Functional reconstitution of an immunoglobulin antigen receptor in T cells

doi:10.1084/jem.175.6.1669

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Functional reconstitution of an immunoglobulin antigen receptor in T cells

TE Costa, RR Franke, M Sanchez, Z Misulovin and MC Nussenzweig
Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.

Humoral immune responses are initiated by binding of antigen to the immunoglobulins (Igs) on the plasma membrane of B lymphocytes. On the cell surface, Ig forms a complex with several other proteins, two of which, MB-1 and B29, have been implicated in receptor assembly. We have reconstituted Ig receptor function in T lymphocytes by transfection of cloned receptor components. We found that efficient transport of IgM to the surface of T cells required coexpression of B29. Furthermore, IgM and B29 alone were sufficient to reconstitute antigen-specific signal transduction by Ig in the transfected T cells. Crosslinking of IgM with either antireceptor antibodies or antigen induced a calcium flux, phosphoinositol turnover, and interleukin secretion in T cells. These experiments establish a requirement for B29 in Ig receptor function, and suggest that the signaling apparatus of T and B cells is structurally homologous.
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