Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8

doi:10.1084/jem.173.3.771

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Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interleukin 8

F Bazzoni, MA Cassatella, F Rossi, M Ceska, B Dewald and M Baggiolini
Institute of General Pathology, University of Verona, Italy.

After phagocytosis of yeast opsonized with IgG, neutrophil leukocytes (polymorphonuclear leukocytes [PMN]) expressed high levels of neutrophil-activating peptide 1/interleukin 8 (NAP-1/IL-8) mRNA, which peaked after 3-5 h and were still elevated after 18 h. A similar but quantitatively less prominent effect was obtained with lipopolysaccharide (LPS). After phagocytosis, but not after exposure to LPS, the PMN progressively released considerable amounts of NAP-1/IL-8 into the culture medium (18.6-50 ng/ml in 18 h). The peptide released was biologically active, as indicated by the transient elevation of cytosolic-free calcium in PMN exposed to aliquots of the culture supernatants, and desensitization by prestimulation of the cells with recombinant NAP-1/IL-8. By producing NAP-1/IL-8 at sites where they phagocytose invading microorganisms, PMN could enhance the recruitment of new defense cells.
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