Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes

doi:10.1084/jem.170.4.1369

This Article

	Full Text (PDF, 1318K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Brooks, D. G.
	Articles by Ravetch, J. V.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Brooks, D. G.
	Articles by Ravetch, J. V.


Social Bookmarking

	What's this?

ARTICLES

Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes

DG Brooks, WQ Qiu, AD Luster and JV Ravetch
DeWitt Wallace Research Laboratory, Sloan-Kettering Institute, New York, New York 10021.

The structural heterogeneity of the human low affinity receptor for IgG, FcRII(CD32), has been elucidated through the isolation, characterization, and expression of cDNA clones derived from myeloid and lymphoid RNA. These clones predict amino acid sequences consistent with integral membrane glycoproteins with single membrane spanning domains. The extracellular domains display sequence homology to other Fc gamma Rs and members of the Ig supergene family. A minimum of three genes (Fc gamma RIIa, IIa', and Fc gamma RIIb) encode these transcripts, which demonstrate highly related extracellular and membrane spanning domains. IIa/IIa' differ substantially in the intracytoplasmic domain from IIb. Alternative splicing of the IIb gene generates further heterogeneity in both NH2- and COOH-terminal domains of the predicted proteins. Comparison to the murine homologues of these molecules reveals a high degree of conservation between the products of one of these genes, Fc gamma RIIb, and the murine beta gene in primary sequence, splicing pattern, and tissue distribution. In contrast, the sequence of IIa' indicates its relationship to the beta-like genes, with mutation giving rise to a novel cytoplasmic domain, while IIa is a chimera of both alpha- and beta-like genes. Expression of these cDNA molecules by transfection results in the appearance of IgG binding molecules that bear the epitopes defined by the FcRII(CD32) mAbs previously described.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Dutertre, C.-A., Bonnin-Gelize, E., Pulford, K., Bourel, D., Fridman, W.-H., Teillaud, J.-L. (2008). A novel subset of NK cells expressing high levels of inhibitory Fc{gamma}RIIB modulating antibody-dependent function. J. Leukoc. Biol. 84: 1511-1520 [Abstract] [Full Text]
Boylan, B., Gao, C., Rathore, V., Gill, J. C., Newman, D. K., Newman, P. J. (2008). Identification of Fc{gamma}RIIa as the ITAM-bearing receptor mediating {alpha}IIb{beta}3 outside-in integrin signaling in human platelets. Blood 112: 2780-2786 [Abstract] [Full Text]
Kamen, L. A., Levinsohn, J., Swanson, J. A. (2007). Differential Association of Phosphatidylinositol 3-Kinase, SHIP-1, and PTEN with Forming Phagosomes. Mol. Biol. Cell 18: 2463-2472 [Abstract] [Full Text]
Rankin, C. T., Veri, M.-C., Gorlatov, S., Tuaillon, N., Burke, S., Huang, L., Inzunza, H. D., Li, H., Thomas, S., Johnson, S., Stavenhagen, J., Koenig, S., Bonvini, E. (2006). CD32B, the human inhibitory Fc-{gamma} receptor IIB, as a target for monoclonal antibody therapy of B-cell lymphoma. Blood 108: 2384-2391 [Abstract] [Full Text]
Mackay, M., Stanevsky, A., Wang, T., Aranow, C., Li, M., Koenig, S., Ravetch, J. V., Diamond, B. (2006). Selective dysregulation of the Fc{gamma}IIB receptor on memory B cells in SLE. JEM 203: 2157-2164 [Abstract] [Full Text]
Guriec, N., Daniel, C., Le Ster, K., Hardy, E., Berthou, C. (2006). Cytokine-regulated expression and inhibitory function of Fc{gamma}RIIB1 and -B2 receptors in human dendritic cells. J. Leukoc. Biol. 79: 59-70 [Abstract] [Full Text]
Liu, Y., Masuda, E., Blank, M. C., Kirou, K. A., Gao, X., Park, M.-S., Pricop, L. (2005). Cytokine-mediated regulation of activating and inhibitory Fc{gamma} receptors in human monocytes. J. Leukoc. Biol. 77: 767-776 [Abstract] [Full Text]
Scott, C., Higgins, M. E., Davies, J. P., Ioannou, Y. A. (2004). Targeting of NPC1 to Late Endosomes Involves Multiple Signals, Including One Residing within the Putative Sterol-sensing Domain. J. Biol. Chem. 279: 48214-48223 [Abstract] [Full Text]
Marshall, A. S. J., Willment, J. A., Lin, H.-H., Williams, D. L., Gordon, S., Brown, G. D. (2004). Identification and Characterization of a Novel Human Myeloid Inhibitory C-type Lectin-like Receptor (MICL) That Is Predominantly Expressed on Granulocytes and Monocytes. J. Biol. Chem. 279: 14792-14802 [Abstract] [Full Text]
Garcia-Garcia, E., Rosales, C. (2002). Signal transduction during Fc receptor-mediated phagocytosis. J. Leukoc. Biol. 72: 1092-1108 [Abstract] [Full Text]
Furtado, P B, McElveen, J E, Gough, L, Armour, K L, Clark, M R, Sewell, H F, Shakib, F (2002). The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype. Mol. Pathol. 55: 315-324 [Abstract] [Full Text]
Wightman, J., Roberson, M. S., Lamkin, T. J., Varvayanis, S., Yen, A. (2002). Retinoic Acid-induced Growth Arrest and Differentiation: Retinoic Acid Up-Regulates CD32 (Fc{gamma}RII) Expression, the Ectopic Expression of Which Retards the Cell Cycle. Molecular Cancer Therapeutics 1: 493-506 [Abstract] [Full Text]
Barabe, F., Rollet-Labelle, E., Gilbert, C., Fernandes, M. J. G., Naccache, S. N., Naccache, P. H. (2002). Early Events in the Activation of Fc{gamma}RIIA in Human Neutrophils: Stimulated Insolubilization, Translocation to Detergent-Resistant Domains, and Degradation of Fc{gamma}RIIA. J. Immunol. 168: 4042-4049 [Abstract] [Full Text]
Tridandapani, S., Siefker, K., Teillaud, J.-L., Carter, J. E., Wewers, M. D., Anderson, C. L. (2002). Regulated Expression and Inhibitory Function of Fcgamma RIIb in Human Monocytic Cells. J. Biol. Chem. 277: 5082-5089 [Abstract] [Full Text]
Higgins, M. E., Ioannou, Y. A. (2001). Apoptosis-induced release of mature sterol regulatory element-binding proteins activates sterol-responsive genes. J. Lipid Res. 42: 1939-1946 [Abstract] [Full Text]
Nishimura, T., Narita, T., Miyazaki, E., Ito, T., Nishimoto, N., Yoshizaki, K., Martial, J. A., Bellfroid, E. J., Vissing, H., Taniyama, T. (2001). Characterization of the human Fc{gamma}RIIB gene promoter: human zinc-finger proteins (ZNF140 and ZNF91) that bind to different regions function as transcription repressors. Int Immunol 13: 1075-1084 [Abstract] [Full Text]
Pricop, L., Redecha, P., Teillaud, J.-L., Frey, J., Fridman, W. H., Sautes-Fridman, C., Salmon, J. E. (2001). Differential Modulation of Stimulatory and Inhibitory Fc{{gamma}} Receptors on Human Monocytes by Th1 and Th2 Cytokines. J. Immunol. 166: 531-537 [Abstract] [Full Text]
Willis, T. G., Dyer, M. J. S. (2000). The role of immunoglobulin translocations in the pathogenesis of B-cell malignancies. Blood 96: 808-822 [Full Text]
Cortegano, I., Pozo, V. d., Cardaba, B., Arrieta, I., Gallardo, S., Rojo, M., Aceituno, E., Takai, T., Verbeek, S., Palomino, P., Liu, F. T., Lahoz, C. (2000). Interaction between galectin-3 and Fc{gamma}RII induces down-regulation of IL-5 gene: implication of the promoter sequence IL-5REIII. Glycobiology 10: 237-242 [Abstract] [Full Text]
Callanan, M. B., Le Baccon, P., Mossuz, P., Duley, S., Bastard, C., Hamoudi, R., Dyer, M. J., Klobeck, G., Rimokh, R., Sotto, J. J., Leroux, D. (2000). The IgG Fc receptor, Fcgamma RIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma. Proc. Natl. Acad. Sci. USA 97: 309-314 [Abstract] [Full Text]
Huls, G., Heijnen, I. A. F. M., Cuomo, E., van der Linden, J., Boel, E., van de Winkel, J. G. J., Logtenberg, T. (1999). Antitumor Immune Effector Mechanisms Recruited by Phage Display-derived Fully Human IgG1 and IgA1 Monoclonal Antibodies. Cancer Res. 59: 5778-5784 [Abstract] [Full Text]
Giembycz, M. A., Lindsay, M. A. (1999). Pharmacology of the Eosinophil. Pharmacol. Rev. 51: 213-340 [Abstract] [Full Text]
Kim, J.-T., Schimming, A. W., Kita, H. (1999). Ligation of Fc{gamma}RII (CD32) Pivotally Regulates Survival of Human Eosinophils. J. Immunol. 162: 4253-4259 [Abstract] [Full Text]
Carlsson, L. E., Santoso, S., Baurichter, G., Kroll, H., Papenberg, S., Eichler, P., Westerdaal, N. A.C., Kiefel, V., van de Winkel, J. G.J., Greinacher, A. (1998). Heparin-Induced Thrombocytopenia: New Insights Into the Impact of the Fcgamma RIIa-R-H131 Polymorphism. Blood 92: 1526-1531 [Abstract] [Full Text]
Metes, D., Ernst, L. K., Chambers, W. H., Sulica, A., Herberman, R. B., Morel, P. A. (1998). Expression of Functional CD32 Molecules on Human NK Cells Is Determined by an Allelic Polymorphism of the Fcgamma RIIC Gene. Blood 91: 2369-2380 [Abstract] [Full Text]
Hunter, S., Indik, Z. K., Kim, M.-K., Cauley, M. D., Park, J.-G., Schreiber, A. D. (1998). Inhibition of Fcgamma Receptor-Mediated Phagocytosis by a Nonphagocytic Fcgamma Receptor. Blood 91: 1762-1768 [Abstract] [Full Text]
Floto, R. A., Somasundaram, B., Allen, J. M., Mahaut-Smith, M. P. (1997). Fcgamma Receptor I Activation Triggers a Novel Ca2+-activated Current Selective for Monovalent Cations in the Human Monocytic Cell Line, U937. J. Biol. Chem. 272: 4753-4758 [Abstract] [Full Text]
Chacko, G. W., Brandt, J. T., Coggeshall, K. M., Anderson, C. L. (1996). Phosphoinositide 3-Kinase and p72[IMAGE] Noncovalently Associate with the Low Affinity Fc[IMAGE] Receptor on Human Platelets through an Immunoreceptor Tyrosine-based Activation Motif. J. Biol. Chem. 271: 10775-10781 [Abstract] [Full Text]
Hulett, M. D., Witort, E., Brinkworth, R. I., McKenzie, I. F. C., Hogarth, P. M. (1995). Multiple Regions of Human Fc[IMAGE]RII (CD32) Contribute to the Binding of IgG. J. Biol. Chem. 270: 21188-21194 [Abstract] [Full Text]
Odin, J., Edberg, J., Painter, C., Kimberly, R., Unkeless, J. (1991). Regulation of phagocytosis and [Ca2+]i flux by distinct regions of an Fc receptor. Science 254: 1785-1788 [Abstract]
Qiu, W., de Bruin, D, Brownstein, B., Pearse, R, Ravetch, J. (1990). Organization of the human and mouse low-affinity Fc gamma R genes: duplication and recombination. Science 248: 732-735 [Abstract]
Kobayashi, N., Suzuki, Y., Tsuge, T., Okumura, K., Ra, C., Tomino, Y. (2002). FcRn-mediated transcytosis of immunoglobulin G in human renal proximal tubular epithelial cells. Am. J. Physiol. Renal Physiol. 282: F358-F365 [Abstract] [Full Text]