Journal of Experimental Medicine, Vol 173, 731-741, Copyright © 1991 by Rockefeller University Press

ARTICLES

Characterization of somatically mutated S107 VH11-encoded anti-DNA autoantibodies derived from autoimmune (NZB x NZW)F1 mice

SM Behar, DL Lustgarten, S Corbet and MD Scharff
Department of Cell Biology, Irvington House Institute, Albert Einstein College of Medicine, Bronx, New York 10461.

We have studied 19 S107 heavy chain variable region gene (VH11)-encoded monoclonal antibodies from NZBWF1 mice. These studies show that a single VH gene can encode both antibodies to foreign antigens (anti- phosphorylcholine) and to self antigens (anti-double-stranded DNA) in the same animal. All of the anti-DNA antibodies contain many somatic mutations compared with the relevant germline genes. Since the anti-DNA antibodies were extensively somatically mutated and had undergone isotype switching, the response seems to be T cell dependent. While some of the antibodies appear to be the products of an antigen-driven and antigen-selected response, a number of characteristics of the antibodies suggest that forces other than antigen are contributing to the stimulation and selection of this response.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Linterman, M. A., Rigby, R. J., Wong, Raphael. K., Yu, D., Brink, R., Cannons, J. L., Schwartzberg, P. L., Cook, M. C., Walters, G. D., Vinuesa, C. G. (2009). Follicular helper T cells are required for systemic autoimmunity. JEM 206: 561-576 [Abstract] [Full Text]  
• Guth, A. M., Zhang, X., Smith, D., Detanico, T., Wysocki, L. J. (2003). Chromatin Specificity of Anti-Double-Stranded DNA Antibodies and a Role for Arg Residues in the Third Complementarity-Determining Region of the Heavy Chain. J. Immunol. 171: 6260-6266 [Abstract] [Full Text]  
• Pewzner-Jung, Y., Friedmann, D., Sonoda, E., Jung, S., Rajewsky, K., Eilat, D. (1998). B Cell Deletion, Anergy, and Receptor Editing in ""Knock In"" Mice Targeted with a Germline-Encoded or Somatically Mutated Anti-DNA Heavy Chain. J. Immunol. 161: 4634-4645 [Abstract] [Full Text]  
• Scharff, M. D., Poltoratsky, V., Green, N. S. (1997). The Promotion of V Region Hypermutation. JEM 185: 185-188 [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS