Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility

doi:10.1084/jem.174.6.1347

This Article

	Full Text (PDF, 1030K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Miyake, M.
	Articles by Ikeyama, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Miyake, M.
	Articles by Ikeyama, S.


Social Bookmarking

	What's this?

ARTICLES

Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility

M Miyake, M Koyama, M Seno and S Ikeyama
Department of Thoracic Surgery, Kitano Hospital, Tazuke Kofukai Medical Research Institute, Osaka, Japan.

A murine monoclonal antibody (M31-15) was identified using the penetration-inhibiting assay of a human lung adenocarcinoma cell line (MAC10) and remarkably inhibited the phagokinetic tract motility of various cancer cell lines. The antigen, motility-related protein (MRP- 1), recognized by M31-15, was 25- and 28-kD proteins, and M31-15 was used to isolate a cDNA clone from a human breast carcinoma cDNA library. Sequence analysis revealed that MRP-1 had strong similarity with a B cell surface antigen (CD37), a melanoma-associated antigen (ME491), the target of an antiproliferative antibody (TAPA-1), a human tumor-associated antigen (CO-029), and the Sm23 antigen of the trematode parasite Schistosoma mansoni.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Nakazawa, Y., Sato, S., Naito, M., Kato, Y., Mishima, K., Arai, H., Tsuruo, T., Fujita, N. (2008). Tetraspanin family member CD9 inhibits Aggrus/podoplanin-induced platelet aggregation and suppresses pulmonary metastasis. Blood 112: 1730-1739 [Abstract] [Full Text]
Wang, J.-C., Begin, L. R., Berube, N. G., Chevalier, S., Aprikian, A. G., Gourdeau, H., Chevrette, M. (2007). Down-Regulation of CD9 Expression during Prostate Carcinoma Progression Is Associated with CD9 mRNA Modifications. Clin. Cancer Res. 13: 2354-2361 [Abstract] [Full Text]
Takeda, T., Hattori, N., Tokuhara, T., Nishimura, Y., Yokoyama, M., Miyake, M. (2007). Adenoviral Transduction of MRP-1/CD9 and KAI1/CD82 Inhibits Lymph Node Metastasis in Orthotopic Lung Cancer Model. Cancer Res. 67: 1744-1749 [Abstract] [Full Text]
Qi, J. C., Wang, J., Mandadi, S., Tanaka, K., Roufogalis, B. D., Madigan, M. C., Lai, K., Yan, F., Chong, B. H., Stevens, R. L., Krilis, S. A. (2006). Human and mouse mast cells use the tetraspanin CD9 as an alternate interleukin-16 receptor. Blood 107: 135-142 [Abstract] [Full Text]
Mitsuzuka, K., Handa, K., Satoh, M., Arai, Y., Hakomori, S. (2005). A Specific Microdomain ("Glycosynapse 3") Controls Phenotypic Conversion and Reversion of Bladder Cancer Cells through GM3-mediated Interaction of {alpha}3{beta}1 Integrin with CD9. J. Biol. Chem. 280: 35545-35553 [Abstract] [Full Text]
Cariappa, A., Shoham, T., Liu, H., Levy, S., Boucheix, C., Pillai, S. (2005). The CD9 Tetraspanin Is Not Required for the Development of Peripheral B Cells or for Humoral Immunity. J. Immunol. 175: 2925-2930 [Abstract] [Full Text]
Toledo, M. S., Suzuki, E., Handa, K., Hakomori, S. (2004). Cell Growth Regulation through GM3-enriched Microdomain (Glycosynapse) in Human Lung Embryonal Fibroblast WI38 and Its Oncogenic Transformant VA13. J. Biol. Chem. 279: 34655-34664 [Abstract] [Full Text]
Gutierrez-Lopez, M. D., Ovalle, S., Yanez-Mo, M., Sanchez-Sanchez, N., Rubinstein, E., Olmo, N., Lizarbe, M. A., Sanchez-Madrid, F., Cabanas, C. (2003). A Functionally Relevant Conformational Epitope on the CD9 Tetraspanin Depends on the Association with Activated beta 1 Integrin. J. Biol. Chem. 278: 208-218 [Abstract] [Full Text]
Fritzsching, B., Schwer, B., Kartenbeck, J., Pedal, A., Horejsi, V., Ott, M. (2002). Release and Intercellular Transfer of Cell Surface CD81 Via Microparticles. J. Immunol. 169: 5531-5537 [Abstract] [Full Text]
Kawakami, Y., Kawakami, K., Steelant, W. F. A., Ono, M., Baek, R. C., Handa, K., Withers, D. A., Hakomori, S. (2002). Tetraspanin CD9 Is a "Proteolipid," and Its Interaction with alpha 3 Integrin in Microdomain Is Promoted by GM3 Ganglioside, Leading to Inhibition of Laminin-5-dependent Cell Motility. J. Biol. Chem. 277: 34349-34358 [Abstract] [Full Text]
Watanabe, R., Ohyama, C., Aoki, H., Takahashi, T., Satoh, M., Saito, S., Hoshi, S., Ishii, A., Saito, M., Arai, Y. (2002). Ganglioside GM3 Overexpression Induces Apoptosis and Reduces Malignant Potential in Murine Bladder Cancer. Cancer Res. 62: 3850-3854 [Abstract] [Full Text]
Hakomori, S.-i. (2002). The glycosynapse. Proc. Natl. Acad. Sci. USA 10.1073/pnas.012540899v1 [Abstract] [Full Text]
Nakamura, Y., Handa, K., Iwamoto, R., Tsukamoto, T., Takahasi, M., Mekada, E. (2001). Immunohistochemical Distribution of CD9, Heparin Binding Epidermal Growth Factor-like Growth Factor, and Integrin {{alpha}}3{beta}1 in Normal Human Tissues. J. Histochem. Cytochem. 49: 439-444 [Abstract] [Full Text]
Berditchevski, F. (2001). Complexes of tetraspanins with integrins: more than meets the eye. J. Cell Sci. 114: 4143-4151 [Abstract] [Full Text]
Shallal, S., Kornbluth, J. (2000). CD9 expression enhances the susceptibility of myeloma cell lines to cell-mediated cytolysis. Blood 96: 224-233 [Abstract] [Full Text]
Stipp, C., Hemler, M. (2000). Transmembrane-4-superfamily proteins CD151 and CD81 associate with alpha 3 beta 1 integrin, and selectively contribute to alpha 3 beta 1-dependent neurite outgrowth. J. Cell Sci. 113: 1871-1882 [Abstract]
Tachibana, I., Hemler, M. E. (1999). Role of Transmembrane 4 Superfamily (Tm4sf) Proteins Cd9 and Cd81 in Muscle Cell Fusion and Myotube Maintenance. JCB 146: 893-904 [Abstract] [Full Text]
Testa, J. E., Brooks, P. C., Lin, J.-M., Quigley, J. P. (1999). Eukaryotic Expression Cloning with an Antimetastatic Monoclonal Antibody Identifies a Tetraspanin (PETA-3/CD151) as an Effector of Human TumorCell Migration and Metastasis. Cancer Res. 59: 3812-3820 [Abstract] [Full Text]
Berditchevski, F., Odintsova, E. (1999). Characterization of Integrin-Tetraspanin Adhesion Complexes: Role of Tetraspanins in Integrin Signaling. JCB 146: 477-492 [Abstract] [Full Text]
Ono, M., Handa, K., Withers, D. A., Hakomori, S.-i. (1999). Motility Inhibition and Apoptosis Are Induced by Metastasis-suppressing Gene Product CD82 and Its Analogue CD9, with Concurrent Glycosylation. Cancer Res. 59: 2335-2339 [Abstract] [Full Text]
Hirano, T., Higuchi, T., Ueda, M., Inoue, T., Kataoka, N., Maeda, M., Fujiwara, H., Fujii, S. (1999). CD9 is expressed in extravillous trophoblasts in association with integrin {alpha}3 and integrin {alpha}5. Mol Hum Reprod 5: 162-167 [Abstract] [Full Text]
Hirano, T., Higuchi, T., Katsuragawa, H., Inoue, T., Kataoka, N., Park, K. R., Ueda, M., Maeda, M., Fujiwara, H., Fujii, S. (1999). CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line, BeWo cells. Mol Hum Reprod 5: 168-174 [Abstract] [Full Text]
Horvath, G., Serru, V., Clay, D., Billard, M., Boucheix, C., Rubinstein, E. (1998). CD19 Is Linked to the Integrin-associated Tetraspans CD9, CD81, and CD82. J. Biol. Chem. 273: 30537-30543 [Abstract] [Full Text]
Huang, C.-l., Kohno, N., Ogawa, E., Adachi, M., Taki, T., Miyake, M. (1998). Correlation of Reduction in MRP-1/CD9 and KAI1/CD82 Expression with Recurrences in Breast Cancer Patients. Am. J. Pathol. 153: 973-983 [Abstract] [Full Text]
Yanez-Mo, M., Alfranca, A., Cabanas, C., Marazuela, M., Tejedor, R., Angeles Ursa, M., Ashman, L. K., de Landazuri, M. O., Sanchez-Madrid, F. (1998). Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with {alpha}3{beta}1 Integrin Localized at Endothelial Lateral Junctions. JCB 141: 791-804 [Abstract] [Full Text]
Geisert Jr., E. E., Yang, L., Irwin, M. H. (1996). Astrocyte Growth, Reactivity, and the Target of the Antiproliferative Antibody, TAPA. J. Neurosci. 16: 5478-5487 [Abstract] [Full Text]
Wu, X.-R., Medina, J. J., Sun, T.-T. (1995). Selective Interactions of UPIa and UPIb, Two Members of the Transmembrane 4 Superfamily, with Distinct Single Transmembrane-domained Proteins in Differentiated Urothelial Cells. J. Biol. Chem. 270: 29752-29759 [Abstract] [Full Text]
Shaw, A. R. E., Domanska, A., Mak, A., Gilchrist, A., Dobler, K., Visser, L., Poppema, S., Fliegel, L., Letarte, M., Willett, B. J. (1995). Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers beta1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation. J. Biol. Chem. 270: 24092-24099 [Abstract] [Full Text]
Dong, J., Lamb, P., Rinker-Schaeffer, C., Vukanovic, J, Ichikawa, T, Isaacs, J., Barrett, J. (1995). KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886 [Abstract]
Stipp, C. S., Orlicky, D., Hemler, M. E. (2001). FPRP, a Major, Highly Stoichiometric, Highly Specific CD81- and CD9-associated Protein. J. Biol. Chem. 276: 4853-4862 [Abstract] [Full Text]
Stipp, C. S., Kolesnikova, T. V., Hemler, M. E. (2001). EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily. J. Biol. Chem. 276: 40545-40554 [Abstract] [Full Text]
Nakamura, K., Mitamura, T., Takahashi, T., Kobayashi, T., Mekada, E. (2000). Importance of the Major Extracellular Domain of CD9 and the Epidermal Growth Factor (EGF)-like Domain of Heparin-binding EGF-like Growth Factor for Up-regulation of Binding and Activity. J. Biol. Chem. 275: 18284-18290 [Abstract] [Full Text]
Hakomori, S.-i. (2002). Inaugural Article: The glycosynapse. Proc. Natl. Acad. Sci. USA 99: 225-232 [Abstract] [Full Text]