NH2-terminal sequence of macrophage-expressed natural resistance- associated macrophage protein (Nramp) encodes a proline/serine-rich putative Src homology 3-binding domain

doi:10.1084/jem.179.5.1683

This Article

	Full Text (PDF, 607K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Barton, C. H.
	Articles by Blackwell, J. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Barton, C. H.
	Articles by Blackwell, J. M.


Social Bookmarking

	What's this?

ARTICLES

NH2-terminal sequence of macrophage-expressed natural resistance- associated macrophage protein (Nramp) encodes a proline/serine-rich putative Src homology 3-binding domain

CH Barton, JK White, TI Roach and JM Blackwell
Department of Medicine, University of Cambridge Clinical School, Addenbrooke's Hospital, United Kingdom.

The Lsh/Ity/Bcg locus on mouse chromosome 1 regulates macrophage (m phi) priming/activation for antimicrobial activity against intracellular pathogens. A candidate Bcg gene, designated natural resistance-associated m phi protein (Nramp), recently isolated from a pre-B cell cDNA library encodes a polytopic integral membrane protein with structural features common to prokaryotic and eukaryotic transporters. In the present study, an activated m phi cDNA library yielded new Nramp clones that differ in the 5' region from the published pre-B cell-derived clone sequence, resulting in addition of 64 amino acids at the NH2 terminus of the predicted protein. This new domain is rich in proline, serine, and basic amino acids, and includes three protein kinase C phosphorylation sites and a putative Src homology 3 binding domain. RNAs containing this domain are the only form found in the m phi. Hence, the protein encoded by this RNA is the candidate molecule mediating natural resistance to intra-m phi pathogens.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Techau, M. E., Valdez-Taubas, J., Popoff, J.-F., Francis, R., Seaman, M., Blackwell, J. M. (2007). Evolution of Differences in Transport Function in Slc11a Family Members. J. Biol. Chem. 282: 35646-35656 [Abstract] [Full Text]
Stober, C. B., Brode, S., White, J. K., Popoff, J.-F., Blackwell, J. M. (2007). Slc11a1, Formerly Nramp1, Is Expressed in Dendritic Cells and Influences Major Histocompatibility Complex Class II Expression and Antigen-Presenting Cell Function. Infect. Immun. 75: 5059-5067 [Abstract] [Full Text]
Folwell, J. L., Barton, C. H., Shepherd, D. (2006). Immunolocalisation of the D. melanogaster Nramp homologue Malvolio to gut and Malpighian tubules provides evidence that Malvolio and Nramp2 are orthologous. J. Exp. Biol. 209: 1988-1995 [Abstract] [Full Text]
Wyllie, S., Seu, P., Gao, F. Q., Gros, P., Goss, J. A. (2002). Disruption of the Nramp1 (also known as Slc11a1) gene in Kupffer cells attenuates early-phase, warm ischemia-reperfusion injury in the mouse liver. J. Leukoc. Biol. 72: 885-897 [Abstract] [Full Text]
Bowen, H., Biggs, T. E., Phillips, E., Baker, S. T., Perry, V. H., Mann, D. A., Barton, C. H. (2002). c-Myc Represses and Miz-1 Activates the Murine Natural Resistance-associated Protein 1 Promoter. J. Biol. Chem. 277: 34997-35006 [Abstract] [Full Text]
Buschman, E., Skamene, E. (2001). From Bcg/Lsh/Ity to Nramp1: Three Decades of Search and Research. Drug Metab. Dispos. 29: 471-473 [Full Text]
Kuhn, D. E., Lafuse, W. P., Zwilling, B. S. (2001). Iron transport into Mycobacterium avium-containing phagosomes from an Nramp1Gly169-transfected RAW264.7 macrophage cell line. J. Leukoc. Biol. 69: 43-49 [Abstract] [Full Text]
Wojciechowski, W., DeSanctis, J., Skamene, E., Radzioch, D. (1999). Attenuation of MHC Class II Expression in Macrophages Infected with Mycobacterium bovis Bacillus Calmette-Guerin Involves Class II Transactivator and Depends on the Nramp1 Gene. J. Immunol. 163: 2688-2696 [Abstract] [Full Text]
Rojas, M., Olivier, M., Gros, P., Barrera, L. F., Garcia, L. F. (1999). TNF-{alpha} and IL-10 Modulate the Induction of Apoptosis by Virulent Mycobacterium tuberculosis in Murine Macrophages. J. Immunol. 162: 6122-6131 [Abstract] [Full Text]
Zwilling, B. S., Kuhn, D. E., Wikoff, L., Brown, D., Lafuse, W. (1999). Role of Iron in Nramp1-Mediated Inhibition of Mycobacterial Growth. Infect. Immun. 67: 1386-1392 [Abstract] [Full Text]
Soo, S.-S., Villarreal-Ramos, B., Khan, C. M.�A., Hormaeche, C. E., Blackwell, J. M. (1998). Genetic Control of Immune Response to Recombinant Antigens Carried by an Attenuated Salmonella typhimurium Vaccine Strain: Nramp1 Influences T-Helper Subset Responses and Protection against Leishmanial Challenge. Infect. Immun. 66: 1910-1917 [Abstract] [Full Text]
Searle, S, Bright, N., Roach, T., Atkinson, P., Barton, C., Meloen, R., Blackwell, J. (1998). Localisation of Nramp1 in macrophages: modulation with activation and infection. J. Cell Sci. 111: 2855-2866 [Abstract]
Gruenheid, S., Pinner, E., Desjardins, M., Gros, P. (1997). Natural Resistance to Infection with Intracellular Pathogens: The Nramp1 Protein Is Recruited to the Membrane of the Phagosome. JEM 185: 717-730 [Abstract] [Full Text]
Feng, J, Li, Y, Hashad, M, Schurr, E, Gros, P, Adams, L G, Templeton, J W (1996). Bovine natural resistance associated macrophage protein 1 (Nramp1) gene.. Genome Res 6: 956-964 [Abstract]