A major T cell antigen of Mycobacterium leprae is a 10-kD heat-shock cognate protein

doi:10.1084/jem.175.1.275

This Article

	Full Text (PDF, 2694K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Mehra, V.
	Articles by Modlin, R. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Mehra, V.
	Articles by Modlin, R. L.


Social Bookmarking

	What's this?

ARTICLES

A major T cell antigen of Mycobacterium leprae is a 10-kD heat-shock cognate protein

V Mehra, BR Bloom, AC Bajardi, CL Grisso, PA Sieling, D Alland, J Convit, XD Fan, SW Hunter, PJ Brennan, and RL Modlin
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461.

Several mycobacterial antigens, identified by monoclonal antibodies and patient sera, have been found to be homologous to stress or heat-shock proteins (hsp) defined in Escherichia coli and yeast. A major antigen recognized by most Mycobacterium leprae-reactive human T cell lines and cell wall-reactive T cell clones is a 10-kD protein that has now been cloned and sequenced. The predicted amino acid sequence of this protein is 44% homologous to the hsp 10 (GroES) of E. coli. The purified native and recombinant 10-kD protein was found to be a stronger stimulator of peripheral blood T cell proliferation than other native and recombinant M. leprae proteins tested. The degree of reactivity paralleled the response to intact M. leprae throughout the spectrum of leprosy. Limiting-dilution analysis of peripheral blood lymphocytes from a patient contact and a tuberculoid patient indicated that approximately one third of M. leprae-reactive T cell precursors responded to the 10- kD antigen. T cell lines derived from lepromin skin tests were strongly responsive to the 10-kD protein. T cell clones reactive to both the purified native and recombinant 10-kD antigens recognized M. leprae- specific epitopes as well as epitopes crossreactive with the cognate antigen of M. tuberculosis. Further, the purified hsp 10 elicited strong delayed-type hypersensitivity reactions in guinea pigs sensitized to M. leprae. The strong T cell responses against the M. leprae 10-kD protein suggest a role for this heat-shock cognate protein in the protective/resistant responses to infection.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Sieling, P. A., Hill, P. J., Dobos, K. M., Brookman, K., Kuhlman, A. M., Fabri, M., Krutzik, S. R., Rea, T. H., Heaslip, D. G., Belisle, J. T., Modlin, R. L. (2008). Conserved Mycobacterial Lipoglycoproteins Activate TLR2 but Also Require Glycosylation for MHC Class II-Restricted T Cell Activation. J. Immunol. 180: 5833-5842 [Abstract] [Full Text]
Lee, D. J., Sieling, P. A., Ochoa, M. T., Krutzik, S. R., Guo, B., Hernandez, M., Rea, T. H., Cheng, G., Colonna, M., Modlin, R. L. (2007). LILRA2 Activation Inhibits Dendritic Cell Differentiation and Antigen Presentation to T Cells. J. Immunol. 179: 8128-8136 [Abstract] [Full Text]
Groathouse, N. A., Amin, A., Marques, M. A. M., Spencer, J. S., Gelber, R., Knudson, D. L., Belisle, J. T., Brennan, P. J., Slayden, R. A. (2006). Use of Protein Microarrays To Define the Humoral Immune Response in Leprosy Patients and Identification of Disease-State-Specific Antigenic Profiles. Infect. Immun. 74: 6458-6466 [Abstract] [Full Text]
Quiroga, M. F., Pasquinelli, V., Martinez, G. J., Jurado, J. O., Zorrilla, L. C., Musella, R. M., Abbate, E., Sieling, P. A., Garcia, V. E. (2006). Inducible Costimulator: A Modulator of IFN-{gamma} Production in Human Tuberculosis. J. Immunol. 176: 5965-5974 [Abstract] [Full Text]
Scollard, D. M., Adams, L. B., Gillis, T. P., Krahenbuhl, J. L., Truman, R. W., Williams, D. L. (2006). The Continuing Challenges of Leprosy. Clin. Microbiol. Rev. 19: 338-381 [Abstract] [Full Text]
Sieling, P. A., Torrelles, J. B., Stenger, S., Chung, W., Burdick, A. E., Rea, T. H., Brennan, P. J., Belisle, J. T., Porcelli, S. A., Modlin, R. L. (2005). The Human CD1-Restricted T Cell Repertoire Is Limited to Cross-Reactive Antigens: Implications for Host Responses against Immunologically Related Pathogens. J. Immunol. 174: 2637-2644 [Abstract] [Full Text]
Quiroga, M. F., Martinez, G. J., Pasquinelli, V., Costas, M. A., Bracco, M. M., Malbran, A., Olivares, L. M., Sieling, P. A., Garcia, V. E. (2004). Activation of Signaling Lymphocytic Activation Molecule Triggers a Signaling Cascade That Enhances Th1 Responses in Human Intracellular Infection. J. Immunol. 173: 4120-4129 [Abstract] [Full Text]
Pasquinelli, V., Quiroga, M. F., Martinez, G. J., Zorrilla, L. C., Musella, R. M., Bracco, M. M., Belmonte, L., Malbran, A., Fainboim, L., Sieling, P. A., Garcia, V. E. (2004). Expression of Signaling Lymphocytic Activation Molecule- Associated Protein Interrupts IFN-{gamma} Production in Human Tuberculosis. J. Immunol. 172: 1177-1185 [Abstract] [Full Text]
Sieling, P. A., Chung, W., Duong, B. T., Godowski, P. J., Modlin, R. L. (2003). Toll-Like Receptor 2 Ligands as Adjuvants for Human Th1 Responses. J. Immunol. 170: 194-200 [Abstract] [Full Text]
Spencer, J. S., Marques, M. A. M., Lima, M. C. B. S., Junqueira-Kipnis, A. P., Gregory, B. C., Truman, R. W., Brennan, P. J. (2002). Antigenic Specificity of the Mycobacterium leprae Homologue of ESAT-6. Infect. Immun. 70: 1010-1013 [Abstract] [Full Text]
Garcia, V. E., Quiroga, M. F., Ochoa, M. T., Ochoa, L., Pasquinelli, V., Fainboim, L., Olivares, L. M., Valdez, R., Sordelli, D. O., Aversa, G., Modlin, R. L., Sieling, P. A. (2001). Signaling Lymphocytic Activation Molecule Expression and Regulation in Human Intracellular Infection Correlate with Th1 Cytokine Patterns. J. Immunol. 167: 5719-5724 [Abstract] [Full Text]
Taneja, B., Mande, S. C. (2001). Metal ions modulate the plastic nature of Mycobacterium tuberculosis chaperonin-10. Protein Eng Des Sel 14: 391-395 [Abstract] [Full Text]
Macfarlane, A., Mondragon-Gonzalez, R., Vega-Lopez, F., Wieles, B., de Pena, J., Rodriguez, O., Suarez y de la Torre, R., de Vries, R. R. P., Ottenhoff, T. H. M., Dockrell, H. M. (2001). Presence of Human T-Cell Responses to the Mycobacterium leprae 45-Kilodalton Antigen Reflects Infection with or Exposure to M. leprae. CVI 8: 604-611 [Abstract] [Full Text]
Yamauchi, P. S., Bleharski, J. R., Uyemura, K., Kim, J., Sieling, P. A., Miller, A., Brightbill, H., Schlienger, K., Rea, T. H., Modlin, R. L. (2000). A Role for CD40-CD40 Ligand Interactions in the Generation of Type 1 Cytokine Responses in Human Leprosy. J. Immunol. 165: 1506-1512 [Abstract] [Full Text]
Sieling, P. A., Ochoa, M.-T., Jullien, D., Leslie, D. S., Sabet, S., Rosat, J.-P., Burdick, A. E., Rea, T. H., Brenner, M. B., Porcelli, S. A., Modlin, R. L. (2000). Evidence for Human CD4+ T Cells in the CD1-Restricted Repertoire: Derivation of Mycobacteria-Reactive T Cells from Leprosy Lesions. J. Immunol. 164: 4790-4796 [Abstract] [Full Text]
Skjot, R. L. V., Oettinger, T., Rosenkrands, I., Ravn, P., Brock, I., Jacobsen, S., Andersen, P. (2000). Comparative Evaluation of Low-Molecular-Mass Proteins from Mycobacterium tuberculosis Identifies Members of the ESAT-6 Family as Immunodominant T-Cell Antigens. Infect. Immun. 68: 214-220 [Abstract] [Full Text]
LaVerda, D., Albanese, L. N., Ruther, P. E., Morrison, S. G., Morrison, R. P., Ault, K. A., Byrne, G. I. (2000). Seroreactivity to Chlamydia trachomatis Hsp10 Correlates with Severity of Human Genital Tract Disease. Infect. Immun. 68: 303-309 [Abstract] [Full Text]
Rosenkrands, I., Weldingh, K., Ravn, P., Brandt, L., Hojrup, P., Rasmussen, P. B., Coates, A. R., Singh, M., Mascagni, P., Andersen, P. (1999). Differential T-Cell Recognition of Native and Recombinant Mycobacterium tuberculosis GroES. Infect. Immun. 67: 5552-5558 [Abstract] [Full Text]
Thole, J. E. R., Janson, A. A. M., Cornelisse, Y., Schreuder, G. M. T., Wieles, B., Naafs, B., de Vries, R. R. P., Ottenhoff, T. H. M. (1999). HLA-Class II-Associated Control of Antigen Recognition by T Cells in Leprosy: A Prominent Role for the 30/31-kDa Antigens. J. Immunol. 162: 6912-6918 [Abstract] [Full Text]
Garcia, V. E., Uyemura, K., Sieling, P. A., Ochoa, M. T., Morita, C. T., Okamura, H., Kurimoto, M., Rea, T. H., Modlin, R. L. (1999). IL-18 Promotes Type 1 Cytokine Production from NK Cells and T Cells in Human Intracellular Infection. J. Immunol. 162: 6114-6121 [Abstract] [Full Text]
Chua-Intra, B., Peerapakorn, S., Davey, N., Jurcevic, S., Busson, M., Vordermeier, H. M., Pirayavaraporn, C., Ivanyi, J. (1998). T-Cell Recognition of Mycobacterial GroES Peptides in Thai Leprosy Patients and Contacts. Infect. Immun. 66: 4903-4909 [Abstract] [Full Text]
Campbell, D. J., Shastri, N. (1998). Bacterial Surface Proteins Recognized by CD4+ T Cells During Murine Infection with Listeria monocytogenes. J. Immunol. 161: 2339-2347 [Abstract] [Full Text]
Fossati, G., Lucietto, P., Giuliani, P., Coates, A. R., Harding, S., C�lfen, H., Legname, G., Chan, E., Zaliani, A., Mascagni, P. (1995). Mycobacterium tuberculosis Chaperonin 10 Forms Stable Tetrameric and Heptameric Structures. J. Biol. Chem. 270: 26159-26167 [Abstract] [Full Text]