The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 171, 1443-1452, Copyright © 1990 by Rockefeller University Press

ARTICLES

Preferential nuclear compartmentalization of endogenous mink cell focus- forming-related retroviral transcripts

MF Gourley, AM Krieg and AD Steinberg
Cellular Immunology Section, National Institute of Arthritis, Musculoskeletal and Skin Disease, National Institutes of Health, Bethesda, Maryland 20892.

Endogenous mink cell focus-forming (MCF)-like retroviral sequences in the murine genome are stable, inherited sequences analogous to other chromosomal genes. As such, it is thought that they are transcribed and translated in a manner analogous to other genes. However, when the SL12.4 CD4-, CD8- thymoma cell line was studied for nuclear/cytoplasmic distribution of endogenous MCF-related transcripts, there was a nuclear predominance. The great majority of full-length 8.4-kb endogenous MCF- related transcripts were nuclear. Even the smaller, spliced 3.0-kb transcripts were at least as prominent in the nucleus as the cytoplasm, whereas cellular RNA was 80% cytoplasmic and other cellular transcripts were represented in the cytoplasm to a much greater extent than the nucleus. Size cannot fully account for the nuclear presence of MCF- related endogenous transcripts, because the 3.0-kb MCF transcripts occurred in the nucleus to a much greater relative extent than 3.8-kb c- myb transcripts. These studies point to retroviral-like structures of these transcripts as influencing their intracellular compartmentalization.
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