers: Interactions Among IL-5, Endothelial-Activating Cytokines, and C-C Chemokines. J. Immunol. 164: 3847-3854 [Abstract] [Full Text]
Matsukura, S., Stellato, C., Plitt, J. R., Bickel, C., Miura, K., Georas, S. N., Casolaro, V., Schleimer, R. P. (1999). Activation of Eotaxin Gene Transcription by NF-{kappa}B and STAT6 in Human Airway Epithelial Cells. J. Immunol. 163: 6876-6883 [Abstract] [Full Text]
Guo, R.-F., Ward, P. A., Jordan, J. A., Huber-Lang, M., Warner, R. L., Shi, M. M. (1999). Eotaxin Expression in Sephadex-Induced Lung Injury in Rats. Am. J. Pathol. 155: 2001-2008 [Abstract] [Full Text]
Stellato, C., Matsukura, S., Fal, A., White, J., Beck, L. A., Proud, D., Schleimer, R. P. (1999). Differential Regulation of Epithelial-Derived C-C Chemokine Expression by IL-4 and the Glucocorticoid Budesonide. J. Immunol. 163: 5624-5632 [Abstract] [Full Text]
Ying, S., Robinson, D. S., Meng, Q., Barata, L. T., McEuen, A. R., Buckley, M. G., Walls, A. F., Askenase, P. W., Kay, A. B. (1999). C-C Chemokines in Allergen-Induced Late-Phase Cutaneous Responses in Atopic Subjects: Association of Eotaxin with Early 6-Hour Eosinophils, and of Eotaxin-2 and Monocyte Chemoattractant Protein-4 with the Later 24-Hour Tissue Eosinophilia, and Relationship to Basophils and Other C-C Chemokines (Monocyte Chemoattractant Protein-3 and RANTES). J. Immunol. 163: 3976-3984 [Abstract] [Full Text]
Rothenberg, M. E. (1999). Eotaxin . An Essential Mediator of Eosinophil Trafficking into Mucosal Tissues. Am. J. Respir. Cell Mol. Bio. 21: 291-295 [Full Text]
HISADA, T., SALMON, M., NASUHARA, Y., CHUNG, K. F. (1999). Cysteinyl-leukotrienes Partly Mediate Eotaxin-induced Bronchial Hyperresponsiveness and Eosinophilia in IL-5 Transgenic Mice. Am. J. Respir. Crit. Care Med. 160: 571-575 [Abstract] [Full Text]
Gonzalo, J.-A., Pan, Y., Lloyd, C. M., Jia, G.-Q., Yu, G., Dussault, B., Powers, C. A., Proudfoot, A. E. I., Coyle, A. J., Gearing, D., Gutierrez-Ramos, J.-C. (1999). Mouse Monocyte-Derived Chemokine Is Involved in Airway Hyperreactivity and Lung Inflammation. J. Immunol. 163: 403-411 [Abstract] [Full Text]
Franz-Bacon, K., Dairaghi, D. J., Boehme, S. A., Sullivan, S. K., Schall, T. J., Conlon, P. J., Taylor, N., Bacon, K. B. (1999). Human Thymocytes Express CCR-3 and Are Activated by Eotaxin. Blood 93: 3233-3240 [Abstract] [Full Text]
Hisada, T., Hellewell, P. G., Teixeira, M. M., Malm, M. G.�K., Salmon, M., Huang, T.-J., Fan Chung, K. (1999). alpha 4 Integrin-Dependent Eotaxin Induction of Bronchial Hyperresponsiveness and Eosinophil Migration in Interleukin-5 Transgenic Mice. Am. J. Respir. Cell Mol. Bio. 20: 992-1000 [Abstract] [Full Text]
Watson, M. L., White, A.-M., Campbell, E. M., Smith, A. W., Uddin, J., Yoshimura, T., Westwick, J. (1999). Anti-Inflammatory Actions of Interleukin-13 . Suppression of Tumor Necrosis Factor-alpha and Antigen-Induced Leukocyte Accumulation in the Guinea Pig Lung. Am. J. Respir. Cell Mol. Bio. 20: 1007-1012 [Abstract] [Full Text]
Rossi, D. L., Hurst, S. D., Xu, Y., Wang, W., Menon, S., Coffman, R. L., Zlotnik, A. (1999). Lungkine, a Novel CXC Chemokine, Specifically Expressed by Lung Bronchoepithelial Cells. J. Immunol. 162: 5490-5497 [Abstract] [Full Text]
Struyf, S., Proost, P., Schols, D., De Clercq, E., Opdenakker, G., Lenaerts, J.-P., Detheux, M., Parmentier, M., De Meester, I., Scharpe, S., Van Damme, J. (1999). CD26/Dipeptidyl-Peptidase IV Down-Regulates the Eosinophil Chemotactic Potency, But Not the Anti-HIV Activity of Human Eotaxin by Affecting Its Interaction with CC Chemokine Receptor 3. J. Immunol. 162: 4903-4909 [Abstract] [Full Text]
Jinquan, T., Quan, S., Feili, G., Larsen, C. G., Thestrup-Pedersen, K. (1999). Eotaxin Activates T Cells to Chemotaxis and Adhesion Only if Induced to Express CCR3 by IL-2 Together with IL-4. J. Immunol. 162: 4285-4292 [Abstract] [Full Text]
MacLean, J. A., Sauty, A., Luster, A. D., Drazen, J. M., De Sanctis, G. T. (1999). Antigen-Induced Airway Hyperresponsiveness, Pulmonary Eosinophilia, and Chemokine Expression in B Cell-Deficient Mice. Am. J. Respir. Cell Mol. Bio. 20: 379-387 [Abstract] [Full Text]
Li, L., Xia, Y., Nguyen, A., Lai, Y. H., Feng, L., Mosmann, T. R., Lo, D. (1999). Effects of Th2 Cytokines on Chemokine Expression in the Lung: IL-13 Potently Induces Eotaxin Expression by Airway Epithelial Cells. J. Immunol. 162: 2477-2487 [Abstract] [Full Text]
KRAFT, M., MARTIN, R.�J., WILSON, S., DJUKANOVIC, R., HOLGATE, S.�T. (1999). Lymphocyte and Eosinophil Influx into Alveolar Tissue in Nocturnal Asthma. Am. J. Respir. Crit. Care Med. 159: 228-234 [Abstract] [Full Text]
Sabroe, I., Conroy, D. M., Gerard, N. P., Li, Y., Collins, P. D., Post, T. W., Jose, P. J., Williams, T. J., Gerard, C. J., Ponath, P. D. (1998). Cloning and Characterization of the Guinea Pig Eosinophil Eotaxin Receptor, C-C Chemokine Receptor-3: Blockade Using a Monoclonal Antibody In Vivo. J. Immunol. 161: 6139-6147 [Abstract] [Full Text]
Yang, Y., Loy, J., Ryseck, R.-P., Carrasco, D., Bravo, R. (1998). Antigen-Induced Eosinophilic Lung Inflammation Develops in Mice Deficient in Chemokine Eotaxin. Blood 92: 3912-3923 [Abstract] [Full Text]
Huang, W.-W., Garcia-Zepeda, E. A., Sauty, A., Oettgen, H. C., Rothenberg, M. E., Luster, A. D. (1998). Molecular and Biological Characterization of the Murine Leukotriene B4 Receptor Expressed on Eosinophils. JEM 188: 1063-1074 [Abstract] [Full Text]
Matthews, A. N., Friend, D. S., Zimmermann, N., Sarafi, M. N., Luster, A. D., Pearlman, E., Wert, S. E., Rothenberg, M. E. (1998). Eotaxin is required for the baseline level of tissue eosinophils. Proc. Natl. Acad. Sci. USA 95: 6273-6278 [Abstract] [Full Text]
Sanz, M.-J., Ponath, P. D., Mackay, C. R., Newman, W., Miyasaka, M., Tamatani, T., Flanagan, B. F., Lobb, R. R., Williams, T. J., Nourshargh, S., Jose, P. J. (1998). Human Eotaxin Induces {alpha}4 and {beta}2 Integrin-Dependent Eosinophil Accumulation in Rat Skin In Vivo: Delayed Generation of Eotaxin in Response to IL-4. J. Immunol. 160: 3569-3576 [Abstract] [Full Text]
Palframan, R. T., Collins, P. D., Williams, T. J., Rankin, S. M. (1998). Eotaxin Induces a Rapid Release of Eosinophils and Their Progenitors From the Bone Marrow. Blood 91: 2240-2248 [Abstract] [Full Text]
Peled, A., Gonzalo, J. A., Lloyd, C., Gutierrez-Ramos, J.-C. (1998). The Chemotactic Cytokine Eotaxin Acts as a Granulocyte-Macrophage Colony-Stimulating Factor During Lung Inflammation. Blood 91: 1909-1916 [Abstract] [Full Text]
Ishii, Y., Shirato, M., Nomura, A., Sakamoto, T., Uchida, Y., Ohtsuka, M., Sagai, M., Hasegawa, S. (1998). Cloning of rat eotaxin: ozone inhalation increases mRNA and protein expression in lungs of Brown Norway rats. Am. J. Physiol. Lung Cell. Mol. Physiol. 274: L171-L176 [Abstract] [Full Text]
Minshall, E. M., Cameron, L., Lavigne, F., Leung, D. Y.�M., Hamilos, D., Garcia-Zepada, E. A., Rothenberg, M., Luster, A. D., Hamid, Q. (1997). Eotaxin mRNA and Protein Expression in Chronic Sinusitis and Allergen-induced Nasal Responses in Seasonal Allergic Rhinitis. Am. J. Respir. Cell Mol. Bio. 17: 683-690 [Abstract] [Full Text]
Humbles, A. A., Conroy, D. M., Marleau, S., Rankin, S. M., Palframan, R. T., Proudfoot, A. E.I., Wells, T. N.C., Li, D., Jeffery, P. K., Griffiths-Johnson, D. A., Williams, T. J., Jose, P. J. (1997). Kinetics of Eotaxin Generation and Its Relationship to Eosinophil Accumulation in Allergic Airways Disease: Analysis in a Guinea Pig Model In Vivo. JEM 186: 601-612 [Abstract] [Full Text]
Rollins, B. J. (1997). Chemokines. Blood 90: 909-928 [Full Text]
Nibbs, R. J.�B., Wylie, S. M., Pragnell, I. B., Graham, G. J. (1997). Cloning and Characterization of a Novel Murine beta �Chemokine Receptor, D6. COMPARISON TO THREE OTHER RELATED MACROPHAGE INFLAMMATORY PROTEIN-1alpha RECEPTORS, CCR-1, CCR-3, AND CCR-5. J. Biol. Chem. 272: 12495-12504 [Abstract] [Full Text]
Rothenberg, M. E., MacLean, J. A., Pearlman, E., Luster, A. D., Leder, P. (1997). Targeted Disruption of the Chemokine Eotaxin Partially Reduces Antigen-induced Tissue Eosinophilia. JEM 185: 785-790 [Abstract] [Full Text]
HOGAN, S.�P., KOSKINEN, A., MATTHAEI, K.�I., YOUNG, I.�G., FOSTER, P.�S. (1997). Interleukin-5-producing CD4+ T Cells Play a Pivotal Role in Aeroallergen-induced Eosinophilia, Bronchial Hyperreactivity, and Lung Damage in Mice. Am. J. Respir. Crit. Care Med. 157: 210-218 [Abstract] [Full Text]
Huber, M. A., Denk, A., Peter, R. U., Weber, L., Kraut, N., Wirth, T. (2002). The IKK-2/Ikappa Balpha /NF-kappa B Pathway Plays a Key Role in the Regulation of CCR3 and eotaxin-1 in Fibroblasts. A CRITICAL LINK TO DERMATITIS IN Ikappa Balpha -DEFICIENT MICE. J. Biol. Chem. 277: 1268-1275 [Abstract] [Full Text]