The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 176, 1025-1031, Copyright © 1992 by Rockefeller University Press

ARTICLES

Differential structure-function requirements of the transmembranal domain of the B cell antigen receptor

VS Parikh, GA Bishop, KJ Liu, BT Do, MR Ghosh, BS Kim and PW Tucker
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

By generating phosphorylcholine (PC)-specific, wild-type (mu), and chimeric (mu-I-A alpha) antigen receptor transfectants of mature B cells, we have shown that the COOH terminus of the mu heavy chain is essential for three major functions: immediate signal transduction (measured as changes in intracellular Ca2+), antigen presentation, and induction of immunoglobulin M secretion. A more detailed analysis of structural requirements of the COOH-terminal domains contributing to these functions was achieved by systematically replacing the spacer, cytoplasmic, and transmembranal domains of the mu-I-A alpha chimeric chain with those of mu. Using this rescue approach, we show that the carboxyl two-thirds of the transmembranal domain (proximal to the cytoplasmic domain) is required for induction of intracellular Ca2+, whereas the complete transmembranal domain is required for the function of antigen presentation but is dispensable for induction of antibody secretion.
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