Evidence for extrathymic changes in the T cell receptor gamma/delta repertoire

doi:10.1084/jem.171.5.1597

This Article

	Full Text (PDF, 1202K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Parker, C. M.
	Articles by Brenner, M. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Parker, C. M.
	Articles by Brenner, M. B.


Social Bookmarking

	What's this?

ARTICLES

Evidence for extrathymic changes in the T cell receptor gamma/delta repertoire

CM Parker, V Groh, H Band, SA Porcelli, C Morita, M Fabbi, D Glass, JL Strominger and MB Brenner
Laboratory of Immunochemistry, Dana-Farber Cancer Institute, Boston, MA 02115.

The germline repertoire of variable genes for the TCR-gamma/delta is limited. This, together with the availability of several V delta- specific and a C delta-specific mAbs, has made it possible to assess differences in the TCR-gamma/delta repertoire in man. TCR-gamma/delta cells expressing particular V gene segments have been previously shown to be localized in different anatomical sites. In this study, analysis of TCR-gamma/delta V gene segment usage performed on subjects from the time of birth through adulthood revealed striking age-related changes in the TCR-gamma/delta repertoire in peripheral blood. V delta 1+ gamma/delta T cells predominated in thymus as well as in peripheral blood at birth and then persisted as a relatively constant proportion of CD3+ PBL. However, V delta 2+ gamma/delta T cells that constitute a small proportion of the CD3+ cells in thymus and in peripheral blood at birth, then expand and account for the major population of gamma/delta T cells in PBL in adults. No parallel postnatal expansion of V delta 2+ cells in the thymus was observed, even when paired thymus-peripheral blood specimens were obtained on subjects between the ages of 3 d and 8 yr. The subset of V delta 2+ lymphocytes that was expanded in peripheral blood expressed high levels of CD45RO suggesting prior activation of these cells, consistent with the possibility that their expansion might have resulted from exposure to foreign antigens or superantigens. In contrast, V delta 1+ T cells in PBL showed no comparable increase in relative numbers and were either negative or expressed only low levels of CD45RO. Consistent with evidence for extrathymic peripheral expansion of selective TCR-gamma/delta subsets, no link between MHC haplotype and differences in the TCR-gamma/delta V gene usage between individuals was apparent, and identical twins displayed TCR-gamma/delta variable gene segment phenotypes that were strikingly different from one another. The elements that determine the TCR-gamma/delta repertoire in individuals are not known. It is possible that both thymic selection and extrathymic factors may influence the peripheral repertoire. Recently, TCR-gamma/delta+ lymphocytes have been shown to expand markedly in peripheral lymphoid tissues and infectious lesions in response to mycobacterial antigens, and a correlation between mycobacterial responses and TCR-gamma/delta V gene usage has been shown in mice. The data presented here demonstrated peripheral age- related changes in the gamma/delta repertoire and point to the importance of extrathymic expansion of specific gamma/delta subsets in generating the human TCR-gamma/delta repertoire.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Dodd, J., Riffault, S., Kodituwakku, J. S., Hayday, A. C., Openshaw, P. J. M. (2009). Pulmonary V{gamma}4+ {gamma}{delta} T Cells Have Proinflammatory and Antiviral Effects in Viral Lung Disease. J. Immunol. 182: 1174-1181 [Abstract] [Full Text]
Pitard, V., Roumanes, D., Lafarge, X., Couzi, L., Garrigue, I., Lafon, M.-E., Merville, P., Moreau, J.-F., Dechanet-Merville, J. (2008). Long-term expansion of effector/memory V{delta}2- {gamma}{delta} T cells is a specific blood signature of CMV infection. Blood 112: 1317-1324 [Abstract] [Full Text]
Cummings, J.-S., Cairo, C., Armstrong, C., Davis, C. E., Pauza, C. D. (2008). Impacts of HIV infection on V{gamma}2V{delta}2 T cell phenotype and function: a mechanism for reduced tumor immunity in AIDS. J. Leukoc. Biol. 84: 371-379 [Abstract] [Full Text]
Li, L., Wu, C.-Y. (2008). CD4+CD25+ Treg cells inhibit human memory {gamma}{delta} T cells to produce IFN-{gamma} in response to M tuberculosis antigen ESAT-6. Blood 111: 5629-5636 [Abstract] [Full Text]
Kiladjian, J.-J., Visentin, G., Viey, E., Chevret, S., Eclache, V., Stirnemann, J., Bourhis, J. H., Chouaib, S., Fenaux, P., Caignard, A. (2008). Activation of cytotoxic T-cell receptor {gamma}{delta} T lymphocytes in response to specific stimulation in myelodysplastic syndromes. haematol 93: 381-389 [Abstract] [Full Text]
Puan, K.-J., Jin, C., Wang, H., Sarikonda, G., Raker, A. M., Lee, H. K., Samuelson, M. I., Marker-Hermann, E., Pasa-Tolic, L., Nieves, E., Giner, J.-L., Kuzuyama, T., Morita, C. T. (2007). Preferential recognition of a microbial metabolite by human V{gamma}2V{delta}2 T cells. Int Immunol 19: 657-673 [Abstract] [Full Text]
Tikhonov, I, Deetz, C., Paca, R, Berg, S, Lukyanenko, V, Lim, J., Pauza, C. (2006). Human V{gamma}2V{delta}2 T cells contain cytoplasmic RANTES. Int Immunol 18: 1243-1251 [Abstract] [Full Text]
Caccamo, N., Dieli, F., Wesch, D., Jomaa, H., Eberl, M. (2006). Sex-specific phenotypical and functional differences in peripheral human V{gamma}9/V{delta}2 T cells. J. Leukoc. Biol. 79: 663-666 [Abstract] [Full Text]
Angelini, D. F., Borsellino, G., Poupot, M., Diamantini, A., Poupot, R., Bernardi, G., Poccia, F., Fournie, J.-J., Battistini, L. (2004). Fc{gamma}RIII discriminates between 2 subsets of V{gamma}9V{delta}2 effector cells with different responses and activation pathways. Blood 104: 1801-1807 [Abstract] [Full Text]
Couedel, C., Lippert, E., Bernardeau, K., Bonneville, M., Davodeau, F. (2004). Allelic Exclusion at the TCR{delta} Locus and Commitment to {gamma}{delta} Lineage: Different Modalities Apply to Distinct Human {gamma}{delta} Subsets. J. Immunol. 172: 5544-5552 [Abstract] [Full Text]
Lee, J., Choi, K., Olin, M. R., Cho, S.-N., Molitor, T. W. (2004). {gamma}{delta} T Cells in Immunity Induced by Mycobacterium bovis Bacillus Calmette-Guerin Vaccination. Infect. Immun. 72: 1504-1511 [Abstract] [Full Text]
De Rosa, S. C., Andrus, J. P., Perfetto, S. P., Mantovani, J. J., Herzenberg, L. A., Herzenberg, L. A., Roederer, M. (2004). Ontogeny of {gamma}{delta} T Cells in Humans. J. Immunol. 172: 1637-1645 [Abstract] [Full Text]
Ramsburg, E., Tigelaar, R., Craft, J., Hayday, A. (2003). Age-dependent Requirement for {gamma}{delta} T Cells in the Primary but Not Secondary Protective Immune Response against an Intestinal Parasite. JEM 198: 1403-1414 [Abstract] [Full Text]
Yamashita, S., Tanaka, Y., Harazaki, M., Mikami, B., Minato, N. (2003). Recognition mechanism of non-peptide antigens by human {gamma}{delta} T cells. Int Immunol 15: 1301-1307 [Abstract] [Full Text]
Dieli, F., Poccia, F., Lipp, M., Sireci, G., Caccamo, N., Di Sano, C., Salerno, A. (2003). Differentiation of Effector/Memory V{delta}2 T Cells and Migratory Routes in Lymph Nodes or Inflammatory Sites. JEM 198: 391-397 [Abstract] [Full Text]
Krejci, O., Prouzova, Z., Horvath, O., Trka, J., Hrusak, O. (2003). Cutting Edge: TCR {delta} Gene Is Frequently Rearranged in Adult B Lymphocytes. J. Immunol. 171: 524-527 [Abstract] [Full Text]
Oyoshi, M. K., Nagata, H., Kimura, N., Zhang, Y., Demachi, A., Hara, T., Kanegane, H., Matsuo, Y., Yamaguchi, T., Morio, T., Hirano, A., Shimizu, N., Yamamoto, K. (2003). Preferential Expansion of V{gamma}9-J{gamma}P/V{delta}2-J{delta}3 {gamma}{delta} T Cells in Nasal T-Cell Lymphoma and Chronic Active Epstein-Barr Virus Infection. Am. J. Pathol. 162: 1629-1638 [Abstract] [Full Text]
Wang, H., Lee, H. K., Bukowski, J. F., Li, H., Mariuzza, R. A., Chen, Z. W., Nam, K.-H., Morita, C. T. (2003). Conservation of Nonpeptide Antigen Recognition by Rhesus Monkey V{gamma}2V{delta}2 T Cells. J. Immunol. 170: 3696-3706 [Abstract] [Full Text]
Van Rhijn, I., Van den Berg, L. H., Ang, C. W., Admiraal, J., Logtenberg, T. (2003). Expansion of human {gamma}{delta} T cells after in vitro stimulation with Campylobacter jejuni. Int Immunol 15: 373-382 [Abstract] [Full Text]
Dascher, C. C., Hiromatsu, K., Xiong, X., Sugita, M., Buhlmann, J. E., Dodge, I. L., Lee, S. Y., Roura-Mir, C., Watts, G. F., Roy, C. J., Behar, S. M., Clemens, D. L., Porcelli, S. A., Brenner, M. B. (2002). Conservation of CD1 Intracellular Trafficking Patterns Between Mammalian Species. J. Immunol. 169: 6951-6958 [Abstract] [Full Text]
Gurney, K. B., Yang, O. O., Wilson, S. B., Uittenbogaart, C. H. (2002). TCR{gamma}{delta}+ and CD161+ Thymocytes Express HIV-1 in the SCID-hu Mouse, Potentially Contributing to Immune Dysfunction in HIV Infection. J. Immunol. 169: 5338-5346 [Abstract] [Full Text]
Argentati, K., Re, F., Donnini, A., Tucci, M. G., Franceschi, C., Bartozzi, B., Bernardini, G., Provinciali, M. (2002). Numerical and functional alterations of circulating {gamma}{delta} T lymphocytes in aged people and centenarians. J. Leukoc. Biol. 72: 65-71 [Abstract] [Full Text]
Glatzel, A., Wesch, D., Schiemann, F., Brandt, E., Janssen, O., Kabelitz, D. (2002). Patterns of Chemokine Receptor Expression on Peripheral Blood {gamma}{delta} T Lymphocytes: Strong Expression of CCR5 Is a Selective Feature of V{delta}2/V{gamma}9 {gamma}{delta} T Cells. J. Immunol. 168: 4920-4929 [Abstract] [Full Text]
Tirouvanziam, R., Khazaal, I., N'Sonde, V., Peyrat, M.-A., Lim, A., de Bentzmann, S., Fournie, J. J., Bonneville, M., Peault, B. (2002). Ex vivo development of functional human lymph node and bronchus-associated lymphoid tissue. Blood 99: 2483-2489 [Abstract] [Full Text]
Barakonyi, A., Kovacs, K. T., Miko, E., Szereday, L., Varga, P., Szekeres-Bartho, J. (2002). Recognition of Nonclassical HLA Class I Antigens by {gamma}{delta} T Cells During Pregnancy. J. Immunol. 168: 2683-2688 [Abstract] [Full Text]
Gioia, C., Agrati, C., Casetti, R., Cairo, C., Borsellino, G., Battistini, L., Mancino, G., Goletti, D., Colizzi, V., Pucillo, L. P., Poccia, F. (2002). Lack of CD27-CD45RA-V{gamma}9V{delta}2+ T Cell Effectors in Immunocompromised Hosts and During Active Pulmonary Tuberculosis. J. Immunol. 168: 1484-1489 [Abstract] [Full Text]
Miyagawa, F., Tanaka, Y., Yamashita, S., Mikami, B., Danno, K., Uehara, M., Minato, N. (2001). Essential Contribution of Germline-Encoded Lysine Residues in J{gamma}1.2 Segment to the Recognition of Nonpeptide Antigens by Human {gamma}{delta} T Cells. J. Immunol. 167: 6773-6779 [Abstract] [Full Text]
Naiman, B. M., Alt, D., Bolin, C. A., Zuerner, R., Baldwin, C. L. (2001). Protective Killed Leptospira borgpetersenii Vaccine Induces Potent Th1 Immunity Comprising Responses by CD4 and gamma delta T Lymphocytes. Infect. Immun. 69: 7550-7558 [Abstract] [Full Text]
Morita, C. T., Lee, H. K., Wang, H., Li, H., Mariuzza, R. A., Tanaka, Y. (2001). Structural Features of Nonpeptide Prenyl Pyrophosphates That Determine Their Antigenicity for Human {{gamma}}{{delta}} T Cells. J. Immunol. 167: 36-41 [Abstract] [Full Text]
MacDougall, A. V., Enders, P., Hatfield, G., Pauza, D. C., Rakasz, E. (2001). V{{gamma}}2 TCR Repertoire Overlap in Different Anatomical Compartments of Healthy, Unrelated Rhesus Macaques. J. Immunol. 166: 2296-2302 [Abstract] [Full Text]
Hviid, L., Akanmori, B. D., Loizon, S., Kurtzhals, J. A. L., Ricke, C. H., Lim, A., Koram, K. A., Nkrumah, F. K., Mercereau-Puijalon, O., Behr, C. (2000). High frequency of circulating {gamma}{delta} T cells with dominance of the V{delta}1 subset in a healthy population. Int Immunol 12: 797-805 [Abstract] [Full Text]
Dascher, C. C., Hiromatsu, K., Naylor, J. W., Brauer, P. P., Brown, K. A., Storey, J. R., Behar, S. M., Kawasaki, E. S., Porcelli, S. A., Brenner, M. B., LeClair6, K. P. (1999). Conservation of a CD1 Multigene Family in the Guinea Pig. J. Immunol. 163: 5478-5488 [Abstract] [Full Text]
Belmant, C., Espinosa, E., Poupot, R., Peyrat, M.-A., Guiraud, M., Poquet, Y., Bonneville, M., Fournie, J.-J. (1999). 3-Formyl-1-butyl Pyrophosphate A Novel Mycobacterial Metabolite-activating Human gamma delta T Cells. J. Biol. Chem. 274: 32079-32084 [Abstract] [Full Text]
Shieh, C.-C., Sadasivan, B. K., Russell, G. J., Schon, M. P., Parker, C. M., Brenner, M. B. (1999). Lymphocyte Adhesion to Epithelia and Endothelia Mediated by the Lymphocyte Endothelial-Epithelial Cell Adhesion Molecule Glycoprotein. J. Immunol. 163: 1592-1601 [Abstract] [Full Text]
Groh, V., Rhinehart, R., Secrist, H., Bauer, S., Grabstein, K. H., Spies, T. (1999). Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc. Natl. Acad. Sci. USA 96: 6879-6884 [Abstract] [Full Text]
Schmidt, S. (1999). Candidate autoantigens in multiple sclerosis. Mult Scler 5: 147-160 [Abstract]
Bukowski, J. F., Morita, C. T., Band, H., Brenner, M. B. (1998). Crucial Role of TCR{gamma} Chain Junctional Region in Prenyl Pyrophosphate Antigen Recognition by {gamma}{delta} T Cells. J. Immunol. 161: 286-293 [Abstract] [Full Text]
McVay, L. D., Jaswal, S. S., Kennedy, C., Hayday, A., Carding, S. R. (1998). The Generation of Human {gamma}{delta} T Cell Repertoires During Fetal Development. J. Immunol. 160: 5851-5860 [Abstract] [Full Text]
Shen, J., Andrews, D. M., Pandolfi, F., Boyle, L. A., Kersten, C. M., Blatman, R. N., Kurnick, J. T. (1998). Oligoclonality of V{delta}1 and V{delta}2 Cells in Human Peripheral Blood Mononuclear Cells: TCR Selection Is Not Altered by Stimulation with Gram-Negative Bacteria. J. Immunol. 160: 3048-3055 [Abstract] [Full Text]
Higgins, J. M.G., Mandlebrot, D. A., Shaw, S. K., Russell, G. J., Murphy, E. A., Chen, Y.-T., Nelson, W. J., Parker, C. M., Brenner, M. B. (1998). Direct and Regulated Interaction of Integrin {alpha}E{beta}7 with E-Cadherin. JCB 140: 197-210 [Abstract] [Full Text]
Felsburg, P. J., Somberg, R. L., Hartnett, B. J., Suter, S. F., Henthorn, P. S., Moore, P. F., Weinberg, K. I., Ochs, H. D. (1997). Full Immunologic Reconstitution Following Nonconditioned Bone Marrow Transplantation for Canine X-Linked Severe Combined Immunodeficiency. Blood 90: 3214-3221 [Abstract] [Full Text]
Murakami, S., Okada, H. (1997). Lymphocyte-Fibroblast Interactions. CROBM 8: 40-50 [Abstract] [Full Text]
Vincent, M. S., Roessner, K., Lynch, D., Wilson, D., Cooper, S. M., Tschopp, J., Sigal, L. H., Budd, R. C. (1996). Apoptosis of Fashigh CD4+ Synovial T Cells by Borrelia-reactive Fas-ligandhigh {gamma}{delta} T Cells in Lyme Arthritis. JEM 184: 2109-2118 [Abstract] [Full Text]
Fisch, P, Malkovsky, M, Kovats, S, Sturm, E, Braakman, E, Klein, B., Voss, S., Morrissey, L., DeMars, R, Welch, W., et, al. (1990). Recognition by human V gamma 9/V delta 2 T cells of a GroEL homolog on Daudi Burkitt's lymphoma cells. Science 250: 1269-1273 [Abstract]
Lafont, V., Liautard, J., Sable-Teychene, M., Sainte-Marie, Y., Favero, J. (2001). Isopentenyl Pyrophosphate, a Mycobacterial Non-peptidic Antigen, Triggers Delayed and Highly Sustained Signaling in Human gamma delta T Lymphocytes without Inducing Down-modulation of T Cell Antigen Receptor. J. Biol. Chem. 276: 15961-15967 [Abstract] [Full Text]