Antigen-pulsed dendritic cells can efficiently induce an antibody response in vivo

doi:10.1084/jem.175.1.15

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Antigen-pulsed dendritic cells can efficiently induce an antibody response in vivo

T Sornasse, V Flamand, G De Becker, H Bazin, F Tielemans, K Thielemans, J Urbain, O Leo and M Moser
Departement de Biologie Moleculaire, Universite Libre de Bruxelles, Belgium.

The aim of this study was to develop an immunization procedure avoiding external adjuvant. Data are presented showing that syngeneic dendritic cells (DC), which have been pulsed in vitro with antigen, induce a strong antibody response in mice. By contrast, antigen (Ag)-pulsed low- density B cells, although equally able to induce interleukin 2 secretion by an Ag-specific T cell hybridoma in vitro, only weakly prime the mice in vivo. Moreover, we show that the injection of Ag- pulsed DC induces the synthesis of isotypes similar to the immunoglobulin classes detected after immunization with the same Ag in complete Freund's adjuvant. Importantly, high amounts of IgG2a antibodies are produced, suggesting that T helper type 1 cells are activated. Collectively, these data indicate that DC can initiate a primary humoral response and that they may be used as physiological adjuvant in vivo.
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Hart, D. N.J. (1997). Dendritic Cells: Unique Leukocyte Populations Which Control the Primary Immune Response. Blood 90: 3245-3287 [Full Text]
Caux, C., Massacrier, C., Vanbervliet, B., Dubois, B., Durand, I., Cella, M., Lanzavecchia, A., Banchereau, J. (1997). CD34+ Hematopoietic Progenitors From Human Cord Blood Differentiate Along Two Independent Dendritic Cell Pathways in Response to Granulocyte-Macrophage Colony-Stimulating Factor Plus Tumor Necrosis Factor alpha : II. Functional Analysis. Blood 90: 1458-1470 [Abstract] [Full Text]
Fayette, J., Dubois, B., Vandenabeele, S., Bridon, J.-M., Vanbervliet, B., Durand, I., Banchereau, J., Caux, C., Briere, F. (1997). Human Dendritic Cells Skew Isotype Switching of CD40-activated Naive B Cells towards IgA1 and IgA2. JEM 185: 1909-1918 [Abstract] [Full Text]
Dubois, B., Vanbervliet, B., Fayette, J., Massacrier, C., Kooten, C. V., Briere, F., Banchereau, J., Caux, C. (1997). Dendritic Cells Enhance Growth and Differentiation of CD40-activated B Lymphocytes. JEM 185: 941-952 [Abstract] [Full Text]
Clark, E. A. (1997). Regulation of B Lymphocytes by Dendritic Cells. JEM 185: 801-804 [Full Text]
Wolf, S. D., Dittel, B. N., Hardardottir, F., Janeway, C. A. Jr. (1996). Experimental Autoimmune Encephalomyelitis Induction in Genetically B Cell-deficient Mice. JEM 184: 2271-2278 [Abstract] [Full Text]