Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression

doi:10.1084/jem.174.4.915

This Article

	Full Text (PDF, 961K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by de Waal Malefyt, R.
	Articles by de Vries, J. E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by de Waal Malefyt, R.
	Articles by de Vries, J. E.


Social Bookmarking

	What's this?

ARTICLES

Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression

R de Waal Malefyt, J Haanen, H Spits, MG Roncarolo, A te Velde, C Figdor, K Johnson, R Kastelein, H Yssel and JE de Vries
DNAX Research Institute, Human Immunology, Palo Alto, California 94304.

Interleukin 10 (IL-10) and viral IL-10 (v-IL-10) strongly reduced antigen-specific proliferation of human T cells and CD4+ T cell clones when monocytes were used as antigen-presenting cells. In contrast, IL- 10 and v-IL-10 did not affect the proliferative responses to antigens presented by autologous Epstein-Barr virus-lymphoblastoid cell line (EBV-LCL). Inhibition of antigen-specific T cell responses was associated with downregulation of constitutive, as well as interferon gamma- or IL-4-induced, class II MHC expression on monocytes by IL-10 and v-IL-10, resulting in the reduction in antigen-presenting capacity of these cells. In contrast, IL-10 and v-IL-10 had no effect on class II major histocompatibility complex (MHC) expression on EBV-LCL. The reduced antigen-presenting capacity of monocytes correlated with a decreased capacity to mobilize intracellular Ca2+ in the responder T cell clones. The diminished antigen-presenting capacities of monocytes were not due to inhibitory effects of IL-10 and v-IL-10 on antigen processing, since the proliferative T cell responses to antigenic peptides, which did not require processing, were equally well inhibited. Furthermore, the inhibitory effects of IL-10 and v-IL-10 on antigen-specific proliferative T cell responses could not be neutralized by exogenous IL-2 or IL-4. Although IL-10 and v-IL-10 suppressed IL-1 alpha, IL-1 beta, tumor necrosis factor alpha (TNF- alpha), and IL-6 production by monocytes, it was excluded that these cytokines played a role in antigen-specific T cell proliferation, since normal antigen-specific responses were observed in the presence of neutralizing anti-IL-1, -IL-6, and -TNF-alpha mAbs. Furthermore, addition of saturating concentrations of IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha to the cultures had no effect on the reduced proliferative T cell responses in the presence of IL-10, or v-IL-10. Collectively, our data indicate that IL-10 and v-IL-10 can completely prevent antigen-specific T cell proliferation by inhibition of the antigen-presenting capacity of monocytes through downregulation of class II MHC antigens on monocytes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Cheung, A. K. L., Gottlieb, D. J., Plachter, B., Pepperl-Klindworth, S., Avdic, S., Cunningham, A. L., Abendroth, A., Slobedman, B. (2009). The role of the human cytomegalovirus UL111A gene in down-regulating CD4+ T-cell recognition of latently infected cells: implications for virus elimination during latency. Blood 114: 4128-4137 [Abstract] [Full Text]
Slobedman, B., Barry, P. A., Spencer, J. V., Avdic, S., Abendroth, A. (2009). Virus-Encoded Homologs of Cellular Interleukin-10 and Their Control of Host Immune Function. J. Virol. 83: 9618-9629 [Full Text]
Hughes, S. M., Amadi, B., Mwiya, M., Nkamba, H., Tomkins, A., Goldblatt, D. (2009). Dendritic Cell Anergy Results from Endotoxemia in Severe Malnutrition. J. Immunol. 183: 2818-2826 [Abstract] [Full Text]
Gabrysova, L., Nicolson, K. S., Streeter, H. B., Verhagen, J., Sabatos-Peyton, C. A., Morgan, D. J., Wraith, D. C. (2009). Negative feedback control of the autoimmune response through antigen-induced differentiation of IL-10-secreting Th1 cells. JEM 206: 1755-1767 [Abstract] [Full Text]
Brockman, M. A., Kwon, D. S., Tighe, D. P., Pavlik, D. F., Rosato, P. C., Sela, J., Porichis, F., Le Gall, S., Waring, M. T., Moss, K., Jessen, H., Pereyra, F., Kavanagh, D. G., Walker, B. D., Kaufmann, D. E. (2009). IL-10 is up-regulated in multiple cell types during viremic HIV infection and reversibly inhibits virus-specific T cells. Blood 114: 346-356 [Abstract] [Full Text]
Sandanger, O., Ryan, L., Bohnhorst, J., Iversen, A.-C., Husebye, H., Halaas, O., Landro, L., Aukrust, P., Froland, S. S., Elson, G., Visintin, A., Oktedalen, O., Damas, J. K., Sundan, A., Golenbock, D., Espevik, T. (2009). IL-10 Enhances MD-2 and CD14 Expression in Monocytes and the Proteins Are Increased and Correlated in HIV-Infected Patients. J. Immunol. 182: 588-595 [Abstract] [Full Text]
Chakrabarti, A., Sadler, A. J., Kar, N., Young, H. A., Silverman, R. H., Williams, B. R. G. (2008). Protein Kinase R-dependent Regulation of Interleukin-10 in Response to Double-stranded RNA. J. Biol. Chem. 283: 25132-25139 [Abstract] [Full Text]
Stumpf, C., Seybold, K., Petzi, S., Wasmeier, G., Raaz, D., Yilmaz, A., Anger, T., Daniel, W. G., Garlichs, C. D. (2008). Interleukin-10 improves left ventricular function in rats with heart failure subsequent to myocardial infarction. Eur J Heart Fail 10: 733-739 [Abstract] [Full Text]
Santegoets, S. J. A. M., Gibbs, S., Kroeze, K., van de Ven, R., Scheper, R. J., Borrebaeck, C. A., de Gruijl, T. D., Lindstedt, M. (2008). Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. J. Leukoc. Biol. 84: 143-151 [Abstract] [Full Text]
Goverdhan, S V, Ennis, S, Hannan, S R, Madhusudhana, K C, Cree, A J, Luff, A J, Lotery, A J (2008). Interleukin-8 promoter polymorphism -251A/T is a risk factor for age-related macular degeneration. Br J Ophthalmol 92: 537-540 [Abstract] [Full Text]
Stowell, S. R., Qian, Y., Karmakar, S., Koyama, N. S., Dias-Baruffi, M., Leffler, H., McEver, R. P., Cummings, R. D. (2008). Differential Roles of Galectin-1 and Galectin-3 in Regulating Leukocyte Viability and Cytokine Secretion. J. Immunol. 180: 3091-3102 [Abstract] [Full Text]
Deniz, G., Erten, G., Kucuksezer, U. C., Kocacik, D., Karagiannidis, C., Aktas, E., Akdis, C. A., Akdis, M. (2008). Regulatory NK Cells Suppress Antigen-Specific T Cell Responses. J. Immunol. 180: 850-857 [Abstract] [Full Text]
Barrionuevo, P., Cassataro, J., Delpino, M. V., Zwerdling, A., Pasquevich, K. A., Samartino, C. G., Wallach, J. C., Fossati, C. A., Giambartolomei, G. H. (2008). Brucella abortus Inhibits Major Histocompatibility Complex Class II Expression and Antigen Processing through Interleukin-6 Secretion via Toll-Like Receptor 2. Infect. Immun. 76: 250-262 [Abstract] [Full Text]
Lee, J.-B., Lee, K.-A, Chang, J. (2007). Phenotypic changes induced by IL-12 priming regulate effector and memory CD8 T cell differentiation. Int Immunol 0: dxm072v1-dxm072 [Abstract] [Full Text]
Gray, J., Mao, J. T., Szabo, E., Kelley, M., Kurie, J., Bepler, G. (2007). Lung Cancer Chemoprevention: ACCP Evidence-Based Clinical Practice Guidelines (2nd Edition). Chest 132: 56S-68S [Abstract] [Full Text]
Andoh, M., Zhang, G., Russell-Lodrigue, K. E., Shive, H. R., Weeks, B. R., Samuel, J. E. (2007). T Cells Are Essential for Bacterial Clearance, and Gamma Interferon, Tumor Necrosis Factor Alpha, and B Cells Are Crucial for Disease Development in Coxiella burnetii Infection in Mice. Infect. Immun. 75: 3245-3255 [Abstract] [Full Text]
Divangahi, M., Demoule, A., Danialou, G., Yahiaoui, L., Bao, W., Xing, Z., Petrof, B. J. (2007). Impact of IL-10 on Diaphragmatic Cytokine Expression and Contractility during Pseudomonas Infection. Am. J. Respir. Cell Mol. Bio. 36: 504-512 [Abstract] [Full Text]
Pilette, C., Nouri-Aria, K. T., Jacobson, M. R., Wilcock, L. K., Detry, B., Walker, S. M., Francis, J. N., Durham, S. R. (2007). Grass Pollen Immunotherapy Induces an Allergen-Specific IgA2 Antibody Response Associated with Mucosal TGF-beta Expression. J. Immunol. 178: 4658-4666 [Abstract] [Full Text]
Gee, K., Angel, J. B., Mishra, S., Blahoianu, M. A., Kumar, A. (2007). IL-10 Regulation by HIV-Tat in Primary Human Monocytic Cells: Involvement of Calmodulin/Calmodulin-Dependent Protein Kinase-Activated p38 MAPK and Sp-1 and CREB-1 Transcription Factors. J. Immunol. 178: 798-807 [Abstract] [Full Text]
Yoon, S. I., Logsdon, N. J., Sheikh, F., Donnelly, R. P., Walter, M. R. (2006). Conformational Changes Mediate Interleukin-10 Receptor 2 (IL-10R2) Binding to IL-10 and Assembly of the Signaling Complex. J. Biol. Chem. 281: 35088-35096 [Abstract] [Full Text]
Foulds, K. E., Rotte, M. J., Seder, R. A. (2006). IL-10 Is Required for Optimal CD8 T Cell Memory following Listeria monocytogenes Infection. J. Immunol. 177: 2565-2574 [Abstract] [Full Text]
Ismail, N., Stevenson, H. L., Walker, D. H. (2006). Role of Tumor Necrosis Factor Alpha (TNF-{alpha}) and Interleukin-10 in the Pathogenesis of Severe Murine Monocytotropic Ehrlichiosis: Increased Resistance of TNF Receptor p55- and p75-Deficient Mice to Fatal Ehrlichial Infection. Infect. Immun. 74: 1846-1856 [Abstract] [Full Text]
Kim, E., Kliger, Y. (2005). Discovering hidden viral piracy. Bioinformatics 21: 4216-4222 [Abstract] [Full Text]
Mu, W., Ouyang, X., Agarwal, A., Zhang, L., Long, D. A., Cruz, P. E., Roncal, C. A., Glushakova, O. Y., Chiodo, V. A., Atkinson, M. A., Hauswirth, W. W., Flotte, T. R., Rodriguez-Iturbe, B., Johnson, R. J. (2005). IL-10 Suppresses Chemokines, Inflammation, and Fibrosis in a Model of Chronic Renal Disease. J. Am. Soc. Nephrol. 16: 3651-3660 [Abstract] [Full Text]
Atan, D., Turner, S. J., Kilmartin, D. J., Forrester, J. V., Bidwell, J., Dick, A. D., Churchill, A. J. (2005). Cytokine Gene Polymorphism in Sympathetic Ophthalmia. IOVS 46: 4245-4250 [Abstract] [Full Text]
Sendide, K., Deghmane, A.-E., Pechkovsky, D., Av-Gay, Y., Talal, A., Hmama, Z. (2005). Mycobacterium bovis BCG Attenuates Surface Expression of Mature Class II Molecules through IL-10-Dependent Inhibition of Cathepsin S. J. Immunol. 175: 5324-5332 [Abstract] [Full Text]
Cooper, A., Robinson, S. J., Pickard, C., Jackson, C. L., Friedmann, P. S., Healy, E. (2005). {alpha}-Melanocyte-Stimulating Hormone Suppresses Antigen-Induced Lymphocyte Proliferation in Humans Independently of Melanocortin 1 Receptor Gene Status. J. Immunol. 175: 4806-4813 [Abstract] [Full Text]
Safadi, R., Alvarez, C. E., Ohta, M., Brimnes, J., Kraus, T., Mehal, W., Bromberg, J., Mayer, L., Friedman, S. L. (2005). Enhanced Oral Tolerance in Transgenic Mice with Hepatocyte Secretion of IL-10. J. Immunol. 175: 3577-3583 [Abstract] [Full Text]
Bouman, A., Heineman, M. J., Faas, M. M. (2005). Sex hormones and the immune response in humans. Hum Reprod Update 11: 411-423 [Abstract] [Full Text]
Rahimi, A. A. R., Gee, K., Mishra, S., Lim, W., Kumar, A. (2005). STAT-1 Mediates the Stimulatory Effect of IL-10 on CD14 Expression in Human Monocytic Cells. J. Immunol. 174: 7823-7832 [Abstract] [Full Text]
Nakagome, K., Dohi, M., Okunishi, K., Komagata, Y., Nagatani, K., Tanaka, R., Miyazaki, J.-i., Yamamoto, K. (2005). In Vivo IL-10 Gene Delivery Suppresses Airway Eosinophilia and Hyperreactivity by Down-Regulating APC Functions and Migration without Impairing the Antigen-Specific Systemic Immune Response in a Mouse Model of Allergic Airway Inflammation. J. Immunol. 174: 6955-6966 [Abstract] [Full Text]
Furukawa, H., Oshima, K., Tung, T., Cui, G., Laks, H., Sen, L. (2005). Liposome-Mediated Combinatorial Cytokine Gene Therapy Induces Localized Synergistic Immunosuppression and Promotes Long-Term Survival of Cardiac Allografts. J. Immunol. 174: 6983-6992 [Abstract] [Full Text]
Chen, S., Kapturczak, M. H., Wasserfall, C., Glushakova, O. Y., Campbell-Thompson, M., Deshane, J. S., Joseph, R., Cruz, P. E., Hauswirth, W. W., Madsen, K. M., Croker, B. P., Berns, K. I., Atkinson, M. A., Flotte, T. R., Tisher, C. C., Agarwal, A. (2005). Interleukin 10 attenuates neointimal proliferation and inflammation in aortic allografts by a heme oxygenase-dependent pathway. Proc. Natl. Acad. Sci. USA 102: 7251-7256 [Abstract] [Full Text]
Kang, S. S., Allen, P. M. (2005). Priming in the Presence of IL-10 Results in Direct Enhancement of CD8+ T Cell Primary Responses and Inhibition of Secondary Responses. J. Immunol. 174: 5382-5389 [Abstract] [Full Text]
Brustoski, K., Moller, U., Kramer, M., Petelski, A., Brenner, S., Palmer, D. R., Bongartz, M., Kremsner, P. G., Luty, A. J. F., Krzych, U. (2005). IFN-{gamma} and IL-10 Mediate Parasite-Specific Immune Responses of Cord Blood Cells Induced by Pregnancy-Associated Plasmodium falciparum Malaria. J. Immunol. 174: 1738-1745 [Abstract] [Full Text]
Salvati, V M, Mazzarella, G, Gianfrani, C, Levings, M K, Stefanile, R, De Giulio, B, Iaquinto, G, Giardullo, N, Auricchio, S, Roncarolo, M G, Troncone, R (2005). Recombinant human interleukin 10 suppresses gliadin dependent T cell activation in ex vivo cultured coeliac intestinal mucosa. Gut 54: 46-53 [Abstract] [Full Text]
Grutz, G. (2005). New insights into the molecular mechanism of interleukin-10-mediated immunosuppression. J. Leukoc. Biol. 77: 3-15 [Abstract] [Full Text]
Connor, T. J., Harkin, A., Kelly, J. P. (2005). Methylenedioxymethamphetamine Suppresses Production of the Proinflammatory Cytokine Tumor Necrosis Factor-{alpha} Independent of a {beta}-Adrenoceptor-Mediated Increase in Interleukin-10. J. Pharmacol. Exp. Ther. 312: 134-143 [Abstract] [Full Text]
Lyke, K. E., Burges, R., Cissoko, Y., Sangare, L., Dao, M., Diarra, I., Kone, A., Harley, R., Plowe, C. V., Doumbo, O. K., Sztein, M. B. (2004). Serum Levels of the Proinflammatory Cytokines Interleukin-1 Beta (IL-1{beta}), IL-6, IL-8, IL-10, Tumor Necrosis Factor Alpha, and IL-12(p70) in Malian Children with Severe Plasmodium falciparum Malaria and Matched Uncomplicated Malaria or Healthy Controls. Infect. Immun. 72: 5630-5637 [Abstract] [Full Text]
Steinke, J. W., Barekzi, E., Hagman, J., Borish, L. (2004). Functional Analysis of -571 IL-10 Promoter Polymorphism Reveals a Repressor Element Controlled by Sp1. J. Immunol. 173: 3215-3222 [Abstract] [Full Text]
Donnelly, R. P., Sheikh, F., Kotenko, S. V., Dickensheets, H. (2004). The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J. Leukoc. Biol. 76: 314-321 [Abstract] [Full Text]
Kurte, M., Lopez, M., Aguirre, A., Escobar, A., Aguillon, J. C., Charo, J., Larsen, C. G., Kiessling, R., Salazar-Onfray, F. (2004). A Synthetic Peptide Homologous to Functional Domain of Human IL-10 Down-Regulates Expression of MHC Class I and Transporter Associated with Antigen Processing 1/2 in Human Melanoma Cells. J. Immunol. 173: 1731-1737 [Abstract] [Full Text]
Wohlfert, E. A., Callahan, M. K., Clark, R. B. (2004). Resistance to CD4+CD25+ Regulatory T Cells and TGF-{beta} in Cbl-b-/- Mice. J. Immunol. 173: 1059-1065 [Abstract] [Full Text]
Carbonneil, C., Saidi, H., Donkova-Petrini, V., Weiss, L. (2004). Dendritic cells generated in the presence of interferon-{alpha} stimulate allogeneic CD4+ T-cell proliferation: modulation by autocrine IL-10, enhanced T-cell apoptosis and T regulatory type 1 cells. Int Immunol 16: 1037-1052 [Abstract] [Full Text]
Avery, P. R., Hoover, E. A. (2004). Gamma Interferon/Interleukin 10 Balance in Tissue Lymphocytes Correlates with Down Modulation of Mucosal Feline Immunodeficiency Virus Infection. J. Virol. 78: 4011-4019 [Abstract] [Full Text]
Verreck, F. A. W., de Boer, T., Langenberg, D. M. L., Hoeve, M. A., Kramer, M., Vaisberg, E., Kastelein, R., Kolk, A., de Waal-Malefyt, R., Ottenhoff, T. H. M. (2004). Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria. Proc. Natl. Acad. Sci. USA 101: 4560-4565 [Abstract] [Full Text]
Shumilla, J. A., Lacaille, V., Hornell, T. M. C., Huang, J., Narasimhan, S., Relman, D. A., Mellins, E. D. (2004). Bordetella pertussis Infection of Primary Human Monocytes Alters HLA-DR Expression. Infect. Immun. 72: 1450-1462 [Abstract] [Full Text]
Hesse, M., Piccirillo, C. A., Belkaid, Y., Prufer, J., Mentink-Kane, M., Leusink, M., Cheever, A. W., Shevach, E. M., Wynn, T. A. (2004). The Pathogenesis of Schistosomiasis Is Controlled by Cooperating IL-10-Producing Innate Effector and Regulatory T Cells. J. Immunol. 172: 3157-3166 [Abstract] [Full Text]
Mao, J. T., Roth, M. D., Serio, K. J., Baratelli, F., Zhu, L., Holmes, E. C., Strieter, R. M., Dubinett, S. M. (2003). Celecoxib Modulates the Capacity for Prostaglandin E2 and Interleukin-10 Production in Alveolar Macrophages from Active Smokers. Clin. Cancer Res. 9: 5835-5841 [Abstract] [Full Text]
Fontana, S., Moratto, D., Mangal, S., De Francesco, M., Vermi, W., Ferrari, S., Facchetti, F., Kutukculer, N., Fiorini, C., Duse, M., Das, P. K., Notarangelo, L. D., Plebani, A., Badolato, R. (2003). Functional defects of dendritic cells in patients with CD40 deficiency. Blood 102: 4099-4106 [Abstract] [Full Text]
Lohning, M., Richter, A., Stamm, T., Hu-Li, J., Assenmacher, M., Paul, W. E., Radbruch, A. (2003). Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. USA 100: 12307-12312 [Abstract] [Full Text]
Tomescu, C., Law, W. K., Kedes, D. H. (2003). Surface Downregulation of Major Histocompatibility Complex Class I, PE-CAM, and ICAM-1 following De Novo Infection of Endothelial Cells with Kaposi's Sarcoma-Associated Herpesvirus. J. Virol. 77: 9669-9684 [Abstract] [Full Text]
Asadullah, K., Sterry, W., Volk, H. D. (2003). Interleukin-10 Therapy--Review of a New Approach. Pharmacol. Rev. 55: 241-269 [Abstract] [Full Text]
Hauber, H-P, Gholami, D, Meyer, A, Pforte, A (2003). Increased interleukin-13 expression in patients with sarcoidosis. Thorax 58: 519-524 [Abstract] [Full Text]
Schroder, M., Meisel, C., Buhl, K., Profanter, N., Sievert, N., Volk, H.-D., Grutz, G. (2003). Different Modes of IL-10 and TGF-{beta} to Inhibit Cytokine-Dependent IFN-{gamma} Production: Consequences for Reversal of Lipopolysaccharide Desensitization. J. Immunol. 170: 5260-5267 [Abstract] [Full Text]
Schierack, P., Lucius, R., Sonnenburg, B., Schilling, K., Hartmann, S. (2003). Parasite-Specific Immunomodulatory Functions of Filarial Cystatin. Infect. Immun. 71: 2422-2429 [Abstract] [Full Text]
Mahot, S., Sergeant, A., Drouet, E., Gruffat, H. (2003). A novel function for the Epstein-Barr virus transcription factor EB1/Zta: induction of transcription of the hIL-10 gene. J. Gen. Virol. 84: 965-974 [Abstract] [Full Text]
Zhu, L. X., Sharma, S., Gardner, B., Escuadro, B., Atianzar, K., Tashkin, D. P., Dubinett, S. M. (2003). IL-10 Mediates Sigma1 Receptor-Dependent Suppression of Antitumor Immunity. J. Immunol. 170: 3585-3591 [Abstract] [Full Text]
Schuerwegh, A. J., Van Offel, J. F., Stevens, W. J., Bridts, C. H., De Clerck, L. S. (2003). Influence of therapy with chimeric monoclonal tumour necrosis factor-{alpha} antibodies on intracellular cytokine profiles of T lymphocytes and monocytes in rheumatoid arthritis patients. Rheumatology (Oxford) 42: 541-548 [Abstract] [Full Text]
Von der Thusen, J. H., Kuiper, J., Van Berkel, T. J. C., Biessen, E. A. L. (2003). Interleukins in Atherosclerosis: Molecular Pathways and Therapeutic Potential. Pharmacol. Rev. 55: 133-166 [Abstract] [Full Text]
Yang, J., Reutzel-Selke, A., Steier, C., Jurisch, A., Tullius, S. G., Sawitzki, B., Kolls, J., Volk, H.-D., Ritter, T. (2003). Targeting of Macrophage Activity by Adenovirus-Mediated Intragraft Overexpression of TNFRp55-Ig, IL-12p40, and vIL-10 Ameliorates Adenovirus-Mediated Chronic Graft Injury, whereas Stimulation of Macrophages by Overexpression of IFN-{gamma} Accelerates Chronic Graft Injury in a Rat Renal Allograft Model. J. Am. Soc. Nephrol. 14: 214-225 [Abstract] [Full Text]
Karlsson, H., Hessle, C., Rudin, A. (2002). Innate Immune Responses of Human Neonatal Cells to Bacteria from the Normal Gastrointestinal Flora. Infect. Immun. 70: 6688-6696 [Abstract] [Full Text]
Wille, U., Villegas, E. N., Craig, L., Peach, R., Hunter, C. A. (2002). Contribution of Interleukin-12 (IL-12) and the CD28/B7 and CD40/CD40 Ligand Pathways to the Development of a Pathological T-Cell Response in IL-10-Deficient Mice. Infect. Immun. 70: 6940-6947 [Abstract] [Full Text]
Turner, J., Gonzalez-Juarrero, M., Ellis, D. L., Basaraba, R. J., Kipnis, A., Orme, I. M., Cooper, A. M. (2002). In Vivo IL-10 Production Reactivates Chronic Pulmonary Tuberculosis in C57BL/6 Mice. J. Immunol. 169: 6343-6351 [Abstract] [Full Text]
Lazetic, S., Leong, S. R., Chang, J. C-C., Ong, R., Dawes, G., Punnonen, J. (2002). Chimeric Co-stimulatory Molecules That Selectively Act through CD28 or CTLA-4 on Human T Cells. J. Biol. Chem. 277: 38660-38668 [Abstract] [Full Text]
Miura, Y., Thoburn, C. J., Bright, E. C., Chen, W., Nakao, S., Hess, A. D. (2002). Cytokine and chemokine profiles in autologous graft-versus-host disease (GVHD): interleukin 10 and interferon gamma may be critical mediators for the development of autologous GVHD. Blood 100: 2650-2658 [Abstract] [Full Text]
Daddi, N., Suda, T., D'Ovidio, F., Kanaan, S. A., Tagawa, T., Grapperhaus, K., Kozower, B. D., Ritter, J. H., Yew, N. S, Mohanakumar, T., Patterson, G. A. (2002). Recipient intramuscular cotransfection of naked plasmid transforming growth factor {beta}1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg. 124: 259-269 [Abstract] [Full Text]
Mendez-Samperio, P., Garcia, E., Vazquez, A., Palma, J. (2002). Regulation of Interleukin-8 by Interleukin-10 and Transforming Growth Factor {beta} in Human Monocytes Infected with Mycobacterium bovis. CVI 9: 802-807 [Abstract] [Full Text]
Yang, Z., Chen, M., Wu, R., Fialkow, L. B., Bromberg, J. S., McDuffie, M., Naji, A., Nadler, J. L. (2002). Suppression of Autoimmune Diabetes by Viral IL-10 Gene Transfer. J. Immunol. 168: 6479-6485 [Abstract] [Full Text]
Mathurin, P., Xiong, S., Kharbanda, K. K., Veal, N., Miyahara, T., Motomura, K., Rippe, R. A., Bachem, M. G., Tsukamoto, H. (2002). IL-10 receptor and coreceptor expression in quiescent and activated hepatic stellate cells. Am. J. Physiol. Gastrointest. Liver Physiol. 282: G981-G990 [Abstract] [Full Text]
Erb, K. J., Trujillo, C., Fugate, M., Moll, H. (2002). Infection with the Helminth Nippostrongylus brasiliensis Does Not Interfere with Efficient Elimination of Mycobacterium bovis BCG from the Lungs of Mice. CVI 9: 727-730 [Abstract] [Full Text]
Giambartolomei, G. H., Dennis, V. A., Lasater, B. L., Murthy, P. K., Philipp, M. T. (2002). Autocrine and Exocrine Regulation of Interleukin-10 Production in THP-1 Cells Stimulated with Borrelia burgdorferi Lipoproteins. Infect. Immun. 70: 1881-1888 [Abstract] [Full Text]
Jones, D. E., Ackermann, M. R., Wille, U., Hunter, C. A., Scott, P. (2002). Early Enhanced Th1 Response after Leishmania amazonensis Infection of C57BL/6 Interleukin-10-Deficient Mice Does Not Lead to Resolution of Infection. Infect. Immun. 70: 2151-2158 [Abstract] [Full Text]
Tezuka, H., Imai, S., Muto, R., Furuhashi, Y., Fujita, K. (2002). Recombinant Dirofilaria immitis Polyprotein That Stimulates Murine B Cells To Produce Nonspecific Polyclonal Immunoglobulin E Antibody. Infect. Immun. 70: 1235-1244 [Abstract] [Full Text]
Harizi, H., Juzan, M., Pitard, V., Moreau, J.-F., Gualde, N. (2002). Cyclooxygenase-2-Issued Prostaglandin E2 Enhances the Production of Endogenous IL-10, Which Down-Regulates Dendritic Cell Functions. J. Immunol. 168: 2255-2263 [Abstract] [Full Text]
Soltys, J., Bonfield, T., Chmiel, J., Berger, M. (2002). Functional IL-10 Deficiency in the Lung of Cystic Fibrosis (cftr-/-) and IL-10 Knockout Mice Causes Increased Expression and Function of B7 Costimulatory Molecules on Alveolar Macrophages. J. Immunol. 168: 1903-1910 [Abstract] [Full Text]
Spencer, J. V., Lockridge, K. M., Barry, P. A., Lin, G., Tsang, M., Penfold, M. E. T., Schall, T. J. (2002). Potent Immunosuppressive Activities of Cytomegalovirus- Encoded Interleukin-10. J. Virol. 76: 1285-1292 [Abstract] [Full Text]
Tilg, H, van Montfrans, C, van den Ende, A, Kaser, A, van Deventer, S J H, Schreiber, S, Gregor, M, Ludwiczek, O, Rutgeerts, P, Gasche, C, Koningsberger, J C, Abreu, L, Kuhn, I, Cohard, M, LeBeaut, A, Grint, P, Weiss, G (2002). Treatment of Crohn's disease with recombinant human interleukin 10 induces the proinflammatory cytokine interferon {gamma}. Gut 50: 191-195 [Abstract] [Full Text]
Ibe, S., Qin, Z., Schuler, T., Preiss, S., Blankenstein, T. (2001). Tumor Rejection by Disturbing Tumor Stroma Cell Interactions. JEM 194: 1549-1560 [Abstract] [Full Text]
Vancheri, C., Mastruzzo, C., Tomaselli, V., Sortino, M. A., D'Amico, L., Bellistri, G., Pistorio, M. P., Salinaro, E. T., Palermo, F., Mistretta, A., Crimi, N. (2001). Normal Human Lung Fibroblasts Differently Modulate Interleukin-10 and Interleukin-12 Production by Monocytes . Implications for an Altered Immune Response in Pulmonary Chronic Inflammation. Am. J. Respir. Cell Mol. Bio. 25: 592-599 [Abstract] [Full Text]
McInnes, I. B., Illei, G. G., Danning, C. L., Yarboro, C. H., Crane, M., Kuroiwa, T., Schlimgen, R., Lee, E., Foster, B., Flemming, D., Prussin, C., Fleisher, T. A., Boumpas, D. T. (2001). IL-10 Improves Skin Disease and Modulates Endothelial Activation and Leukocyte Effector Function in Patients with Psoriatic Arthritis. J. Immunol. 167: 4075-4082 [Abstract] [Full Text]
Wakkach, A., Cottrez, F., Groux, H. (2001). Differentiation of Regulatory T Cells 1 Is Induced by CD2 Costimulation. J. Immunol. 167: 3107-3113 [Abstract] [Full Text]
Schonemeyer, A., Lucius, R., Sonnenburg, B., Brattig, N., Sabat, R., Schilling, K., Bradley, J., Hartmann, S. (2001). Modulation of Human T Cell Responses and Macrophage Functions by Onchocystatin, a Secreted Protein of the Filarial Nematode Onchocerca volvulus. J. Immunol. 167: 3207-3215 [Abstract] [Full Text]
Muller-Steinhardt, M., Kock, N., Hartel, C., Kirchner, H., Steinhoff, J. (2001). Production of monokines in patients under polysulphone haemodiafiltration is influenced by the ultrafiltration flow rate. Nephrol Dial Transplant 16: 1830-1837 [Abstract] [Full Text]
Silva, R. A., Pais, T. F., Appelberg, R. (2001). Blocking the Receptor for IL-10 Improves Antimycobacterial Chemotherapy and Vaccination. J. Immunol. 167: 1535-1541 [Abstract] [Full Text]
Itano, H., Mora, B. N., Zhang, W., Ritter, J. H., McCarthy, T. J., Yew, N. S., Mohanakumar, T., Patterson, G. A. (2001). Lipid-mediated ex vivo gene transfer of viral interleukin 10 in rat lung allotransplantation. J. Thorac. Cardiovasc. Surg. 122: 29-38 [Abstract] [Full Text]
Takayama, T., Morelli, A. E., Onai, N., Hirao, M., Matsushima, K., Tahara, H., Thomson, A. W. (2001). Mammalian and Viral IL-10 Enhance C-C Chemokine Receptor 5 but Down-Regulate C-C Chemokine Receptor 7 Expression by Myeloid Dendritic Cells: Impact on Chemotactic Responses and In Vivo Homing Ability. J. Immunol. 166: 7136-7143 [Abstract] [Full Text]
Fadok, V. A., Bratton, D. L., Guthrie, L., Henson, P. M. (2001). Differential Effects of Apoptotic Versus Lysed Cells on Macrophage Production of Cytokines: Role of Proteases. J. Immunol. 166: 6847-6854 [Abstract] [Full Text]
Gerber, J. S., Mosser, D. M. (2001). Reversing Lipopolysaccharide Toxicity by Ligating the Macrophage Fc{{gamma}} Receptors. J. Immunol. 166: 6861-6868 [Abstract] [Full Text]
Quattrocchi, E., Dallman, M. J., Dhillon, A. P., Quaglia, A., Bagnato, G., Feldmann, M. (2001). Murine IL-10 Gene Transfer Inhibits Established Collagen-Induced Arthritis and Reduces Adenovirus-Mediated Inflammatory Responses in Mouse Liver. J. Immunol. 166: 5970-5978 [Abstract] [Full Text]
Knight, D. A., Holt, P. G., Sly, P. D. (2001). Lipopolysaccharide Inhibits the Late-Phase Response to Allergen by Altering Nitric Oxide Synthase Activity and Interleukin-10. Am. J. Respir. Cell Mol. Bio. 24: 640-646 [Abstract] [Full Text]
Kawamoto, S., Nitta, Y., Tashiro, F., Nakano, A., Yamato, E., Tahara, H., Tabayashi, K., Miyazaki, J.-i. (2001). Suppression of Th1 cell activation and prevention of autoimmune diabetes in NOD mice by local expression of viral IL-10. Int Immunol 13: 685-694 [Abstract] [Full Text]
Kawasaki, T., Ogata, M., Kawasaki, C., Tomihisa, T., Okamoto, K., Shigematsu, A. (2001). Surgical Stress Induces Endotoxin Hyporesponsiveness and an Early Decrease of Monocyte mCD14 and HLA-DR Expression During Surgery. Anesth. Analg. 92: 1322-1326 [Abstract] [Full Text]
Crawley, E., Kon, S., Woo, P. (2001). Hereditary predisposition to low interleukin-10 production in children with extended oligoarticular juvenile idiopathic arthritis. Rheumatology (Oxford) 40: 574-578 [Abstract] [Full Text]
Itano, H., Zhang, W., Ritter, J. H., McCarthy, T. J., Yew, N. S., Mohanakumar, T., Patterson, G. A. (2001). Endobronchial transfection of naked viral interleukin-10 gene in rat lung allotransplantation. Ann. Thorac. Surg. 71: 1126-1133 [Abstract] [Full Text]
Qin, L., Ding, Y., Tahara, H., Bromberg, J. S. (2001). Viral IL-10-Induced Immunosuppression Requires Th2 Cytokines and Impairs APC Function Within the Allograft. J. Immunol. 166: 2385-2393 [Abstract] [Full Text]
Wigginton, J. E., Kirschner, D. (2001). A Model to Predict Cell-Mediated Immune Regulatory Mechanisms During Human Infection with Mycobacterium tuberculosis. J. Immunol. 166: 1951-1967 [Abstract] [Full Text]
Boussiotis, V. A., Chen, Z.-M., Zeller, J. C., Murphy, W. J., Berezovskaya, A., Narula, S., Roncarolo, M. G., Blazar, B. R. (2001). Altered T-cell receptor + CD28-mediated signaling and blocked cell cycle progression in interleukin 10 and transforming growth factor-{beta}-treated alloreactive T cells that do not induce graft-versus-host disease. Blood 97: 565-571 [Abstract] [Full Text]