Enhanced antigen presentation in the absence of the invariant chain endosomal localization signal

doi:10.1084/jem.178.6.1959

This Article

	Full Text (PDF, 1464K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Anderson, M. S.
	Articles by Miller, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Anderson, M. S.
	Articles by Miller, J.


Social Bookmarking

	What's this?

ARTICLES

Enhanced antigen presentation in the absence of the invariant chain endosomal localization signal

MS Anderson, K Swier, L Arneson and J Miller
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

The cytosolic tail of the major histocompatibility complex class II- associated invariant chain (Ii) molecule is thought to contain the endosomal localization signal that directs and/or retains newly synthesized class II within the endosomal antigen processing compartment. To determine the role of this signal in class II transport and antigen presentation we have generated class II-positive L cell transfectants that coexpress wild type or truncated forms of Ii. Deletion of the endosomal localization signal from Ii results in rapid transport of class II-Ii complexes to the cell surface. Once at the cell surface, the complex is efficiently internalized, Ii is degraded, and class II free of Ii is recycled back to the plasma membrane. Interestingly, the truncated form of Ii is still able to increase the efficiency of antigen presentation to T cells. These data suggest that the ability of Ii to enhance antigen presentation is not limited to Golgi apparatus-endosomal sorting and raise the possibility that endocytosed class II can form immunogenic complexes with newly processed antigen.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Walseng, E., Bakke, O., Roche, P. A. (2008). Major Histocompatibility Complex Class II-Peptide Complexes Internalize Using a Clathrin- and Dynamin-independent Endocytosis Pathway. J. Biol. Chem. 283: 14717-14727 [Abstract] [Full Text]
Graf, B., Bushnell, T., Miller, J. (2007). LFA-1-Mediated T Cell Costimulation through Increased Localization of TCR/Class II Complexes to the Central Supramolecular Activation Cluster and Exclusion of CD45 from the Immunological Synapse. J. Immunol. 179: 1616-1624 [Abstract] [Full Text]
Khalil, H., Brunet, A., Thibodeau, J. (2005). A three-amino-acid-long HLA-DR{beta} cytoplasmic tail is sufficient to overcome ER retention of invariant-chain p35. J. Cell Sci. 118: 4679-4687 [Abstract] [Full Text]
Dissanayake, S. K., Tuera, N., Ostrand-Rosenberg, S. (2005). Presentation of Endogenously Synthesized MHC Class II-Restricted Epitopes by MHC Class II Cancer Vaccines Is Independent of Transporter Associated with Ag Processing and the Proteasome. J. Immunol. 174: 1811-1819 [Abstract] [Full Text]
Khalil, H., Brunet, A., Saba, I., Terra, R., Sekaly, R. P., Thibodeau, J. (2003). The MHC class II {beta} chain cytoplasmic tail overcomes the invariant chain p35-encoded endoplasmic reticulum retention signal. Int Immunol 15: 1249-1263 [Abstract] [Full Text]
Ashman, J. B., Miller, J. (1999). A Role for the Transmembrane Domain in the Trimerization of the MHC Class II-Associated Invariant Chain. J. Immunol. 163: 2704-2712 [Abstract] [Full Text]
Arunachalam, B., Pan, M., Cresswell, P. (1998). Intracellular Formation and Cell Surface Expression of a Complex of an Intact Lysosomal Protein and MHC Class II Molecules. J. Immunol. 160: 5797-5806 [Abstract] [Full Text]
Aichinger, G., Karlsson, L., Jackson, M. R., Vestberg, M., Vaughan, J. H., Teyton, L., Lechler, R. I., Peterson, P. A. (1997). Major Histocompatibility Complex Class II-dependent Unfolding, Transport, and Degradation of Endogenous Proteins. J. Biol. Chem. 272: 29127-29136 [Abstract] [Full Text]