Phagocytosis of tumor cells by human monocytes cultured in recombinant macrophage colony-stimulating factor

doi:10.1084/jem.172.1.231

This Article

	Full Text (PDF, 848K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Munn, D. H.
	Articles by Cheung, N. K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Munn, D. H.
	Articles by Cheung, N. K.


Social Bookmarking

	What's this?

ARTICLES

Phagocytosis of tumor cells by human monocytes cultured in recombinant macrophage colony-stimulating factor

DH Munn and NK Cheung
Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Macrophages and cultured human monocytes can mediate efficient antibody- dependent cytotoxicity (ADCC) against human tumor cells using monoclonal antibodies (mAbs). The mechanism of this killing is usually assumed to involve secreted factors (reactive oxygen intermediates, tumor necrosis factor, or other cytotoxic factors) leading to target cell lysis. In this study, we present evidence that phagocytosis of intact target cells is the principal mechanism of antitumor cytotoxicity in our in vitro model of ADCC by cultured monocytes. Human monocytes cultured in recombinant human macrophage colony-stimulating factor ingested up to 100% of fluorochrome-labeled melanoma and neuroblastoma target cells, in the presence of an appropriate antitumor mAb. Electron microscopy demonstrated phagocytosis of intact tumor cells by cultured monocytes during ADCC. All of the radionuclide in radiolabeled target cells was taken up by monocytes during phagocytosis. By preventing the release of radioisotope tracers, phagocytosis thus prevents the detection of this very efficient form of cytotoxicity by most conventional assays.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gordon, I. O., Freedman, R. S. (2006). Defective Antitumor Function of Monocyte-Derived Macrophages from Epithelial Ovarian Cancer Patients. Clin. Cancer Res. 12: 1515-1524 [Abstract] [Full Text]
Beum, P. V., Kennedy, A. D., Williams, M. E., Lindorfer, M. A., Taylor, R. P. (2006). The Shaving Reaction: Rituximab/CD20 Complexes Are Removed from Mantle Cell Lymphoma and Chronic Lymphocytic Leukemia Cells by THP-1 Monocytes. J. Immunol. 176: 2600-2609 [Abstract] [Full Text]
Cheung, I. Y., Lo Piccolo, M. S., Kushner, B. H., Kramer, K., Cheung, N.-K. V. (2003). Quantitation of GD2 Synthase mRNA by Real-Time Reverse Transcriptase Polymerase Chain Reaction: Clinical Utility in Evaluating Adjuvant Therapy in Neuroblastoma. JCO 21: 1087-1093 [Abstract] [Full Text]
Keler, T., Wallace, P. K., Vitale, L. A., Russoniello, C., Sundarapandiyan, K., Graziano, R. F., Deo, Y. M. (2000). Differential Effect of Cytokine Treatment on Fc{alpha} Receptor I- and Fc{gamma} Receptor I-Mediated Tumor Cytotoxicity by Monocyte-Derived Macrophages. J. Immunol. 164: 5746-5752 [Abstract] [Full Text]
Deo, Y. M., Sundarapandiyan, K., Keler, T., Wallace, P. K., Graziano, R. F. (1998). Bispecific Molecules Directed to the Fc Receptor for IgA (Fc{alpha}RI, CD89) and Tumor Antigens Efficiently Promote Cell-Mediated Cytotoxicity of Tumor Targets in Whole Blood. J. Immunol. 160: 1677-1686 [Abstract] [Full Text]
Lee, E., Grodzinsky, A. J., Libby, P., Clinton, S. K., Lark, M. W., Lee, R. T. (1995). Human Vascular Smooth Muscle Cell�Monocyte Interactions and Metalloproteinase Secretion in Culture. Arterioscler. Thromb. Vasc. Bio. 15: 2284-2289 [Abstract] [Full Text]