Identification of the low affinity receptor for immunoglobulin E on mouse mast cells and macrophages as Fc gamma RII and Fc gamma RIII

doi:10.1084/jem.176.2.469

This Article

	Full Text (PDF, 721K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Takizawa, F.
	Articles by Kinet, J. P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Takizawa, F.
	Articles by Kinet, J. P.


Social Bookmarking

	What's this?

ARTICLES

Identification of the low affinity receptor for immunoglobulin E on mouse mast cells and macrophages as Fc gamma RII and Fc gamma RIII

F Takizawa, M Adamczewski and JP Kinet
Molecular Allergy and Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852.

In addition to their well characterized high affinity immunoglobulin E (IgE) receptors (Fc epsilon RI) mast cells have long been suspected to express undefined Fc receptors capable of binding IgE with low affinity. In this paper, we show that Fc gamma RII and Fc gamma RIII, but not Mac-2, on mouse mast cells and macrophages bind IgE-immune complexes. This binding is efficiently competed by 2.4G2, a monoclonal antibody against the extracellular homologous region of both Fc gamma RII and Fc gamma RIII. Furthermore, IgE-immune complexes bind specifically to Fc gamma RII or Fc gamma RIII transfected into COS-7 cells. The association constants of IgE binding estimated from competition experiments are about 3.1 x 10(5) M-1 for Fc gamma RII, and 4.8 x 10(5) M-1 for Fc gamma RIII. Engagement of Fc gamma RII and Fc gamma RIII with IgE-immune complexes (after blocking access to Fc epsilon RI) or with IgG-immune complexes triggers C57.1 mouse mast cells to release serotonin. This release is inhibited by 2.4G2, and at maximum, reaches 30-40% of the intracellular content, about half of the maximal release (60-80%) obtained after Fc epsilon RI engagement. These data demonstrate that mouse Fc gamma RII and Fc gamma RIII are not isotype specific, and that the binding of IgE-immune complexes to these receptors induces cell activation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Bruhns, P., Iannascoli, B., England, P., Mancardi, D. A., Fernandez, N., Jorieux, S., Daeron, M. (2009). Specificity and affinity of human Fc{gamma} receptors and their polymorphic variants for human IgG subclasses. Blood 113: 3716-3725 [Abstract] [Full Text]
Sun, J., Arias, K., Alvarez, D., Fattouh, R., Walker, T., Goncharova, S., Kim, B., Waserman, S., Reed, J., Coyle, A. J., Jordana, M. (2007). Impact of CD40 Ligand, B Cells, and Mast Cells in Peanut-Induced Anaphylactic Responses. J. Immunol. 179: 6696-6703 [Abstract] [Full Text]
Cao, L., Yu, K., Banh, C., Nguyen, V., Ritz, A., Raphael, B. J., Kawakami, Y., Kawakami, T., Salomon, A. R. (2007). Quantitative Time-Resolved Phosphoproteomic Analysis of Mast Cell Signaling. J. Immunol. 179: 5864-5876 [Abstract] [Full Text]
Gurish, M. F., Bryce, P. J., Tao, H., Kisselgof, A. B., Thornton, E. M., Miller, H. R., Friend, D. S., Oettgen, H. C. (2004). IgE Enhances Parasite Clearance and Regulates Mast Cell Responses in Mice Infected with Trichinella spiralis. J. Immunol. 172: 1139-1145 [Abstract] [Full Text]
Taube, C., Dakhama, A., Rha, Y.-H., Takeda, K., Joetham, A., Park, J.-W., Balhorn, A., Takai, T., Poch, K. R., Nick, J. A., Gelfand, E. W. (2003). Transient Neutrophil Infiltration After Allergen Challenge Is Dependent on Specific Antibodies and Fc{gamma}III Receptors. J. Immunol. 170: 4301-4309 [Abstract] [Full Text]
Arase, N., Arase, H., Hirano, S., Yokosuka, T., Sakurai, D., Saito, T. (2003). IgE-Mediated Activation of NK Cells Through Fc{gamma}RIII. J. Immunol. 170: 3054-3058 [Abstract] [Full Text]
Tanaka, S., Takasu, Y., Mikura, S., Satoh, N., Ichikawa, A. (2002). Antigen-independent Induction of Histamine Synthesis by Immunoglobulin E in Mouse Bone Marrow-derived Mast Cells. JEM 196: 229-235 [Abstract] [Full Text]
Karlsson, M. C. I., Getahun, A., Heyman, B. (2001). Fc{gamma}RIIB in IgG-Mediated Suppression of Antibody Responses: Different Impact In Vivo and In Vitro. J. Immunol. 167: 5558-5564 [Abstract] [Full Text]
Daheshia, M., Friend, D. S., Grusby, M. J., Austen, K. F., Katz, H. R. (2001). Increased Severity of Local and Systemic Anaphylactic Reactions in Gp49b1-Deficient Mice. JEM 194: 227-234 [Abstract] [Full Text]
Chen, M., Aosai, F., Mun, H.-S., Norose, K., Hata, H., Yano, A. (2000). Anti-HSP70 Autoantibody Formation by B-1 Cells in Toxoplasma gondii-Infected Mice. Infect. Immun. 68: 4893-4899 [Abstract] [Full Text]
Wernersson, S., Karlsson, M. C. I., Dahlstrom, J., Mattsson, R., Verbeek, J. S., Heyman, B. (1999). IgG-Mediated Enhancement of Antibody Responses Is Low in Fc Receptor {gamma} Chain-Deficient Mice and Increased in Fc{gamma}RII-Deficient Mice. J. Immunol. 163: 618-622 [Abstract] [Full Text]
Ujike, A., Ishikawa, Y., Ono, M., Yuasa, T., Yoshino, T., Fukumoto, M., Ravetch, J. V., Takai, T. (1999). Modulation of Immunoglobulin (Ig)E-mediated Systemic Anaphylaxis by Low-Affinity Fc Receptors for IgG. JEM 189: 1573-1579 [Abstract] [Full Text]
Karlsson, M. C.�I., Wernersson, S., Diaz de Stahl, T., Gustavsson, S., Heyman, B. (1999). Efficient IgG-mediated suppression of primary antibody responses in Fcgamma receptor-deficient mice. Proc. Natl. Acad. Sci. USA 96: 2244-2249 [Abstract] [Full Text]
MacGlashan, D. Jr, McKenzie-White, J., Chichester, K., Bochner, B. S., Davis, F. M., Schroeder, J. T., Lichtenstein, L. M. (1998). In Vitro Regulation of Fc&b.epsi;RIalpha Expression on Human Basophils by IgE Antibody. Blood 91: 1633-1643 [Abstract] [Full Text]
Yamaguchi, M., Lantz, C. S., Oettgen, H. C., Katona, I. M., Fleming, T., Miyajima, I., Kinet, J.-P., Galli, S. J. (1997). IgE Enhances Mouse Mast Cell Fc{varepsilon}RI Expression In Vitro and In Vivo: Evidence for a Novel Amplification Mechanism in IgE-dependent Reactions. JEM 185: 663-672 [Abstract] [Full Text]
Radaev, S., Sun, P. D. (2001). Recognition of IgG by Fcgamma Receptor. THE ROLE OF Fc GLYCOSYLATION AND THE BINDING OF PEPTIDE INHIBITORS. J. Biol. Chem. 276: 16478-16483 [Abstract] [Full Text]