Molecular analysis of the induction of immunoglobulin E synthesis in human B cells by interleukin 4 and engagement of CD40 antigen

doi:10.1084/jem.175.1.289

This Article

	Full Text (PDF, 617K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Shapira, S. K.
	Articles by Geha, R. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Shapira, S. K.
	Articles by Geha, R. S.


Social Bookmarking

	What's this?

ARTICLES

Molecular analysis of the induction of immunoglobulin E synthesis in human B cells by interleukin 4 and engagement of CD40 antigen

SK Shapira, D Vercelli, HH Jabara, SM Fu and RS Geha
Division of Immunology, Children's Hospital, Boston, Massachusetts 02115.

The molecular events leading to immunoglobulin E (IgE) synthesis in human sIgE- B cells stimulated with interleukin 4 (IL-4) and anti-CD40 monoclonal antibody (mAb) 626.1 were analyzed. Anti-CD40 mAb increased the levels of IL-4-induced germline C epsilon transcripts and induced the production of mature C epsilon mRNA. These effects were dependent on the presence of IL-4. Nested primer PCR revealed deletional switch recombination occurring only in B cell stimulated with both IL-4 and anti-CD40 mAb. DNA sequence analysis of switch fragments showed direct S mu/S epsilon joining, without the deletions or duplications within S mu often found in B cells stimulated with IL-4 and Epstein-Barr virus. Analysis of the switch junction map sites showed "hot spots" for recombination within S mu, but not within S epsilon. These findings indicate that IL-4 provides a signal to B cells to induce germline C epsilon transcription and concurrent CD40 engagement induces S mu/S epsilon deletional switch recombination, production of mature C epsilon mRNA, and IgE synthesis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Avery, D. T., Ma, C. S., Bryant, V. L., Santner-Nanan, B., Nanan, R., Wong, M., Fulcher, D. A., Cook, M. C., Tangye, S. G. (2008). STAT3 is required for IL-21-induced secretion of IgE from human naive B cells. Blood 112: 1784-1793 [Abstract] [Full Text]
Yamada, T., Zhu, D., Zhang, K., Saxon, A. (2003). Inhibition of Interleukin-4-induced Class Switch Recombination by a Human Immunoglobulin Fc{gamma}-Fc{epsilon} Chimeric Protein. J. Biol. Chem. 278: 32818-32824 [Abstract] [Full Text]
Yamada, T., Zhu, D., Saxon, A., Zhang, K. (2002). CD45 Controls Interleukin-4-mediated IgE Class Switch Recombination in Human B Cells through Its Function as a Janus Kinase Phosphatase. J. Biol. Chem. 277: 28830-28835 [Abstract] [Full Text]
Nakamura, H., Luster, A. D., Tateno, H., Jedrzkiewicz, S., Tamura, G., Haley, K. J., Garcia-Zepeda, E. A., Yamaguchi, K., Lilly, C. M. (2001). IL-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 281: L1288-L1302 [Abstract] [Full Text]
VERCELLI, D. (2000). One Cytokine, Two Isotypes . A Trojan Horse, Pandora's Box, and an Evolving Paradigm. Am. J. Respir. Crit. Care Med. 162: S86-90 [Full Text]
Jalukar, S. V., Hostager, B. S., Bishop, G. A. (2000). Characterization of the Roles of TNF Receptor-Associated Factor 6 in CD40-Mediated B Lymphocyte Effector Functions. J. Immunol. 164: 623-630 [Abstract] [Full Text]
Hill, S., Herlaar, E., Le Cardinal, A., van Heeke, G., Nicklin, P. (1999). Homologous Human and Murine Antisense Oligonucleotides Targeting Stat6 . Functional Effects on Germline Cepsilon Transcript. Am. J. Respir. Cell Mol. Bio. 21: 728-737 [Abstract] [Full Text]
Revy, P., Hivroz, C., Andreu, G., Graber, P., Martinache, C., Fischer, A., Durandy, A. (1999). Activation of the Janus Kinase 3-STAT5a Pathway After CD40 Triggering of Human Monocytes But Not of Resting B Cells. J. Immunol. 163: 787-793 [Abstract] [Full Text]
Henriquez, N. V., Floettmann, E., Salmon, M., Rowe, M., Rickinson, A. B. (1999). Differential Responses to CD40 Ligation Among Burkitt Lymphoma Lines That Are Uniformly Responsive to Epstein-Barr Virus Latent Membrane Protein 1. J. Immunol. 162: 3298-3307 [Abstract] [Full Text]
Nagumo, H., Agematsu, K., Shinozaki, K., Hokibara, S., Ito, S., Takamoto, M., Nikaido, T., Yasui, K., Uehara, Y., Yachie, A., Komiyama, A. (1998). CD27/CD70 Interaction Augments IgE Secretion by Promoting the Differentiation of Memory B Cells into Plasma Cells. J. Immunol. 161: 6496-6502 [Abstract] [Full Text]
Jabara, H. H., Buckley, R. H., Roberts, J. L., Lefranc, G., Loiselet, J., Khalil, G., Geha, R. S. (1998). Role of JAK3 in CD40-Mediated Signaling. Blood 92: 2435-2440 [Abstract] [Full Text]
Linehan, L. A., Warren, W. D., Thompson, P. A., Grusby, M. J., Berton, M. T. (1998). STAT6 Is Required for IL-4-Induced Germline Ig Gene Transcription and Switch Recombination. J. Immunol. 161: 302-310 [Abstract] [Full Text]
Massari, M. E., Rivera, R. R., Voland, J. R., Quong, M. W, Breit, T. M., van Dongen, J. J.�M., de Smit, O., Murre, C. (1998). Characterization of ABF-1, a Novel Basic Helix-Loop-Helix Transcription Factor Expressed in Activated B Lymphocytes. Mol. Cell. Biol. 18: 3130-3139 [Abstract] [Full Text]
Purkerson, J. M., Parker, D. C. (1998). Differential Coupling of Membrane Ig and CD40 to the Extracellularly Regulated Kinase Signaling Pathway. J. Immunol. 160: 2121-2129 [Abstract] [Full Text]