T cell receptor gamma and delta gene junctional sequences in SCID mice: excessive P nucleotide insertion

doi:10.1084/jem.174.4.769

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T cell receptor gamma and delta gene junctional sequences in SCID mice: excessive P nucleotide insertion

LJ Kienker, WA Kuziel and PW Tucker
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

The severe combined immunodeficiency (SCID) mutation has been postulated to affect a V(D)J recombinase activity involved in coding joint formation. Analysis of 38 joints from 34 distinct sequences of normally rearranged T cell receptor (TCR) gamma and delta genes from adult, SCID thymocytes reveals coding joints with an increased number of P nucleotides. One-third of P sequences are greater than or equal to 4 nucleotides in length and P elements of up to 15 bases are observed. This suggests that the SCID defect deregulates P nucleotide addition. Consequently, essential V(D)J recombination intermediates may seldom be generated.
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