A mycobacterial 65-kD heat shock protein induces antigen-specific suppression of adjuvant arthritis, but is not itself arthritogenic

doi:10.1084/jem.171.1.339

This Article

	Full Text (PDF, 396K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Billingham, M. E.
	Articles by Colston, M. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Billingham, M. E.
	Articles by Colston, M. J.


Social Bookmarking

	What's this?

ARTICLES

A mycobacterial 65-kD heat shock protein induces antigen-specific suppression of adjuvant arthritis, but is not itself arthritogenic

ME Billingham, S Carney, R Butler and MJ Colston
Connective Tissue Disease Research, Eli Lilly and Co., Windlesham, Surrey, United Kingdom.

A recombinant (r)65-kD protein from Mycobacterium leprae, at levels far in excess of those present in whole mycobacteria, was unable to induce arthritis. Even when combined with a synthetic adjuvant, CP20961, to mimic the peptidoglycan adjuvant component of the mycobacterial cell wall, the r65-kD protein failed to induce arthritis. Pretreatment with as little as 1 microgram r65-kD protein protected rats against arthritis induced by M. tuberculosis, but this r65-kD protein was markedly less able to protect against arthritis induced by the synthetic adjuvant, CP20961, or type II collagen. The r65-kD protein appears, therefore, to produce an antigen-specific protection against arthritis induced by bacterial cell walls containing the 65-kD protein. Such protection can be overcome, however, by arthritogenic T lymphocytes, suggesting that protection occurs by preventing clonal proliferation of autoreactive T lymphocytes that are induced by the adjuvant properties of mycobacterial cell walls. How the r65-kD protein abrogates this particular adjuvant activity, and the nature of the arthritogenic self antigen(s), remain to be elucidated.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Cohen-Sfady, M., Nussbaum, G., Pevsner-Fischer, M., Mor, F., Carmi, P., Zanin-Zhorov, A., Lider, O., Cohen, I. R. (2005). Heat Shock Protein 60 Activates B Cells via the TLR4-MyD88 Pathway. J. Immunol. 175: 3594-3602 [Abstract] [Full Text]
Lillicrap, M. S., Duggleby, R. C., Goodall, J. C., Gaston, J. S. H. (2004). T cell recognition of a highly conserved epitope in heat shock protein 60: self-tolerance maintained by TCR distinguishing between asparagine and aspartic acid. Int Immunol 16: 405-414 [Abstract] [Full Text]
Durai, M., Gupta, R. S., Moudgil, K. D. (2004). The T Cells Specific for the Carboxyl-Terminal Determinants of Self (Rat) Heat-Shock Protein 65 Escape Tolerance Induction and Are Involved in Regulation of Autoimmune Arthritis. J. Immunol. 172: 2795-2802 [Abstract] [Full Text]
Quintana, F. J., Carmi, P., Mor, F., Cohen, I. R. (2003). DNA Fragments of the Human 60-kDa Heat Shock Protein (HSP60) Vaccinate Against Adjuvant Arthritis: Identification of a Regulatory HSP60 Peptide. J. Immunol. 171: 3533-3541 [Abstract] [Full Text]
Ulmansky, R., Cohen, C. J., Szafer, F., Moallem, E., Fridlender, Z. G., Kashi, Y., Naparstek, Y. (2002). Resistance to Adjuvant Arthritis Is Due to Protective Antibodies Against Heat Shock Protein Surface Epitopes and the Induction of IL-10 Secretion. J. Immunol. 168: 6463-6469 [Abstract] [Full Text]
Pockley, A. G. (2002). Heat Shock Proteins, Inflammation, and Cardiovascular Disease. Circulation 105: 1012-1017 [Full Text]
Prakken, B. J., Wendling, U., van der Zee, R., Rutten, V. P. M. G., Kuis, W., van Eden, W. (2001). Induction of IL-10 and Inhibition of Experimental Arthritis Are Specific Features of Microbial Heat Shock Proteins That Are Absent for Other Evolutionarily Conserved Immunodominant Proteins. J. Immunol. 167: 4147-4153 [Abstract] [Full Text]
Adachi, N., Matsumoto, S., Tokuhisa, M., Kobayashi, K., Yamada, T. (2000). Antibodies against Mycobacterial Antigens in the Synovial Fluid of Patients with Temporomandibular Disorders. JDR 79: 1752-1757 [Abstract]
Paul, A. G. A., van der Zee, R., Taams, L. S., van Eden, W. (2000). A self-hsp60 peptide acts as a partial agonist inducing expression of B7-2 on mycobacterial hsp60-specific T cells: a possible mechanism for inhibitory T cell regulation of adjuvant arthritis?. Int Immunol 12: 1041-1050 [Abstract] [Full Text]
Wendling, U., Paul, L., van der Zee, R., Prakken, B., Singh, M., van Eden, W. (2000). A Conserved Mycobacterial Heat Shock Protein (hsp) 70 Sequence Prevents Adjuvant Arthritis upon Nasal Administration and Induces IL-10-Producing T Cells That Cross-React with the Mammalian Self-hsp70 Homologue. J. Immunol. 164: 2711-2717 [Abstract] [Full Text]
Tanaka, S., Kimura, Y., Mitani, A., Yamamoto, G., Nishimura, H., Spallek, R., Singh, M., Noguchi, T., Yoshikai, Y. (1999). Activation of T Cells Recognizing an Epitope of Heat-Shock Protein 70 Can Protect Against Rat Adjuvant Arthritis. J. Immunol. 163: 5560-5565 [Abstract] [Full Text]
Zugel, U., Kaufmann, S. H.�E. (1999). Role of Heat Shock Proteins in Protection from and Pathogenesis of Infectious Diseases. Clin. Microbiol. Rev. 12: 19-39 [Abstract] [Full Text]
Moudgil, K. D., Chang, T. T., Eradat, H., Chen, A. M., Gupta, R. S., Brahn, E., Sercarz, E. E. (1997). Diversification of T Cell Responses to Carboxy-terminal Determinants within the 65-kD Heat-shock Protein Is Involved in Regulation of Autoimmune Arthritis. JEM 185: 1307-1316 [Abstract] [Full Text]
Falcone, M., Bloom, B. R. (1997). A T Helper Cell 2 (Th2) Immune Response against Non-self Antigens Modifies the Cytokine Profile of Autoimmune T Cells and Protects against Experimental Allergic Encephalomyelitis. JEM 185: 901-908 [Abstract] [Full Text]