p56lck interacts via its src homology 2 domain with the ZAP-70 kinase

doi:10.1084/jem.179.4.1163

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p56lck interacts via its src homology 2 domain with the ZAP-70 kinase

P Duplay, M Thome, F Herve and O Acuto
Department of Immunology, Institut Pasteur, Paris, France.

p56lck, a member of the src family of protein tyrosine kinases, is an essential component in T cell receptor (TCR) signal transduction. p56lck contains a src homology 2 (SH2) domain found in a number of proteins involved in intracellular signaling. SH2 domains have been implicated in protein-protein interactions by binding to sequences in target proteins containing phosphorylated tyrosine. Using an in vitro assay, we have studied specific binding of tyrosine-phosphorylated proteins to a recombinant p56lck SH2 domain. In nonactivated Jurkat cells, two tyrosine-phosphorylated proteins were detected. Stimulation with anti-CD3 monoclonal antibodies induced the binding of seven additional tyrosine-phosphorylated proteins to the SH2 domain of p56lck. We have identified the zeta-associated tyrosine kinase, ZAP-70, as one of these proteins. Evidence suggests that binding of ZAP-70 to p56lck SH2 is direct and not mediated by zeta. The significance of this interaction was further investigated in vivo. p56lck could be coprecipitated with the zeta/ZAP-70 complex and conversely, ZAP-70 was detected in p56lck immunoprecipitates of activated Jurkat cells. The physical association of p56lck and ZAP-70 during activation supports the recently proposed functional cooperation of these two tyrosine kinases in TCR signaling.
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Stachlewitz, R. F., Hart, M. A., Bettencourt, B., Kebede, T., Schwartz, A., Ratnofsky, S. E., Calderwood, D. J., Waegell, W. O., Hirst, G. C. (2005). A-770041, a Novel and Selective Small-Molecule Inhibitor of Lck, Prevents Heart Allograft Rejection. J. Pharmacol. Exp. Ther. 315: 36-41 [Abstract] [Full Text]
Yang, Y., Villain, P., Mustelin, T., Couture, C. (2003). Critical Role of Ser-520 Phosphorylation for Membrane Recruitment and Activation of the ZAP-70 Tyrosine Kinase in T Cells. Mol. Cell. Biol. 23: 7667-7677 [Abstract] [Full Text]
Harding, S., Lipp, P., Alexander, D. R. (2002). A Therapeutic CD4 Monoclonal Antibody Inhibits TCR-{zeta} Chain Phosphorylation, {zeta}-Associated Protein of 70-kDa Tyr319 Phosphorylation, and TCR Internalization in Primary Human T Cells. J. Immunol. 169: 230-238 [Abstract] [Full Text]
Guntermann, C., Alexander, D. R. (2002). CTLA-4 Suppresses Proximal TCR Signaling in Resting Human CD4+ T Cells by Inhibiting ZAP-70 Tyr319 Phosphorylation: A Potential Role for Tyrosine Phosphatases. J. Immunol. 168: 4420-4429 [Abstract] [Full Text]
Ticchioni, M., Charvet, C., Noraz, N., Lamy, L., Steinberg, M., Bernard, A., Deckert, M. (2002). Signaling through ZAP-70 is required for CXCL12-mediated T-cell transendothelial migration. Blood 99: 3111-3118 [Abstract] [Full Text]
Magnan, A., Di Bartolo, V., Mura, A.-M., Boyer, C., Richelme, M., Lin, Y.-L., Roure, A., Gillet, A., Arrieumerlou, C., Acuto, O., Malissen, B., Malissen, M. (2001). T Cell Development and T Cell Responses in Mice with Mutations Affecting Tyrosines 292 or 315 of the Zap-70 Protein Tyrosine Kinase. JEM 194: 491-506 [Abstract] [Full Text]
Nervi, S., Atlan-Gepner, C., Kahn-Perles, B., Lecine, P., Vialettes, B., Imbert, J., Naquet, P. (2000). Specific Deficiency of p56lck Expression in T Lymphocytes from Type 1 Diabetic Patients. J. Immunol. 165: 5874-5883 [Abstract] [Full Text]
Michel, F., Mangino, G., Attal-Bonnefoy, G., Tuosto, L., Alcover, A., Roumier, A., Olive, D., Acuto, O. (2000). CD28 Utilizes Vav-1 to Enhance TCR-Proximal Signaling and NF-AT Activation. J. Immunol. 165: 3820-3829 [Abstract] [Full Text]
Nemorin, J.-G., Duplay, P. (2000). Evidence That Lck-mediated Phosphorylation of p56dok and p62dok May Play a Role in CD2 Signaling. J. Biol. Chem. 275: 14590-14597 [Abstract] [Full Text]
Ingley, E., Sarna, M. K., Beaumont, J. G., Tilbrook, P. A., Tsai, S., Takemoto, Y., Williams, J. H., Klinken, S. P. (2000). HS1 Interacts with Lyn and Is Critical for Erythropoietin-induced Differentiation of Erythroid Cells. J. Biol. Chem. 275: 7887-7893 [Abstract] [Full Text]
Denny, M. F., Patai, B., Straus, D. B. (2000). Differential T-Cell Antigen Receptor Signaling Mediated by the Src Family Kinases Lck and Fyn. Mol. Cell. Biol. 20: 1426-1435 [Abstract] [Full Text]
Singh, R. A. K., Zang, Y. C. Q., Shrivastava, A., Hong, J., Wang, G. T., Li, S., Tejada-Simon, M. V., Kozovska, M., Rivera, V. M., Zhang, J. Z. (1999). Th1 and Th2 Deviation of Myelin-Autoreactive T Cells by Altered Peptide Ligands Is Associated with Reciprocal Regulation of Lck, Fyn, and ZAP-70. J. Immunol. 163: 6393-6402 [Abstract] [Full Text]
Takemoto, Y., Furuta, M., Sato, M., Kubo, M., Hashimoto, Y. (1999). Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3. Int Immunol 11: 1957-1964 [Abstract] [Full Text]
Choi, Y. B., Kim, C. K., Yun, Y. (1999). Lad, an Adapter Protein Interacting with the SH2 Domain of p56lck, Is Required for T Cell Activation ,2. J. Immunol. 163: 5242-5249 [Abstract] [Full Text]
Veale, M., Raab, M., Li, Z., da Silva, A. J., Kraeft, S.-K., Weremowicz, S., Morton, C. C., Rudd, C. E. (1999). Novel Isoform of Lymphoid Adaptor FYN-T-binding Protein (FYB-130) Interacts with SLP-76 and Up-regulates Interleukin 2 Production. J. Biol. Chem. 274: 28427-28435 [Abstract] [Full Text]
Raab, M., Kang, H., da Silva, A., Zhu, X., Rudd, C. E. (1999). FYN-T-FYB-SLP-76 Interactions Define a T-cell Receptor zeta /CD3-mediated Tyrosine Phosphorylation Pathway That Up-regulates Interleukin 2 Transcription in T-cells. J. Biol. Chem. 274: 21170-21179 [Abstract] [Full Text]
Pelosi, M., Di Bartolo, V., Mounier, V., Mege, D., Pascussi, J.-M., Dufour, E., Blondel, A., Acuto, O. (1999). Tyrosine 319�in the Interdomain B of ZAP-70 Is a Binding Site for the Src Homology 2�Domain of Lck. J. Biol. Chem. 274: 14229-14237 [Abstract] [Full Text]
Ashe, J. M., Wiest, D. L., Abe, R., Singer, A. (1999). ZAP-70 Protein Promotes Tyrosine Phosphorylation of T Cell Receptor Signaling Motifs (ITAMs) in Immature CD4+8+ Thymocytes with Limiting p56lck. JEM 189: 1163-1168 [Abstract] [Full Text]
Di Bartolo, V., Mege, D., Germain, V., Pelosi, M., Dufour, E., Michel, F., Magistrelli, G., Isacchi, A., Acuto, O. (1999). Tyrosine 319,�a Newly Identified Phosphorylation Site of ZAP-70, Plays a Critical Role in T Cell Antigen Receptor Signaling. J. Biol. Chem. 274: 6285-6294 [Abstract] [Full Text]
Motoya, S., Kitamura, K., Matsuda, A., Maizel, A. L., Yamamoto, H., Takeda, A. (1999). Interaction between CD45-AP and Protein-tyrosine Kinases Involved in T Cell Receptor Signaling. J. Biol. Chem. 274: 1407-1414 [Abstract] [Full Text]
Michel, F., Grimaud, L., Tuosto, L., Acuto, O. (1998). Fyn and ZAP-70 Are Required for Vav Phosphorylation in T Cells Stimulated by Antigen-presenting Cells. J. Biol. Chem. 273: 31932-31938 [Abstract] [Full Text]
Sugawara, T., Di Bartolo, V., Miyazaki, T., Nakauchi, H., Acuto, O., Takahama, Y. (1998). An Improved Retroviral Gene Transfer Technique Demonstrates Inhibition of CD4-CD8- Thymocyte Development by Kinase-Inactive ZAP-70. J. Immunol. 161: 2888-2894 [Abstract] [Full Text]
Pacini, S., Ulivieri, C., Di Somma, M. M., Isacchi, A., Lanfrancone, L., Pelicci, P. G., Telford, J. L., Baldari, C. T. (1998). Tyrosine 474�of ZAP-70 Is Required for Association with the Shc Adaptor and for T-cell Antigen Receptor-dependent Gene Activation. J. Biol. Chem. 273: 20487-20493 [Abstract] [Full Text]
Liu, J., Kang, H., Raab, M., da Silva, A. J., Kraeft, S.-K., Rudd, C. E. (1998). FYB (FYN binding protein) serves as a binding partner for lymphoid protein and FYN kinase substrate SKAP55 and a SKAP55-related protein in T cells. Proc. Natl. Acad. Sci. USA 95: 8779-8784 [Abstract] [Full Text]
Zeitlmann, L., Knorr, T., Knoll, M., Romeo, C., Sirim, P., Kolanus, W. (1998). T Cell Activation Induced by Novel Gain-of-function Mutants of Syk and ZAP-70. J. Biol. Chem. 273: 15445-15452 [Abstract] [Full Text]
Huby, R. D.�J., Weiss, A., Ley, S. C. (1998). Nocodazole Inhibits Signal Transduction by the T Cell Antigen Receptor. J. Biol. Chem. 273: 12024-12031 [Abstract] [Full Text]
Wong, J., Straus, D., Chan, A. C. (1998). Genetic Evidence of a Role for Lck in T-Cell Receptor Function Independent or Downstream of ZAP-70/Syk Protein Tyrosine Kinases. Mol. Cell. Biol. 18: 2855-2866 [Abstract] [Full Text]
Sahuquillo, A. G., Roumier, A., Teixeiro, E., Bragado, R., Alarcon, B. (1998). T Cell Receptor (TCR) Engagement in Apoptosis-defective, but Interleukin 2 (IL-2)-producing, T Cells Results in Impaired ZAP70/CD3-{zeta} Association. JEM 187: 1179-1192 [Abstract] [Full Text]
Grazioli, L., Germain, V., Weiss, A., Acuto, O. (1998). Anti-peptide Antibodies Detect Conformational Changes of the Inter-SH2 Domain of ZAP-70 Due to Binding to the zeta �Chain and to Intramolecular Interactions. J. Biol. Chem. 273: 8916-8921 [Abstract] [Full Text]
Huby, R. D.J., Iwashima, M., Weiss, A., Ley, S. C. (1997). ZAP-70 Protein Tyrosine Kinase Is Constitutively Targeted to the T Cell Cortex Independently of its SH2 Domains. JCB 137: 1639-1649 [Abstract] [Full Text]
Wu, J., Zhao, Q., Kurosaki, T., Weiss, A. (1997). The Vav Binding Site (Y315) in ZAP-70 Is Critical for Antigen Receptor-mediated Signal Transduction. JEM 185: 1877-1882 [Abstract] [Full Text]
Mege, D., Di Bartolo, V., Germain, V., Tuosto, L., Michel, F., Acuto, O. (1996). Mutation of Tyrosines 492/493 in the Kinase Domain of ZAP-70 Affects Multiple T-cell Receptor Signaling Pathways. J. Biol. Chem. 271: 32644-32652 [Abstract] [Full Text]
Couture, C., Deckert, M., Williams, S., Russo, F. O., Altman, A., Mustelin, T. (1996). Identification of the Site in the Syk Protein Tyrosine Kinase That Binds the SH2 Domain of Lck. J. Biol. Chem. 271: 24294-24299 [Abstract] [Full Text]
Marie-Cardine, A., Fischer, S., Gorvel, J.-P., Maridonneau-Parini, I. (1996). Recruitment of Activated p56lck on Endosomes of CD2-triggered T Cells, Colocalization with ZAP-70. J. Biol. Chem. 271: 20734-20739 [Abstract] [Full Text]
Duplay, P., Alcover, A., Fargeas, C., Sekaly, R. P., Branton, P. E. (1996). An Activated Epidermal Growth Factor Receptor/Lck Chimera Restores Early T Cell Receptor-mediated Calcium Response in a CD45-deficient T Cell Line. J. Biol. Chem. 271: 17896-17902 [Abstract] [Full Text]
Isakov, N., Wange, R. L., Watts, J. D., Aebersold, R., Samelson, L. E. (1996). Purification and Characterization of Human ZAP-70 Protein-tyrosine Kinase from a Baculovirus Expression System. J. Biol. Chem. 271: 15753-15761 [Abstract] [Full Text]
Straus, D. B., Chan, A. C., Patai, B., Weiss, A. (1996). SH2 Domain Function Is Essential for the Role of the Lck Tyrosine Kinase in T Cell Receptor Signal Transduction. J. Biol. Chem. 271: 9976-9981 [Abstract] [Full Text]
August, A., Dupont, B. (1996). Association between Mitogen-activated Protein Kinase and the [IMAGE] Chain of the T Cell Receptor (TcR) with the SH2,3 Domain of p56[IMAGE]. J. Biol. Chem. 271: 10054-10059 [Abstract] [Full Text]
Collette, Y., Dutartre, H., Benziane, A., Ramos-Morales, F., Benarous, R., Harris, M., Olive, D. (1996). Physical and Functional Interaction of Nef with Lck. J. Biol. Chem. 271: 6333-6341 [Abstract] [Full Text]
Jung, J. U., Lang, S. M., Friedrich, U., Jun, T., Roberts, T. M., Desrosiers, R. C., Biesinger, B. (1995). Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri. J. Biol. Chem. 270: 20660-20667 [Abstract] [Full Text]
Aoki, Y., Kim, Y.-T., Stillwell, R., Kim, T. J., Pillai, S. (1995). The SH2 Domains of Src Family Kinases Associate with Syk. J. Biol. Chem. 270: 15658-15663 [Abstract] [Full Text]
Wange, R. L., Isakov, N., Burke, T. R. Jr., Otaka, A., Roller, P. P., Watts, J. D., Aebersold, R., Samelson, L. E. (1995). F(2)(Pmp)(2)-TAM[IMAGE](3), a Novel Competitive Inhibitor of the Binding of ZAP-70 to the T Cell Antigen Receptor, Blocks Early T Cell Signaling. J. Biol. Chem. 270: 944-948 [Abstract] [Full Text]