Continuous anti-interleukin 10 antibody administration depletes mice of Ly-1 B cells but not conventional B cells

doi:10.1084/jem.175.5.1213

This Article

	Full Text (PDF, 773K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ishida, H.
	Articles by Howard, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ishida, H.
	Articles by Howard, M.


Social Bookmarking

	What's this?

ARTICLES

Continuous anti-interleukin 10 antibody administration depletes mice of Ly-1 B cells but not conventional B cells

H Ishida, R Hastings, J Kearney and M Howard
DNAX Research Institute, Palo Alto, California 94304.

Ly-1 B cells have the distinctive property of continuous self- replenishment and, as we have shown previously, can be further distinguished from conventional B cells on the basis of greatly elevated constitutive and inducible production of the recently described cytokine interleukin 10 (IL-10). To test the possibility that IL-10 acts as either an autocrine or paracrine growth factor for Ly-1 B cells, we treated mice continuously from birth to 8 wk of age with a monoclonal rat IgM antibody that specifically neutralizes mouse IL-10. Mice treated in this way lacked peritoneal-resident Ly-1 B cells, contained greatly reduced serum immunoglobulin M levels, and were unable to generate significant in vivo antibody responses to intraperitoneal injections of alpha 1,3-dextran or phosphorylcholine, antigens for which specific B cells reside in the Ly-1 B cell subset. In contrast, conventional splenic B cells of anti-IL-10-treated mice were normal with respect to total numbers, phenotype, and in vitro responsiveness to B cell mitogens and the thymus-dependent antigen trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH). The mechanism of Ly- 1 B cell depletion appeared to be related to elevation of endogenous interferon gamma (IFN-gamma) levels in anti-IL-10-treated mice, since coadministration of neutralizing anti-IFN-gamma antibodies substantially restored the number of peritoneal-resident Ly-1 B cells in these mice. These results implicate IL-10 as a regulator of Ly-1 B cell development, and identify a procedure to specifically deplete Ly-1 B cells, thereby allowing further evaluation of the role of these cells in the immune system.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ostrowski, M., Vermeulen, M., Zabal, O., Zamorano, P. I., Sadir, A. M., Geffner, J. R., Lopez, O. J. (2007). The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9+ B Lymphocytes. J. Virol. 81: 9357-9367 [Abstract] [Full Text]
Taneichi, M., Ishida, H., Kajino, K., Ogasawara, K., Tanaka, Y., Kasai, M., Mori, M., Nishida, M., Yamamura, H., Mizuguchi, J., Uchida, T. (2006). Antigen Chemically Coupled to the Surface of Liposomes Are Cross-Presented to CD8+ T Cells and Induce Potent Antitumor Immunity. J. Immunol. 177: 2324-2330 [Abstract] [Full Text]
Binder, C. J., Shaw, P. X., Chang, M.-K., Boullier, A., Hartvigsen, K., Horkko, S., Miller, Y. I., Woelkers, D. A., Corr, M., Witztum, J. L. (2005). Thematic review series: The Immune System and Atherogenesis. The role of natural antibodies in atherogenesis. J. Lipid Res. 46: 1353-1363 [Abstract] [Full Text]
Rahimi, A. A. R., Gee, K., Mishra, S., Lim, W., Kumar, A. (2005). STAT-1 Mediates the Stimulatory Effect of IL-10 on CD14 Expression in Human Monocytic Cells. J. Immunol. 174: 7823-7832 [Abstract] [Full Text]
Tumang, J. R., Frances, R., Yeo, S. G., Rothstein, T. L. (2005). Cutting Edge: Spontaneously Ig-Secreting B-1 Cells Violate the Accepted Paradigm for Expression of Differentiation-Associated Transcription Factors. J. Immunol. 174: 3173-3177 [Abstract] [Full Text]
Moll, T., Martinez-Soria, E., Santiago-Raber, M.-L., Amano, H., Pihlgren-Bosch, M., Marinkovic, D., Izui, S. (2005). Differential Activation of Anti-Erythrocyte and Anti-DNA Autoreactive B Lymphocytes by the Yaa Mutation. J. Immunol. 174: 702-709 [Abstract] [Full Text]
Mori, S., Sakamoto, A., Yamashita, K., Fujimura, L., Arima, M., Hatano, M., Miyazaki, M., Tokuhisa, T. (2004). Effect of c-fos overexpression on development and proliferation of peritoneal B cells. Int Immunol 16: 1477-1486 [Abstract] [Full Text]
Yurino, H., Ishikawa, S., Sato, T., Akadegawa, K., Ito, T., Ueha, S., Inadera, H., Matsushima, K. (2004). Endocrine Disruptors (Environmental Estrogens) Enhance Autoantibody Production by B1 Cells. Toxicol Sci 81: 139-147 [Abstract] [Full Text]
Brandlein, S., Pohle, T., Ruoff, N., Wozniak, E., Muller-Hermelink, H.-K., Vollmers, H. P. (2003). Natural IgM Antibodies and Immunosurveillance Mechanisms against Epithelial Cancer Cells in Humans. Cancer Res. 63: 7995-8005 [Abstract] [Full Text]
Whyburn, L. R., Halcomb, K. E., Contreras, C. M., Lowell, C. A., Witte, O. N., Satterthwaite, A. B. (2003). Reduced Dosage of Bruton's Tyrosine Kinase Uncouples B Cell Hyperresponsiveness from Autoimmunity in lyn-/- Mice. J. Immunol. 171: 1850-1858 [Abstract] [Full Text]
Balabanian, K., Couderc, J., Bouchet-Delbos, L., Amara, A., Berrebi, D., Foussat, A., Baleux, F., Portier, A., Durand-Gasselin, I., Coffman, R. L., Galanaud, P., Peuchmaur, M., Emilie, D. (2003). Role of the Chemokine Stromal Cell-Derived Factor 1 in Autoantibody Production and Nephritis in Murine Lupus. J. Immunol. 170: 3392-3400 [Abstract] [Full Text]
Gary-Gouy, H., Harriague, J., Bismuth, G., Platzer, C., Schmitt, C., Dalloul, A. H. (2002). Human CD5 promotes B-cell survival through stimulation of autocrine IL-10 production. Blood 100: 4537-4543 [Abstract] [Full Text]
Taneichi, M., Naito, S., Kato, H., Tanaka, Y., Mori, M., Nakano, Y., Yamamura, H., Ishida, H., Komuro, K., Uchida, T. (2002). T Cell-Independent Regulation of IgE Antibody Production Induced by Surface-Linked Liposomal Antigen. J. Immunol. 169: 4246-4252 [Abstract] [Full Text]
Rothstein, T. L. (2002). Two B-1 or Not To Be One. J. Immunol. 168: 4257-4261 [Abstract] [Full Text]
Tezuka, H., Imai, S., Muto, R., Furuhashi, Y., Fujita, K. (2002). Recombinant Dirofilaria immitis Polyprotein That Stimulates Murine B Cells To Produce Nonspecific Polyclonal Immunoglobulin E Antibody. Infect. Immun. 70: 1235-1244 [Abstract] [Full Text]
Balabanian, K., Foussat, A., Bouchet-Delbos, L., Couderc, J., Krzysiek, R., Amara, A., Baleux, F., Portier, A., Galanaud, P., Emilie, D. (2002). Interleukin-10 modulates the sensitivity of peritoneal B lymphocytes to chemokines with opposite effects on stromal cell-derived factor-1 and B-lymphocyte chemoattractant. Blood 99: 427-436 [Abstract] [Full Text]
Bleesing, J. J. H., Brown, M. R., Straus, S. E., Dale, J. K., Siegel, R. M., Johnson, M., Lenardo, M. J., Puck, J. M., Fleisher, T. A. (2001). Immunophenotypic profiles in families with autoimmune lymphoproliferative syndrome. Blood 98: 2466-2473 [Abstract] [Full Text]
Lopatin, U., Yao, X., Williams, R. K., Bleesing, J. J. H., Dale, J. K., Wong, D., Teruya-Feldstein, J., Fritz, S., Morrow, M. R., Fuss, I., Sneller, M. C., Raffeld, M., Fleisher, T. A., Puck, J. M., Strober, W., Jaffe, E. S., Straus, S. E. (2001). Increases in circulating and lymphoid tissue interleukin-10 in autoimmune lymphoproliferative syndrome are associated with disease expression. Blood 97: 3161-3170 [Abstract] [Full Text]
Sakiyama, T., Ikuta, K., Nisitani, S., Takatsu, K., Honjo, T. (1999). Requirement of IL-5 for induction of autoimmune hemolytic anemia in anti-red blood cell autoantibody transgenic mice. Int Immunol 11: 995-1000 [Abstract] [Full Text]
Boes, M., Esau, C., Fischer, M. B., Schmidt, T., Carroll, M., Chen, J. (1998). Enhanced B-1 Cell Development, But Impaired IgG Antibody Responses in Mice Deficient in Secreted IgM. J. Immunol. 160: 4776-4787 [Abstract] [Full Text]
Chevallier, N., Berthelemy, M., Le Rhun, D., Laine, V., Levy, D., Schwartz-Cornil, I. (1998). Bovine Leukemia Virus-Induced Lymphocytosis and Increased Cell Survival Mainly Involve the CD11b+ B-Lymphocyte Subset in Sheep. J. Virol. 72: 4413-4420 [Abstract] [Full Text]
Satterthwaite, A. B., Cheroutre, H., Khan, W. N., Sideras, P., Witte, O. N. (1997). Btk dosage determines sensitivity to B cell antigen receptor�cross-linking. Proc. Natl. Acad. Sci. USA 94: 13152-13157 [Abstract] [Full Text]
Tarakhovsky, A. (1997). Bar Mitzvah for B-1 Cells: How Will They Grow Up?. JEM 185: 981-984 [Full Text]
Ma, W., Lim, W., Gee, K., Aucoin, S., Nandan, D., Kozlowski, M., Diaz-Mitoma, F., Kumar, A. (2001). The p38 Mitogen-activated Kinase Pathway Regulates the Human Interleukin-10 Promoter via the Activation of Sp1 Transcription Factor in Lipopolysaccharide-stimulated Human Macrophages. J. Biol. Chem. 276: 13664-13674 [Abstract] [Full Text]