Localization of Epstein-Barr virus cytotoxic T cell epitopes using recombinant vaccinia: implications for vaccine development

doi:10.1084/jem.176.1.169

This Article

	Full Text (PDF, 766K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Khanna, R.
	Articles by Moss, D. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Khanna, R.
	Articles by Moss, D. J.


Social Bookmarking

	What's this?

ARTICLES

Localization of Epstein-Barr virus cytotoxic T cell epitopes using recombinant vaccinia: implications for vaccine development

R Khanna, SR Burrows, MG Kurilla, CA Jacob, IS Misko, TB Sculley, E Kieff and DJ Moss
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.

There is considerable interest in designing an effective vaccine to the ubiquitous Epstein-Barr virus (EBV). An important role for EBV-specific cytotoxic T lymphocytes (CTLs) in eliminating virus-infected cells is well established. Limited studies using a small number of immune donors have defined target epitopes within the latent antigens of EBV. The present study provides an extensive analysis of the distribution of class I-restricted CTL epitopes within EBV-encoded proteins. Using recombinant vaccinia encoding individual EBV latent antigens (Epstein- Barr nuclear antigen [EBNA] 1, 2, 3A, 3B, 3C, LP, and LMP 1), we have successfully localized target epitopes recognized by CTL clones from a panel of 14 EBV-immune donors. Of the 20 CTL epitopes localized, five were defined at the peptide level. Although CTL clones specific for nine epitopes recognized both type 1 and type 2 transformants, a significant number of epitopes (7/16 epitopes for which EBV type specificity was determined) were detected only on type 1 EBV transformants. Vaccinia recombinants encoding EBNA 3A and EBNA 3C were recognized more frequently than any other vaccinia recombinants used in this study, while no CTL epitopes were localized in EBNA 1. Surprisingly, epitope specificity for a large number of EBV-specific CTL clones could not be localized, although vaccinia recombinants used in this study encoded most of the latent antigens of EBV. These results suggest that any EBV vaccine based on CTL epitopes designed to provide widespread protection will need to include not only latent antigen sequences but also other regions of the genome. The apparent inability of human CTLs to recognize EBNA 1 as a target antigen, often the only latent antigen expressed in Burkitt's lymphoma and nasopharyngeal carcinoma, suggests that EBV-specific CTL control of these tumors will not be feasible unless the down-regulation of latent antigens can be reversed.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Yuling, H., Ruijing, X., Li, L., Xiang, J., Rui, Z., Yujuan, W., Lijun, Z., Chunxian, D., Xinti, T., Wei, X., Lang, C., Yanping, J., Tao, X., Mengjun, W., Jie, X., Youxin, J., Jinquan, T. (2009). EBV-Induced Human CD8+ NKT Cells Suppress Tumorigenesis by EBV-Associated Malignancies. Cancer Res. 69: 7935-7944 [Abstract] [Full Text]
Rist, M., Smith, C., Bell, M. J., Burrows, S. R., Khanna, R. (2009). Cross-recognition of HLA DR4 alloantigen by virus-specific CD8+ T cells: a new paradigm for self-/nonself-recognition. Blood 114: 2244-2253 [Abstract] [Full Text]
Smith, C., Wakisaka, N., Crough, T., Peet, J., Yoshizaki, T., Beagley, L., Khanna, R. (2009). Discerning regulation of cis- and trans-presentation of CD8+ T-cell epitopes by EBV-encoded oncogene LMP-1 through self-aggregation. Blood 113: 6148-6152 [Abstract] [Full Text]
Brooks, J. M., Lee, S. P., Leese, A. M., Thomas, W. A., Rowe, M., Rickinson, A. B. (2009). Cyclical Expression of EBV Latent Membrane Protein 1 in EBV-Transformed B Cells Underpins Heterogeneity of Epitope Presentation and CD8+ T Cell Recognition. J. Immunol. 182: 1919-1928 [Abstract] [Full Text]
Wynn, K. K., Fulton, Z., Cooper, L., Silins, S. L., Gras, S., Archbold, J. K., Tynan, F. E., Miles, J. J., McCluskey, J., Burrows, S. R., Rossjohn, J., Khanna, R. (2008). Impact of clonal competition for peptide-MHC complexes on the CD8+ T-cell repertoire selection in a persistent viral infection. Blood 111: 4283-4292 [Abstract] [Full Text]
Liu, A., Claesson, H.-E., Mahshid, Y., Klein, G., Klein, E. (2008). Leukotriene B4 activates T cells that inhibit B-cell proliferation in EBV-infected cord blood-derived mononuclear cell cultures. Blood 111: 2693-2703 [Abstract] [Full Text]
Haigh, T. A., Lin, X., Jia, H., Hui, E. P., Chan, A. T. C., Rickinson, A. B., Taylor, G. S. (2008). EBV Latent Membrane Proteins (LMPs) 1 and 2 as Immunotherapeutic Targets: LMP-Specific CD4+ Cytotoxic T Cell Recognition of EBV-Transformed B Cell Lines. J. Immunol. 180: 1643-1654 [Abstract] [Full Text]
Bhaduri-McIntosh, S., Rotenberg, M. J., Gardner, B., Robert, M., Miller, G. (2008). Repertoire and frequency of immune cells reactive to Epstein-Barr virus-derived autologous lymphoblastoid cell lines. Blood 111: 1334-1343 [Abstract] [Full Text]
Niens, M., Jarrett, R. F., Hepkema, B., Nolte, I. M., Diepstra, A., Platteel, M., Kouprie, N., Delury, C. P., Gallagher, A., Visser, L., Poppema, S., te Meerman, G. J., van den Berg, A. (2007). HLA-A*02 is associated with a reduced risk and HLA-A*01 with an increased risk of developing EBV+ Hodgkin lymphoma. Blood 110: 3310-3315 [Abstract] [Full Text]
Tellam, J., Fogg, M. H., Rist, M., Connolly, G., Tscharke, D., Webb, N., Heslop, L., Wang, F., Khanna, R. (2007). Influence of translation efficiency of homologous viral proteins on the endogenous presentation of CD8+ T cell epitopes. JEM 204: 525-532 [Abstract] [Full Text]
Ito, Y., Demachi-Okamura, A., Ohta, R., Akatsuka, Y., Nishida, K., Tsujimura, K., Morishima, Y., Takahashi, T., Kuzushima, K. (2007). Full-length EBNA1 mRNA-transduced dendritic cells stimulate cytotoxic T lymphocytes recognizing a novel HLA-Cw*0303- and -Cw*0304-restricted epitope on EBNA1-expressing cells. J. Gen. Virol. 88: 770-780 [Abstract] [Full Text]
Chen, A., Zhao, B., Kieff, E., Aster, J. C., Wang, F. (2006). EBNA-3B- and EBNA-3C-Regulated Cellular Genes in Epstein-Barr Virus-Immortalized Lymphoblastoid Cell Lines.. J. Virol. 80: 10139-10150 [Abstract] [Full Text]
Smith, C., Cooper, L., Burgess, M., Rist, M., Webb, N., Lambley, E., Tellam, J., Marlton, P., Seymour, J. F., Gandhi, M., Khanna, R. (2006). Functional Reversion of Antigen-Specific CD8+ T Cells from Patients with Hodgkin Lymphoma following In Vitro Stimulation with Recombinant Polyepitope. J. Immunol. 177: 4897-4906 [Abstract] [Full Text]
Taylor, G. S., Long, H. M., Haigh, T. A., Larsen, M., Brooks, J., Rickinson, A. B. (2006). A Role for Intercellular Antigen Transfer in the Recognition of EBV-Transformed B Cell Lines by EBV Nuclear Antigen-Specific CD4+ T Cells. J. Immunol. 177: 3746-3756 [Abstract] [Full Text]
Fogg, M. H., Garry, D., Awad, A., Wang, F., Kaur, A. (2006). The BZLF1 Homolog of an Epstein-Barr-Related {gamma}-Herpesvirus Is a Frequent Target of the CTL Response in Persistently Infected Rhesus Macaques. J. Immunol. 176: 3391-3401 [Abstract] [Full Text]
Fogg, M. H., Kaur, A., Cho, Y.-G., Wang, F. (2005). The CD8+ T-Cell Response to an Epstein-Barr Virus-Related Gammaherpesvirus Infecting Rhesus Macaques Provides Evidence for Immune Evasion by the EBNA-1 Homologue. J. Virol. 79: 12681-12691 [Abstract] [Full Text]
Ressing, M. E., Keating, S. E., van Leeuwen, D., Koppers-Lalic, D., Pappworth, I. Y., Wiertz, E. J. H. J., Rowe, M. (2005). Impaired Transporter Associated with Antigen Processing-Dependent Peptide Transport during Productive EBV Infection. J. Immunol. 174: 6829-6838 [Abstract] [Full Text]
Gudgeon, N. H., Taylor, G. S., Long, H. M., Haigh, T. A., Rickinson, A. B. (2005). Regression of Epstein-Barr Virus-Induced B-Cell Transformation In Vitro Involves Virus-Specific CD8+ T Cells as the Principal Effectors and a Novel CD4+ T-Cell Reactivity. J. Virol. 79: 5477-5488 [Abstract] [Full Text]
Touitou, R., O'Nions, J., Heaney, J., Allday, M. J. (2005). Epstein-Barr virus EBNA3 proteins bind to the C8/{alpha}7 subunit of the 20S proteasome and are degraded by 20S proteasomes in vitro, but are very stable in latently infected B cells. J. Gen. Virol. 86: 1269-1277 [Abstract] [Full Text]
Long, H. M., Haigh, T. A., Gudgeon, N. H., Leen, A. M., Tsang, C.-W., Brooks, J., Landais, E., Houssaint, E., Lee, S. P., Rickinson, A. B., Taylor, G. S. (2005). CD4+ T-Cell Responses to Epstein-Barr Virus (EBV) Latent-Cycle Antigens and the Recognition of EBV-Transformed Lymphoblastoid Cell Lines. J. Virol. 79: 4896-4907 [Abstract] [Full Text]
Sutkowski, N., Chen, G., Calderon, G., Huber, B. T. (2004). Epstein-Barr Virus Latent Membrane Protein LMP-2A Is Sufficient for Transactivation of the Human Endogenous Retrovirus HERV-K18 Superantigen. J. Virol. 78: 7852-7860 [Abstract] [Full Text]
Lee, S. P., Brooks, J. M., Al-Jarrah, H., Thomas, W. A., Haigh, T. A., Taylor, G. S., Humme, S., Schepers, A., Hammerschmidt, W., Yates, J. L., Rickinson, A. B., Blake, N. W. (2004). CD8 T Cell Recognition of Endogenously Expressed Epstein-Barr Virus Nuclear Antigen 1. JEM 199: 1409-1420 [Abstract] [Full Text]
Duraiswamy, J., Bharadwaj, M., Tellam, J., Connolly, G., Cooper, L., Moss, D., Thomson, S., Yotnda, P., Khanna, R. (2004). Induction of Therapeutic T-Cell Responses to Subdominant Tumor-associated Viral Oncogene after Immunization with Replication-incompetent Polyepitope Adenovirus Vaccine. Cancer Res. 64: 1483-1489 [Abstract] [Full Text]
Landais, E., Saulquin, X., Scotet, E., Trautmann, L., Peyrat, M.-A., Yates, J. L., Kwok, W. W., Bonneville, M., Houssaint, E. (2004). Direct killing of Epstein-Barr virus (EBV)-infected B cells by CD4 T cells directed against the EBV lytic protein BHRF1. Blood 103: 1408-1416 [Abstract] [Full Text]
Thompson, M. P., Kurzrock, R. (2004). Epstein-Barr Virus and Cancer. Clin. Cancer Res. 10: 803-821 [Abstract] [Full Text]
Bickham, K., Goodman, K., Paludan, C., Nikiforow, S., Tsang, M. L., Steinman, R. M., Munz, C. (2003). Dendritic Cells Initiate Immune Control of Epstein-Barr Virus Transformation of B Lymphocytes In Vitro. JEM 198: 1653-1663 [Abstract] [Full Text]
Nikiforow, S., Bottomly, K., Miller, G., Munz, C. (2003). Cytolytic CD4+-T-Cell Clones Reactive to EBNA1 Inhibit Epstein-Barr Virus-Induced B-Cell Proliferation. J. Virol. 77: 12088-12104 [Abstract] [Full Text]
Burrows, S. R., Elkington, R. A., Miles, J. J., Green, K. J., Walker, S., Haryana, S. M., Moss, D. J., Dunckley, H., Burrows, J. M., Khanna, R. (2003). Promiscuous CTL Recognition of Viral Epitopes on Multiple Human Leukocyte Antigens: Biological Validation of the Proposed HLA A24 Supertype. J. Immunol. 171: 1407-1412 [Abstract] [Full Text]
Chakrabarti, S., Milligan, D. W., Pillay, D., Mackinnon, S., Holder, K., Kaur, N., McDonald, D., Fegan, C. D., Waldmann, H., Hale, G., Rickinson, A., Steven, N. (2003). Reconstitution of the Epstein-Barr virus-specific cytotoxic T-lymphocyte response following T-cell-depleted myeloablative and nonmyeloablative allogeneic stem cell transplantation. Blood 102: 839-842 [Abstract] [Full Text]
Duraiswamy, J., Burrows, J. M., Bharadwaj, M., Burrows, S. R., Cooper, L., Pimtanothai, N., Khanna, R. (2003). Ex Vivo Analysis of T-Cell Responses to Epstein-Barr Virus-Encoded Oncogene Latent Membrane Protein 1 Reveals Highly Conserved Epitope Sequences in Virus Isolates from Diverse Geographic Regions. J. Virol. 77: 7401-7410 [Abstract] [Full Text]
Elkington, R., Walker, S., Crough, T., Menzies, M., Tellam, J., Bharadwaj, M., Khanna, R. (2003). Ex Vivo Profiling of CD8+-T-Cell Responses to Human Cytomegalovirus Reveals Broad and Multispecific Reactivities in Healthy Virus Carriers. J. Virol. 77: 5226-5240 [Abstract] [Full Text]
Duraiswamy, J., Sherritt, M., Thomson, S., Tellam, J., Cooper, L., Connolly, G., Bharadwaj, M., Khanna, R. (2003). Therapeutic LMP1 polyepitope vaccine for EBV-associated Hodgkin disease and nasopharyngeal carcinoma. Blood 101: 3150-3156 [Abstract] [Full Text]
Lautscham, G., Haigh, T., Mayrhofer, S., Taylor, G., Croom-Carter, D., Leese, A., Gadola, S., Cerundolo, V., Rickinson, A., Blake, N. (2003). Identification of a TAP-Independent, Immunoproteasome-Dependent CD8+ T-Cell Epitope in Epstein-Barr Virus Latent Membrane Protein 2. J. Virol. 77: 2757-2761 [Abstract] [Full Text]
Sitki-Green, D., Covington, M., Raab-Traub, N. (2003). Compartmentalization and Transmission of Multiple Epstein-Barr Virus Strains in Asymptomatic Carriers. J. Virol. 77: 1840-1847 [Abstract] [Full Text]
Lin, C.-L., Lo, W.-F., Lee, T.-H., Ren, Y., Hwang, S.-L., Cheng, Y.-F., Chen, C.-L., Chang, Y.-S., Lee, S. P., Rickinson, A. B., Tam, P. K. H. (2002). Immunization with Epstein-Barr Virus (EBV) Peptide-pulsed Dendritic Cells Induces Functional CD8+ T-Cell Immunity and May Lead to Tumor Regression in Patients with EBV-positive Nasopharyngeal Carcinoma. Cancer Res. 62: 6952-6958 [Abstract] [Full Text]
Tao, Q., Yang, J., Huang, H., Swinnen, L. J., Ambinder, R. F. (2002). Conservation of Epstein-Barr Virus Cytotoxic T-Cell Epitopes in Posttransplant Lymphomas : Implications for Immune Therapy. Am. J. Pathol. 160: 1839-1845 [Abstract] [Full Text]
Pai, S., O'Sullivan, B. J., Cooper, L., Thomas, R., Khanna, R. (2002). RelB Nuclear Translocation Mediated by C-Terminal Activator Regions of Epstein-Barr Virus-Encoded Latent Membrane Protein 1 and Its Effect on Antigen-Presenting Function in B Cells. J. Virol. 76: 1914-1921 [Abstract] [Full Text]
Groves, A. K., Cotter, M. A., Subramanian, C., Robertson, E. S. (2001). The Latency-Associated Nuclear Antigen Encoded by Kaposi's Sarcoma-Associated Herpesvirus Activates Two Major Essential Epstein-Barr Virus Latent Promoters. J. Virol. 75: 9446-9457 [Abstract] [Full Text]
Leen, A., Meij, P., Redchenko, I., Middeldorp, J., Bloemena, E., Rickinson, A., Blake, N. (2001). Differential Immunogenicity of Epstein-Barr Virus Latent-Cycle Proteins for Human CD4+ T-Helper 1 Responses. J. Virol. 75: 8649-8659 [Abstract] [Full Text]
Chapman, A. L. N., Rickinson, A. B., Thomas, W. A., Jarrett, R. F., Crocker, J., Lee, S. P. (2001). Epstein-Barr Virus-specific Cytotoxic T Lymphocyte Responses in the Blood and Tumor Site of Hodgkin's Disease Patients: Implications for a T-cell-based Therapy. Cancer Res. 61: 6219-6226 [Abstract] [Full Text]
Trivedi, P., Spinsanti, P., Cuomo, L., Volpe, M., Takada, K., Frati, L., Faggioni, A. (2001). Differential Regulation of Epstein-Barr Virus (EBV) Latent Gene Expression in Burkitt Lymphoma Cells Infected with a Recombinant EBV Strain. J. Virol. 75: 4929-4935 [Abstract] [Full Text]
Gottschalk, S., Ng, C. Y. C., Perez, M., Smith, C. A., Sample, C., Brenner, M. K., Heslop, H. E., Rooney, C. M. (2001). An Epstein-Barr virus deletion mutant associated with fatal lymphoproliferative disease unresponsive to therapy with virus-specific CTLs. Blood 97: 835-843 [Abstract] [Full Text]
Blake, N., Haigh, T., Shaka'a, G., Croom-Carter, D., Rickinson, A. (2000). The Importance of Exogenous Antigen in Priming the Human CD8+ T Cell Response: Lessons from the EBV Nuclear Antigen EBNA1. J. Immunol. 165: 7078-7087 [Abstract] [Full Text]
Annels, N. E., Callan, M. F. C., Tan, L., Rickinson, A. B. (2000). Changing Patterns of Dominant TCR Usage with Maturation of an EBV-Specific Cytotoxic T Cell Response. J. Immunol. 165: 4831-4841 [Abstract] [Full Text]
Steigerwald-Mullen, P., Kurilla, M. G., Braciale, T. J. (2000). Type 2 Cytokines Predominate in the Human CD4+ T-Lymphocyte Response to Epstein-Barr Virus Nuclear Antigen 1. J. Virol. 74: 6748-6759 [Abstract] [Full Text]
Jiang, H., Cho, Y.-g., Wang, F. (2000). Structural, Functional, and Genetic Comparisons of Epstein-Barr Virus Nuclear Antigen 3A, 3B, and 3C Homologues Encoded by the Rhesus Lymphocryptovirus. J. Virol. 74: 5921-5932 [Abstract] [Full Text]
Lee, S. P., Chan, A. T. C., Cheung, S.-T., Thomas, W. A., CroomCarter, D., Dawson, C. W., Tsai, C.-H., Leung, S.-F., Johnson, P. J., Huang, D. P. (2000). CTL Control of EBV in Nasopharyngeal Carcinoma (NPC): EBV-Specific CTL Responses in the Blood and Tumors of NPC Patients and the Antigen-Processing Function of the Tumor Cells. J. Immunol. 165: 573-582 [Abstract] [Full Text]
Caldwell, R. G., Brown, R. C., Longnecker, R. (2000). Epstein-Barr Virus LMP2A-Induced B-Cell Survival in Two Unique Classes of E{micro}LMP2A Transgenic Mice. J. Virol. 74: 1101-1113 [Abstract] [Full Text]
Liu, L., Usherwood, E. J., Blackman, M. A., Woodland, D. L. (1999). T-Cell Vaccination Alters the Course of Murine Herpesvirus 68 Infection and the Establishment of Viral Latency in Mice. J. Virol. 73: 9849-9857 [Abstract] [Full Text]
Charo, J., Ciupitu, A.-M. T., Le Chevalier de Preville, A., Trivedi, P., Klein, G., Hinkula, J., Kiessling, R. (1999). A Long-Term Memory Obtained by Genetic Immunization Results in Full Protection from a Mammary Adenocarcinoma Expressing an EBV Gene. J. Immunol. 163: 5913-5919 [Abstract] [Full Text]
Chu, P. G., Chang, K. L., Chen, W.-G., Chen, Y.-Y., Shibata, D., Hayashi, K., Bacchi, C., Bacchi, M., Weiss, L. M. (1999). Epstein-Barr Virus (EBV) Nuclear Antigen (EBNA)-4 Mutation in EBV-Associated Malignancies in Three Different Populations. Am. J. Pathol. 155: 941-947 [Abstract] [Full Text]
Tao, Q., Swinnen, L. J., Yang, J., Srivastava, G., Robertson, K. D., Ambinder, R. F. (1999). Methylation Status of the Epstein-Barr Virus Major Latent Promoter C in Iatrogenic B Cell Lymphoproliferative Disease : Application of PCR-Based Analysis. Am. J. Pathol. 155: 619-625 [Abstract] [Full Text]
Khatri, V. P., Baiocchi, R. A., Peng, R., Oberkircher, A. R., Dolce, J. M., Ward, P. M., Herzig, G. P., Caligiuri, M. A. (1999). Endogenous CD8+ T Cell Expansion During Regression of Monoclonal EBV-Associated Posttransplant Lymphoproliferative Disorder. J. Immunol. 163: 500-506 [Abstract] [Full Text]
Hollsberg, P. (1999). Mechanisms of T-Cell Activation by Human T-Cell Lymphotropic Virus Type I. Microbiol. Mol. Biol. Rev. 63: 308-333 [Abstract] [Full Text]
Chen, H., Smith, P., Ambinder, R. F., Hayward, S. D. (1999). Expression of Epstein-Barr Virus BamHI-A Rightward Transcripts in Latently Infected B Cells From Peripheral Blood. Blood 93: 3026-3032 [Abstract] [Full Text]
Khanna, R., Sherritt, M., Burrows, S. R. (1999). EBV Structural Antigens, gp350 and gp85, as Targets for Ex Vivo Virus-Specific CTL During Acute Infectious Mononucleosis: Potential Use of gp350/gp85 CTL Epitopes for Vaccine Design. J. Immunol. 162: 3063-3069 [Abstract] [Full Text]
Faulkner, G. C., Burrows, S. R., Khanna, R., Moss, D. J., Bird, A. G., Crawford, D. H. (1999). X-Linked Agammaglobulinemia Patients Are Not Infected with Epstein-Barr Virus: Implications for the Biology of the Virus. J. Virol. 73: 1555-1564 [Abstract] [Full Text]
Tan, L. C., Gudgeon, N., Annels, N. E., Hansasuta, P., O'Callaghan, C. A., Rowland-Jones, S., McMichael, A. J., Rickinson, A. B., Callan, M. F. C. (1999). A Re-Evaluation of the Frequency of CD8+ T Cells Specific for EBV in Healthy Virus Carriers. J. Immunol. 162: 1827-1835 [Abstract] [Full Text]
Redchenko, I. V., Rickinson, A. B. (1999). Accessing Epstein-Barr Virus-Specific T-Cell Memory with Peptide-Loaded Dendritic Cells. J. Virol. 73: 334-342 [Abstract] [Full Text]
Kalams, S. A., Walker, B. D. (1998). The Critical Need for CD4 Help in Maintaining Effective Cytotoxic T Lymphocyte Responses. JEM 188: 2199-2204 [Full Text]
Pepperl, S., Benninger-Doring, G., Modrow, S., Wolf, H., Jilg, W. (1998). Immediate-Early Transactivator Rta of Epstein-Barr Virus (EBV) Shows Multiple Epitopes Recognized by EBV-Specific Cytotoxic T Lymphocytes. J. Virol. 72: 8644-8649 [Abstract] [Full Text]
Bosshart, H., Jarrett, R. F. (1998). Deficient Major Histocompatibility Complex Class II Antigen Presentation in a Subset of Hodgkin's Disease Tumor Cells. Blood 92: 2252-2259 [Abstract] [Full Text]
Murray, P. G., Constandinou, C. M., Crocker, J., Young, L. S., Ambinder, R. F. (1998). Analysis of Major Histocompatibility Complex Class I, TAP Expression, and LMP2 Epitope Sequence in Epstein-Barr Virus-Positive Hodgkin's Disease. Blood 92: 2477-2483 [Abstract] [Full Text]
Kienzle, N., Sculley, T. B., Poulsen, L., Buck, M., Cross, S., Raab-Traub, N., Khanna, R. (1998). Identification of a Cytotoxic T-Lymphocyte Response to the Novel BARF0 Protein of Epstein-Barr Virus: a Critical Role for Antigen Expression. J. Virol. 72: 6614-6620 [Abstract] [Full Text]
Lee, S. P., Constandinou, C. M., Thomas, W. A., Croom-Carter, D., Blake, N. W., Murray, P. G., Crocker, J., Rickinson, A. B. (1998). Antigen Presenting Phenotype of Hodgkin Reed-Sternberg Cells: Analysis of the HLA Class I Processing Pathway and the Effects of Interleukin-10 on Epstein-Barr Virus-Specific Cytotoxic T-Cell Recognition. Blood 92: 1020-1030 [Abstract] [Full Text]
Gueguen, M., Patard, J.-J., Gaugler, B., Brasseur, F., Renauld, J.-C., Van Cangh, P. J., Boon, T., Van den Eynde, B. J. (1998). An Antigen Recognized by Autologous CTLs on a Human Bladder Carcinoma. J. Immunol. 160: 6188-6194 [Abstract] [Full Text]
Callan, M.F.C., Tan, L., Annels, N., Ogg, G.S., Wilson, J.D.K., O'Callaghan, C.A., Steven, N., McMichael, A.J., Rickinson, A.B. (1998). Direct Visualization of Antigen-specific CD8+T Cells during the Primary Immune Response to Epstein-Barr Virus In Vivo. JEM 187: 1395-1402 [Abstract] [Full Text]
Roskrow, M. A., Suzuki, N., Gan, Y.-j., Sixbey, J. W., Ng, C. Y.C., Kimbrough, S., Hudson, M., Brenner, M. K., Heslop, H. E., Rooney, C. M. (1998). Epstein-Barr Virus (EBV)-Specific Cytotoxic T Lymphocytes for the Treatment of Patients With EBV-Positive Relapsed Hodgkin's Disease. Blood 91: 2925-2934 [Abstract] [Full Text]
Kieff, E. (1998). Current Perspectives on the Molecular Pathogenesis of Virus-Induced Cancers in Human Immunodeficiency Virus Infection and Acquired Immunodeficiency Syndrome. J Natl Cancer Inst Monogr 1998: 7-14 [Abstract] [Full Text]
Thomson, S. A., Burrows, S. R., Misko, I. S., Moss, D. J., Coupar, B. E.�H., Khanna, R. (1998). Targeting a Polyepitope Protein Incorporating Multiple Class II-Restricted Viral Epitopes to the Secretory/Endocytic Pathway Facilitates Immune Recognition by CD4+ Cytotoxic T Lymphocytes: a Novel Approach to Vaccine Design. J. Virol. 72: 2246-2252 [Abstract] [Full Text]
Thomson, S. A., Sherritt, M. A., Medveczky, J., Elliott, S. L., Moss, D. J., Fernando, G. J. P., Brown, L. E., Suhrbier, A. (1998). Delivery of Multiple CD8 Cytotoxic T Cell Epitopes by DNA Vaccination. J. Immunol. 160: 1717-1723 [Abstract] [Full Text]
Low, H. P., Santos, M. A. M., Wizel, B., Tarleton, R. L. (1998). Amastigote Surface Proteins of Trypanosoma cruzi Are Targets for CD8+ CTL. J. Immunol. 160: 1817-1823 [Abstract] [Full Text]
Mukherjee, S., Trivedi, P., Dorfman, D. M., Klein, G., Townsend, A. (1998). Murine Cytotoxic T Lymphocytes Recognize an Epitope in an EBNA-1 Fragment, but Fail to Lyse EBNA-1-expressing Mouse Cells. JEM 187: 445-450 [Abstract] [Full Text]
Robertson, K. D., Ambinder, R. F. (1997). Methylation of the Epstein-Barr Virus Genome in Normal Lymphocytes. Blood 90: 4480-4484 [Abstract] [Full Text]
Levitskaya, J., Sharipo, A., Leonchiks, A., Ciechanover, A., Masucci, M. G. (1997). Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat domain of the Epstein-Barr virus nuclear antigen�1. Proc. Natl. Acad. Sci. USA 94: 12616-12621 [Abstract] [Full Text]
Niedobitek, G., Kremmer, E., Herbst, H., Whitehead, L., Dawson, C.W., Niedobitek, E., von Ostau, C., Rooney, N., Grasser, F.A., Young, L.S. (1997). Immunohistochemical Detection of the Epstein-Barr Virus-Encoded Latent Membrane Protein 2A in Hodgkin's Disease and Infectious Mononucleosis. Blood 90: 1664-1672 [Abstract] [Full Text]
Steven, N.M., Annels, N.E., Kumar, A., Leese, A.M., Kurilla, M.G., Rickinson, A.B. (1997). Immediate Early and Early Lytic Cycle Proteins Are Frequent Targets of the Epstein-Barr Virus-induced Cytotoxic T Cell Response. JEM 185: 1605-1618 [Abstract] [Full Text]
Prang, N. S., Hornef, M. W., Jager, M., Wagner, H. J., Wolf, H., Schwarzmann, F. M. (1997). Lytic Replication of Epstein-Barr Virus in the Peripheral Blood: Analysis of Viral Gene Expression in B Lymphocytes During Infectious Mononucleosis and in the Normal Carrier State. Blood 89: 1665-1677 [Abstract] [Full Text]
Liebowitz, D. (1995). Epstein-Barr Virus -- An Old Dog with New Tricks. NEJM 332: 55-57 [Full Text]
de Campos-Lima, P., Gavioli, R, Zhang, Q., Wallace, L., Dolcetti, R, Rowe, M, Rickinson, A., Masucci, M. (1993). HLA-A11 epitope loss isolates of Epstein-Barr virus from a highly A11+ population. Science 260: 98-100 [Abstract]
Tu, G., Kirchmaier, A. L., Liggitt, D., Liu, Y., Liu, S., Yu, W. H., Heath, T. D., Thor, A., Debs, R. J. (2000). Non-replicating Epstein-Barr Virus-based Plasmids Extend Gene Expression and Can Improve Gene Therapy in Vivo. J. Biol. Chem. 275: 30408-30416 [Abstract] [Full Text]
Tellam, J., Sherritt, M., Thomson, S., Tellam, R., Moss, D. J., Burrows, S. R., Wiertz, E., Khanna, R. (2001). Targeting of EBNA1 for Rapid Intracellular Degradation Overrides the Inhibitory Effects of the Gly-Ala Repeat Domain and Restores CD8+ T Cell Recognition. J. Biol. Chem. 276: 33353-33360 [Abstract] [Full Text]