Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties

doi:10.1084/jem.180.6.2287

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Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties

J Brennan, D Mager, W Jefferies and F Takei
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.

The murine Ly-49 antigen belongs to a family of type II transmembrane molecules containing lectin-like domains. The original member of this family, Ly-49A, has been demonstrated to be expressed by a subpopulation of natural killer (NK) cells, bind certain class I major histocompatibility complexes (MHC), and act as a negative regulator of lytic activity. The expression patterns and functional activities of the other Ly-49s, however, is unknown. We extended the study of this family by isolating cDNAs encoding two new Ly-49 molecules. The reactivity of these and previously identified Ly-49 molecules with NK antibodies was tested in a COS cell expression system. YE1/32 and YE1/48 bound Ly-49A specifically, and 5E6 reacted only with Ly-49C. Three-color flow cytometric analysis demonstrated Ly-49A and Ly-49C expression defines complex, but distinct subsets within NK1.1+ cells. Some NK1.1-CD3+ as well as NK1.1-CD3- cells expressing Ly-49A or C were also detected. Analysis of MHC congenic strains of mice demonstrated that YE1/32+ and YE1/48+ NK cells are not deleted, as has been shown with the Ly-49A mAb A1. Furthermore, COS cells transfected with Ly-49A bound H-2d and H-2k cell lines, whereas Ly-49C transfectants bound H- 2d, H-2k, H-2b, and H-2s. The antibodies 5E6 and 34-1-2S (anti-class I MHC) inhibited the binding of Ly-49C to an H-2s cell line. These results imply that the NK cell antigens Ly-49A and C bind to different repertoires of class I MHC molecules.
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Saleh, A., Makrigiannis, A. P., Hodge, D. L., Anderson, S. K. (2002). Identification of a Novel Ly49 Promoter That Is Active in Bone Marrow and Fetal Thymus. J. Immunol. 168: 5163-5169 [Abstract] [Full Text]
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Brawand, P., Lemonnier, F. A., MacDonald, H. R., Cerottini, J.-C., Held, W. (2000). Transgenic Expression of Ly49A on T Cells Impairs a Specific Antitumor Response. J. Immunol. 165: 1871-1876 [Abstract] [Full Text]
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Korten, S., Wilk, E., Gessner, J. E., Meyer, D., Schmidt, R. E. (1999). Altered Donor and Recipient Ly49+ NK Cell Subsets in Allogeneic H-2d -> H-2b and H-2b -> H-2d Bone Marrow Chimeras. J. Immunol. 163: 5896-5905 [Abstract] [Full Text]
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Williams, N. S., Klem, J., Puzanov, I. J., Sivakumar, P. V., Bennett, M., Kumar, V. (1999). Differentiation of NK1.1+, Ly49+ NK Cells from flt3+ Multipotent Marrow Progenitor Cells. J. Immunol. 163: 2648-2656 [Abstract] [Full Text]
Lian, R. H., Li, Y., Kubota, S., Mager, D. L., Takei, F. (1999). Recognition of Class I MHC by NK Receptor Ly-49C: Identification of Critical Residues. J. Immunol. 162: 7271-7276 [Abstract] [Full Text]
George, T. C., Mason, L. H., Ortaldo, J. R., Kumar, V., Bennett, M. (1999). Positive Recognition of MHC Class I Molecules by the Ly49D Receptor of Murine NK Cells. J. Immunol. 162: 2035-2043 [Abstract] [Full Text]
Vos, Q., Snapper, C. M., Mond, J. J. (1999). Heterogeneity in the ability of cytotoxic murine NK cell clones to enhance Ig secretion in vitro. Int Immunol 11: 159-168 [Abstract] [Full Text]
Nakamura, M. C., Linnemeyer, P. A., Niemi, E. C., Mason, L. H., Ortaldo, J. R., Ryan, J. C., Seaman, W. E. (1999). Mouse Ly-49D Recognizes H-2Dd and Activates Natural Killer Cell Cytotoxicity. JEM 189: 493-500 [Abstract] [Full Text]
Idris, A. H., Iizuka, K., Smith, H. R.C., Scalzo, A. A., Yokoyama, W. M. (1998). Genetic Control Of Natural Killing and In Vivo Tumor Elimination by the Chok Locus. JEM 188: 2243-2256 [Abstract] [Full Text]
Seo, N., Tokura, Y., Furukawa, F., Takigawa, M. (1998). Down-Regulation of Tumoricidal NK and NK T Cell Activities by MHC Kb Molecules Expressed on Th2-Type {gamma}{delta} T and {alpha}{beta} T Cells Coinfiltrating in Early B16 Melanoma Lesions. J. Immunol. 161: 4138-4145 [Abstract] [Full Text]
Lian, R. H., Freeman, J. D., Mager, D. L., Takei, F. (1998). Role of Conserved Glycosylation Site Unique to Murine Class I MHC in Recognition by Ly-49 NK Cell Receptor. J. Immunol. 161: 2301-2306 [Abstract] [Full Text]
Smith, K. M., Wu, J., Bakker, A. B. H., Phillips, J. H., Lanier, L. L. (1998). Cutting Edge: Ly-49D and Ly-49H Associate with Mouse DAP12 and Form Activating Receptors. J. Immunol. 161: 7-10 [Abstract] [Full Text]
Sundback, J., Nakamura, M. C., Waldenstrom, M., Niemi, E. C., Seaman, W. E., Ryan, J. C., Karre, K. (1998). The {alpha}2 Domain of H-2Dd Restricts the Allelic Specificity of the Murine NK Cell Inhibitory Receptor Ly-49A. J. Immunol. 160: 5971-5978 [Abstract] [Full Text]
Ho, E. L., Heusel, J. W., Brown, M. G., Matsumoto, K., Scalzo, A. A., Yokoyama, W. M. (1998). Murine Nkg2d and Cd94 are clustered within the natural killer complex and are expressed independently in natural killer cells. Proc. Natl. Acad. Sci. USA 95: 6320-6325 [Abstract] [Full Text]
Chang, C. S., Kane, K. P. (1998). Evidence for Sulfate Modification of H-2Dd on N-Linked Carbohydrate(s): Possible Involvement in Ly-49A Interaction. J. Immunol. 160: 4367-4374 [Abstract] [Full Text]
Lee, N., Llano, M., Carretero, M., Ishitani, A., Navarro, F., Lopez-Botet, M., Geraghty, D. E. (1998). HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc. Natl. Acad. Sci. USA 95: 5199-5204 [Abstract] [Full Text]
Dorfman, J. R., Raulet, D. H. (1998). Acquisition of Ly49 Receptor Expression by Developing Natural Killer Cells. JEM 187: 609-618 [Abstract] [Full Text]
Ortaldo, J. R., Winkler-Pickett, R., Mason, A. T., Mason, L. H. (1998). The Ly-49 Family: Regulation of Cytotoxicity and Cytokine Production in Murine CD3+ Cells. J. Immunol. 160: 1158-1165 [Abstract] [Full Text]
Held, W., Raulet, D. H. (1997). Ly49A Transgenic Mice Provide Evidence for a Major Histocompatibility Complex-dependent Education Process in Natural Killer Cell Development. JEM 185: 2079-2088 [Abstract] [Full Text]
Nakamura, M. C., Niemi, E. C., Fisher, M. J., Shultz, L. D., Seaman, W. E., Ryan, J. C. (1997). Mouse Ly-49A Interrupts Early Signaling Events in Natural Killer Cell Cytotoxicity and Functionally Associates with the SHP-1 Tyrosine Phosphatase. JEM 185: 673-684 [Abstract] [Full Text]
Iizuka, K., Kaneko, H., Yamada, T., Kimura, H., Kokai, Y., Fujimoto, J. (1997). Host F1 Mice Pretreated With Granulocyte Colony-Stimulating Factor Accept Parental Bone Marrow Grafts in Hybrid Resistance System. Blood 89: 1446-1451 [Abstract] [Full Text]
Mason, L.H., Anderson, S.K., Yokoyama, W.M., Smith, H.R.C., Winkler-Pickett, R., Ortaldo, J.R. (1996). The Ly-49D Receptor Activates Murine Natural Killer Cells. JEM 184: 2119-2128 [Abstract] [Full Text]
Brennan, J., Lemieux, S., Freeman, J. D., Mager, D. L., Takei, F. (1996). Heterogeneity Among Ly-49C Natural Killer (NK) Cells: Characterization of Highly Related Receptors with Differing Functions and Expression Patterns. JEM 184: 2085-2090 [Abstract] [Full Text]
Brennan, J., Takei, F., Wong, S., Mager, D. L. (1995). Carbohydrate Recognition by a Natural Killer Cell Receptor, Ly-49C. J. Biol. Chem. 270: 9691-9694 [Abstract] [Full Text]
Wang, J., Whitman, M. C., Natarajan, K., Tormo, J., Mariuzza, R. A., Margulies, D. H. (2002). Binding of the Natural Killer Cell Inhibitory Receptor Ly49A to Its Major Histocompatibility Complex Class I Ligand. CRUCIAL CONTACTS INCLUDE BOTH H-2Dd AND beta 2-MICROGLOBULIN. J. Biol. Chem. 277: 1433-1442 [Abstract] [Full Text]