The combination of anti-B7 monoclonal antibody and cyclosporin A induces alloantigen-specific anergy during a primary mixed lymphocyte reaction

doi:10.1084/jem.179.2.715

This Article

	Full Text (PDF, 622K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Van Gool, S. W.
	Articles by Ceuppens, J. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Van Gool, S. W.
	Articles by Ceuppens, J. L.


Social Bookmarking

	What's this?

ARTICLES

The combination of anti-B7 monoclonal antibody and cyclosporin A induces alloantigen-specific anergy during a primary mixed lymphocyte reaction

SW Van Gool, M de Boer and JL Ceuppens
Department of Internal Medicine, Catholic University of Leuven, Belgium.

Interaction of CD28/CTLA-4 on T cells with B7 on antigen-presenting cells constitutes an important costimulatory signal for T cells and is responsible for cyclosporin A-resistant interleukin 2 (IL-2) gene expression and potentially also for prevention of anergy induction after T cell receptor triggering. In this paper, we demonstrate that addition of a monoclonal antibody to B7, which blocks B7-CD28/CTLA-4 interaction, and of cyclosporin A together, but not separately, to a primary mixed lymphocyte reaction of freshly isolated human T cells towards a human B cell line, induces nonresponsiveness of alloantigen- specific cytotoxic T lymphocyte precursors, whereas reactivity to a third party stimulator is intact. Nonresponsiveness could be reversed by culture in IL-2, indicating that anergy, and not clonal deletion, is responsible for this phenomenon. Our finding opens important perspectives for the development of new therapeutic strategies in transplantation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Vermeiren, J., Ceuppens, J. L., Van Ghelue, M., Witters, P., Bullens, D., Mages, H. W., Kroczek, R. A., Van Gool, S. W. (2004). Human T Cell Activation by Costimulatory Signal-Deficient Allogeneic Cells Induces Inducible Costimulator-Expressing Anergic T Cells with Regulatory Cell Activity. J. Immunol. 172: 5371-5378 [Abstract] [Full Text]
Peng, J., Liu, C., Liu, D., Ren, C., Li, W., Wang, Z., Xing, N., Xu, C., Chen, X., Ji, C., Zhang, M., Hou, M. (2003). Effects of B7-blocking agent and/or CsA on induction of platelet-specific T-cell anergy in chronic autoimmune thrombocytopenic purpura. Blood 101: 2721-2726 [Abstract] [Full Text]
Ng, W. F., Hernandez-Fuentes, M., Baker, R., Chaudhry, A., Lechler, R. I. (2002). Reversibility with Interleukin-2 Suggests that T Cell Anergy Contributes to Donor-Specific Hyporesponsiveness in Renal Transplant Patients. J. Am. Soc. Nephrol. 13: 2983-2989 [Abstract] [Full Text]
Wu, Z.-Q., Khan, A. Q., Shen, Y., Schartman, J., Peach, R., Lees, A., Mond, J. J., Gause, W. C., Snapper, C. M. (2000). B7 Requirements for Primary and Secondary Protein- and Polysaccharide-Specific Ig Isotype Responses to Streptococcus pneumoniae. J. Immunol. 165: 6840-6848 [Abstract] [Full Text]
Frauwirth, K. A., Alegre, M.-L., Thompson, C. B. (2000). Induction of T Cell Anergy in the Absence of CTLA-4/B7 Interaction. J. Immunol. 164: 2987-2993 [Abstract] [Full Text]
Taylor, P. A., Panoskaltsis-Mortari, A., Noelle, R. J., Blazar, B. R. (2000). Analysis of the Requirements for the Induction of CD4+ T Cell Alloantigen Hyporesponsiveness by Ex Vivo Anti-CD40 Ligand Antibody. J. Immunol. 164: 612-622 [Abstract] [Full Text]
Van Hoffen, E., Van Wichen, D. F., Leemans, J. C., Broekhuizen, R. A.J.F., Bruggink, A. H., De Boer, M., De Jonge, N., Kirkels, H., Slootweg, P. J., Gmelig-Meyling, F. H.J., De Weger, R. A. (1998). T Cell Apoptosis in Human Heart Allografts : Association with Lack of Co-Stimulation?. Am. J. Pathol. 153: 1813-1824 [Abstract] [Full Text]
Chai, J.-G., Bartok, I., Scott, D., Dyson, J., Lechler, R. (1998). T:T Antigen Presentation by Activated Murine CD8+ T Cells Induces Anergy and Apoptosis. J. Immunol. 160: 3655-3665 [Abstract] [Full Text]