Journal of Experimental Medicine, Vol 170, 1559-1567, Copyright © 1989 by Rockefeller University Press

ARTICLES

Regulation of MHC class II gene expression in macrophages by hematopoietic colony-stimulating factors (CSF). Induction by granulocyte/macrophage CSF and inhibition by CSF-1

CL Willman, CC Stewart, V Miller, TL Yi and TB Tomasi
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.

CSF-1 and granulocyte/monocyte CSF (GM-CSF) were shown to modulate the levels of Ia gene and protein expression in bone marrow-derived macrophages (BMM). Recombinant GM-CSF induced high levels of Ia expression, similar to the levels induced by INF-gamma, while IL-3 had no effect. In contrast, recombinant CSF-1 not only suppressed the basal levels of Ia gene and protein expression in BMM, but also inhibited the induction of Ia by IFN-gamma and GM-CSF. Basal levels of Ia were not inhibited by recombinant CSF-1 until after 16-24 h of culture, suggesting an indirect mechanism of suppression. IFN-alpha/beta and PGE2 were shown not to be involved in the CSF-1 inhibition of basal levels of Ia expression. However, the CSF-1-mediated suppression of both the basal levels of Ia expression and the induction of Ia in BMM by IFN-gamma and GM-CSF did correlate with the induction of cellular proliferation. These data imply that in addition to regulating hematopoiesis, CSFs may regulate the initiation of the immune response through their effects on Ia expression in macrophages.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Fleetwood, A. J., Lawrence, T., Hamilton, J. A., Cook, A. D. (2007). Granulocyte-Macrophage Colony-Stimulating Factor (CSF) and Macrophage CSF-Dependent Macrophage Phenotypes Display Differences in Cytokine Profiles and Transcription Factor Activities: Implications for CSF Blockade in Inflammation. J. Immunol. 178: 5245-5252 [Abstract] [Full Text]  
• Rovida, E., Paccagnini, A., Del Rosso, M., Peschon, J., Dello Sbarba, P. (2001). TNF-{{alpha}}-Converting Enzyme Cleaves the Macrophage Colony-Stimulating Factor Receptor in Macrophages Undergoing Activation. J. Immunol. 166: 1583-1589 [Abstract] [Full Text]  
• Baron, C, Raposo, G, Scholl, S., Bausinger, H, Tenza, D, Bohbot, A, Pouillart, P, Goud, B, Hanau, D, Salamero, J (2001). Modulation of MHC class II transport and lysosome distribution by macrophage-colony stimulating factor in human dendritic cells derived from monocytes. J. Cell Sci. 114: 999-1010 [Abstract]  
• Xaus, J., Comalada, M., Barrachina, M., Herrero, C., Gonalons, E., Soler, C., Lloberas, J., Celada, A. (2000). The Expression of MHC Class II Genes in Macrophages Is Cell Cycle Dependent. J. Immunol. 165: 6364-6371 [Abstract] [Full Text]  
• Sester, D. P., Beasley, S. J., Sweet, M. J., Fowles, L. F., Cronau, S. L., Stacey, K. J., Hume, D. A. (1999). Bacterial/CpG DNA Down-Modulates Colony Stimulating Factor-1 Receptor Surface Expression on Murine Bone Marrow-Derived Macrophages with Concomitant Growth Arrest and Factor-Independent Survival. J. Immunol. 163: 6541-6550 [Abstract] [Full Text]  
• Horn, T. D., Rest, E. B., Karp, J. E., Burke, P. J., Vogelsang, G. B., Boucher, C. L., Hood, A. F. (1991). Interleukin 2 and Granulocyte-Macrophage Colony-Stimulating Factor Induce a Perivascular Lymphocytic Infiltrate in a Skin Explant Model. Arch Dermatol 127: 1789-1793 [Abstract]  
• Sinha, A., Lopez, M., McDevitt, H. (1990). Autoimmune diseases: the failure of self tolerance. Science 248: 1380-1388 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS