Heavy-chain class switch does not terminate somatic mutation

doi:10.1084/jem.172.2.531

This Article

	Full Text (PDF, 630K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Shan, H.
	Articles by Weigert, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Shan, H.
	Articles by Weigert, M.


Social Bookmarking

	What's this?

ARTICLES

Heavy-chain class switch does not terminate somatic mutation

H Shan, M Shlomchik and M Weigert
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.

We studied the H-chain class switch rearrangement in four groups of clonally related B cell hybridomas, to test the hypothesis that class switch terminates somatic mutation in a B cell. Using switch region- specific probes in Southern blot analysis individual mu-gamma switch rearrangement events can be distinguished. We show that clonally related IgG-producing hybridomas that differ by mutations often share a common switch rearrangement. This indicates that class switch in these cells did not terminate somatic mutation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Seifert, M., Kuppers, R. (2009). Molecular footprints of a germinal center derivation of human IgM+(IgD+)CD27+ B cells and the dynamics of memory B cell generation. JEM 206: 2659-2669 [Abstract] [Full Text]
Longacre, A., Sun, T., Goldsby, R. E., Preston, B. D., Storb, U. (2003). Ig gene somatic hypermutation in mice defective for DNA polymerase {delta} proofreading. Int Immunol 15: 477-481 [Abstract] [Full Text]
Zan, H., Cerutti, A., Dramitinos, P., Schaffer, A., Li, Z., Casali, P. (1999). Induction of Ig Somatic Hypermutation and Class Switching in a Human Monoclonal IgM+ IgD+ B Cell Line In Vitro: Definition of the Requirements and Modalities of Hypermutation. J. Immunol. 162: 3437-3447 [Abstract] [Full Text]
Aarts, W.M., Willemze, R., Bende, R.J., Meijer, C.J.L.M., Pals, S.T., van Noesel, C.J.M. (1998). VH Gene Analysis of Primary Cutaneous B-Cell Lymphomas: Evidence for Ongoing Somatic Hypermutation and Isotype Switching. Blood 92: 3857-3864 [Abstract] [Full Text]
Kaisho, T., Schwenk, F., Rajewsky, K. (1997). The Roles of $gamma$ 1 Heavy Chain Membrane Expression and Cytoplasmic Tail in IgG1 Responses. Science 276: 412-415 [Abstract] [Full Text]
Klein, U., Kuppers, R., Rajewsky, K. (1997). Evidence for a Large Compartment of IgM-Expressing Memory B Cells in Humans. Blood 89: 1288-1298 [Abstract] [Full Text]
Tumas-Brundage, K., Manser, T. (1997). The Transcriptional Promoter Regulates Hypermutation of the Antibody Heavy Chain Locus. JEM 185: 239-250 [Abstract] [Full Text]