CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells

doi:10.1084/jem.170.3.637

This Article

	Full Text (PDF, 1226K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Davies, A.
	Articles by Waldmann, H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Davies, A.
	Articles by Waldmann, H.


Social Bookmarking

	What's this?

ARTICLES

CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells

A Davies, DL Simmons, G Hale, RA Harrison, H Tighe, PJ Lachmann and H Waldmann
MRC Molecular Immunopathology Unit, MRC Centre, Cambridge, United Kingdom.

A novel cell surface antigen has been identified on a wide range of lymphoid cells and erythrocytes. A mAb YTH 53.1 (CD59) against this antigen enhanced the lysis of human red cells and lymphocytes by homologous complement. Studies of reactive lysis using different species of C56, and of whole serum used as a source of C7-9, indicated that the inhibitory activity of the CD59 antigen is directed towards the homologous membrane attack complex. CD59 antigen was purified from human urine and erythrocyte stroma by affinity chromatography using the mAb YTH 53.1 immobilized on Sepharose, and, following transient expression of a human T cell cDNA library in COS cells, the corresponding cDNA also identified using the antibody. It was found that the CD59 antigen is a small protein (approximately 20 kD as judged by SDS-PAGE, 11.5 kD predicted from the isolated cDNA) sometimes associated with larger components (45 and 80 kD) in urine. The sequence of CD59 antigen is unlike that of other complement components or regulatory proteins, but shows 26% identity with that of the murine LY- 6 antigen. CD59 antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Koch, N., Jung, M., Sabat, R., Kratzschmar, J., Docke, W.-D., Asadullah, K., Volk, H.-D., Grutz, G. (2009). IL-10 protects monocytes and macrophages from complement-mediated lysis. J. Leukoc. Biol. 86: 155-166 [Abstract] [Full Text]
Hijazi, A., Masson, W., Auge, B., Waltzer, L., Haenlin, M., Roch, F. (2009). boudin is required for septate junction organisation in Drosophila and codes for a diffusible protein of the Ly6 superfamily. Development 136: 2199-2209 [Abstract] [Full Text]
Abbitt, K. B., Cotter, M. J., Ridger, V. C., Crossman, D. C., Hellewell, P. G., Norman, K. E. (2009). Antibody ligation of murine Ly-6G induces neutropenia, blood flow cessation, and death via complement-dependent and independent mechanisms. J. Leukoc. Biol. 85: 55-63 [Abstract] [Full Text]
Ramo, K., Cashman, S. M., Kumar-Singh, R. (2008). Evaluation of Adenovirus-Delivered Human CD59 as a Potential Therapy for AMD in a Model of Human Membrane Attack Complex Formation on Murine RPE. IOVS 49: 4126-4136 [Abstract] [Full Text]
Kinderlerer, A. R., Ali, F., Johns, M., Lidington, E. A., Leung, V., Boyle, J. J., Hamdulay, S. S., Evans, P. C., Haskard, D. O., Mason, J. C. (2008). KLF2-dependent, Shear Stress-induced Expression of CD59: A NOVEL CYTOPROTECTIVE MECHANISM AGAINST COMPLEMENT-MEDIATED INJURY IN THE VASCULATURE. J. Biol. Chem. 283: 14636-14644 [Abstract] [Full Text]
Goodman, S. R., Hughes, K. M. H., Kakhniashvili, D. G., Neelam, S. (2007). The Isolation of Reticulocyte-Free Human Red Blood Cells. Exp. Biol. Med. 232: 1470-1476 [Abstract] [Full Text]
Higuchi, M., Endo, Y., Suzuki, H., Osuka, F., Shio, Y., Fujiu, K., Kanno, R., Oishi, A., Fujita, T., Gotoh, M. (2006). Identification of the Decay-Accelerating Factor CD55 as a Peanut Agglutinin-Binding Protein and Its Alteration in Non-Small Cell Lung Cancers.. Clin. Cancer Res. 12: 6367-6372 [Abstract] [Full Text]
Frankland, S., Adisa, A., Horrocks, P., Taraschi, T. F., Schneider, T., Elliott, S. R., Rogerson, S. J., Knuepfer, E., Cowman, A. F., Newbold, C. I., Tilley, L. (2006). Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains. Eukaryot Cell 5: 849-860 [Abstract] [Full Text]
Spitzer, D., Unsinger, J., Mao, D., Wu, X., Molina, H., Atkinson, J. P. (2005). In Vivo Correction of Complement Regulatory Protein Deficiency with an Inhibitor Targeting the Red Blood Cell Membrane. J. Immunol. 175: 7763-7770 [Abstract] [Full Text]
Ragupathi, G., Liu, N. X., Musselli, C., Powell, S., Lloyd, K., Livingston, P. O. (2005). Antibodies against Tumor Cell Glycolipids and Proteins, but Not Mucins, Mediate Complement-Dependent Cytotoxicity. J. Immunol. 174: 5706-5712 [Abstract] [Full Text]
Olszewski, A. J., Grossbard, M. L. (2004). Empowering Targeted Therapy: Lessons from Rituximab. Sci Signal 2004: pe30-pe30 [Abstract] [Full Text]
Lin, F., Spencer, D., Hatala, D. A., Levine, A. D., Medof, M. E. (2004). Decay-Accelerating Factor Deficiency Increases Susceptibility to Dextran Sulfate Sodium-Induced Colitis: Role for Complement in Inflammatory Bowel Disease. J. Immunol. 172: 3836-3841 [Abstract] [Full Text]
Lin, F., Salant, D. J., Meyerson, H., Emancipator, S., Morgan, B. P., Medof, M. E. (2004). Respective Roles of Decay-Accelerating Factor and CD59 in Circumventing Glomerular Injury in Acute Nephrotoxic Serum Nephritis. J. Immunol. 172: 2636-2642 [Abstract] [Full Text]
Deng, J., Gold, D., LoVerde, P. T., Fishelson, Z. (2003). Inhibition of the Complement Membrane Attack Complex by Schistosoma mansoni Paramyosin. Infect. Immun. 71: 6402-6410 [Abstract] [Full Text]
Ghiran, I., Klickstein, L. B., Nicholson-Weller, A. (2003). Calreticulin Is at the Surface of Circulating Neutrophils and Uses CD59 as an Adaptor Molecule. J. Biol. Chem. 278: 21024-21031 [Abstract] [Full Text]
Pausa, M., Pellis, V., Cinco, M., Giulianini, P. G., Presani, G., Perticarari, S., Murgia, R., Tedesco, F. (2003). Serum-Resistant Strains of Borrelia burgdorferi Evade Complement-Mediated Killing by Expressing a CD59-Like Complement Inhibitory Molecule. J. Immunol. 170: 3214-3222 [Abstract] [Full Text]
Pflugh, D. L., Maher, S. E., Bothwell, A. L. M. (2002). Ly-6 Superfamily Members Ly-6A/E, Ly-6C, and Ly-6I Recognize Two Potential Ligands Expressed by B Lymphocytes. J. Immunol. 169: 5130-5136 [Abstract] [Full Text]
Tada, H., Sugawara, S., Nemoto, E., Takahashi, N., Imamura, T., Potempa, J., Travis, J., Shimauchi, H., Takada, H. (2002). Proteolysis of CD14 on Human Gingival Fibroblasts by Arginine-Specific Cysteine Proteinases from Porphyromonas gingivalis Leading to Down-Regulation of Lipopolysaccharide-Induced Interleukin-8 Production. Infect. Immun. 70: 3304-3307 [Abstract] [Full Text]
Wheeler, S. F., Rudd, P. M., Davis, S. J., Dwek, R. A., Harvey, D. J. (2002). Comparison of the N-linked glycans from soluble and GPI-anchored CD59 expressed in CHO cells. Glycobiology 12: 261-271 [Abstract] [Full Text]
Dormer, R. L., Derand, R., McNeilly, C. M., Mettey, Y., Bulteau-Pignoux, L., Metaye, T., Vierfond, J.-M., Gray, M. A., Galietta, L. J. V., Morris, M. R., Pereira, M. M. C., Doull, I. J. M., Becq, F., McPherson, M. A. (2002). Correction of delF508-CFTR activity with benzo(c)quinolizinium compounds through facilitation of its processing in cystic fibrosis airway cells. J. Cell Sci. 114: 4073-4081 [Abstract] [Full Text]
Farkas, I., Baranyi, L., Ishikawa, Y., Okada, N., Bohata, C., Budai, D., Fukuda, A., Imai, M., Okada, H. (2002). CD59 blocks not only the insertion of C9 into MAC but inhibits ion channel formation by homologous C5b-8 as well as C5b-9. J. Physiol. 539: 537-545 [Abstract] [Full Text]
Sogabe, H., Nangaku, M., Ishibashi, Y., Wada, T., Fujita, T., Sun, X., Miwa, T., Madaio, M. P., Song, W.-C. (2001). Increased Susceptibility of Decay-Accelerating Factor Deficient Mice to Anti-Glomerular Basement Membrane Glomerulonephritis. J. Immunol. 167: 2791-2797 [Abstract] [Full Text]
Weng, W.-K., Levy, R. (2001). Expression of complement inhibitors CD46, CD55, and CD59 on tumor cells does not predict clinical outcome after rituximab treatment in follicular non-Hodgkin lymphoma. Blood 98: 1352-1357 [Abstract] [Full Text]
Margos, G., Navarette, S., Butcher, G., Davies, A., Willers, C., Sinden, R. E., Lachmann, P. J. (2001). Interaction between Host Complement and Mosquito-Midgut-Stage Plasmodium berghei. Infect. Immun. 69: 5064-5071 [Abstract] [Full Text]
Chuntharapai, A., Dodge, K., Grimmer, K., Schroeder, K., Marsters, S. A., Koeppen, H., Ashkenazi, A., Kim, K. J. (2001). Isotype-Dependent Inhibition of Tumor Growth In Vivo by Monoclonal Antibodies to Death Receptor 4. J. Immunol. 166: 4891-4898 [Abstract] [Full Text]
Bulteau, L., Derand, R., Mettey, Y., Metaye, T., Morris, M. R., McNeilly, C. M., Folli, C., Galietta, L. J. V., Zegarra-Moran, O., Pereira, M. M. C., Jougla, C., Dormer, R. L., Vierfond, J.-M., Joffre, M., Becq, F. (2000). Properties of CFTR activated by the xanthine derivative X-33 in human airway Calu-3 cells. Am. J. Physiol. Cell Physiol. 279: C1925-C1937 [Abstract] [Full Text]
Lidington, E. A., Haskard, D. O., Mason, J. C. (2000). Induction of decay-accelerating factor by thrombin through a protease-activated receptor 1 and protein kinase C-dependent pathway protects vascular endothelial cells from complement-mediated injury. Blood 96: 2784-2792 [Abstract] [Full Text]
Singhrao, S. K., Neal, J. W., Rushmere, N. K., Morgan, B. P., Gasque, P. (2000). Spontaneous Classical Pathway Activation and Deficiency of Membrane Regulators Render Human Neurons Susceptible to Complement Lysis. Am. J. Pathol. 157: 905-918 [Abstract] [Full Text]
Qian, Y.-M., Qin, X., Miwa, T., Sun, X., Halperin, J. A., Song, W.-C. (2000). Identification and Functional Characterization of a New Gene Encoding the Mouse Terminal Complement Inhibitor CD59. J. Immunol. 165: 2528-2534 [Abstract] [Full Text]
Pflugh, D. L., Maher, S. E., Bothwell, A. L. M. (2000). Ly-6I, a New Member of the Murine Ly-6 Superfamily with a Distinct Pattern of Expression. J. Immunol. 165: 313-321 [Abstract] [Full Text]
Sugawara, S., Nemoto, E., Tada, H., Miyake, K., Imamura, T., Takada, H. (2000). Proteolysis of Human Monocyte CD14 by Cysteine Proteinases (Gingipains) from Porphyromonas gingivalis Leading to Lipopolysaccharide Hyporesponsiveness. J. Immunol. 165: 411-418 [Abstract] [Full Text]
Golay, J., Zaffaroni, L., Vaccari, T., Lazzari, M., Borleri, G.-M., Bernasconi, S., Tedesco, F., Rambaldi, A., Introna, M. (2000). Biologic response of B lymphoma cells to anti-CD20 monoclonal antibody rituximab in vitro: CD55 and CD59 regulate complement-mediated cell lysis. Blood 95: 3900-3908 [Abstract] [Full Text]
Junnikkala, S., Jokiranta, T. S., Friese, M. A., Jarva, H., Zipfel, P. F., Meri, S. (2000). Exceptional Resistance of Human H2 Glioblastoma Cells to Complement-Mediated Killing by Expression and Utilization of Factor H and Factor H-Like Protein 1. J. Immunol. 164: 6075-6081 [Abstract] [Full Text]
Acosta, J., Hettinga, J., Fluckiger, R., Krumrei, N., Goldfine, A., Angarita, L., Halperin, J. (2000). Molecular basis for a link between complement and the vascular complications of diabetes. Proc. Natl. Acad. Sci. USA 97: 5450-5455 [Abstract] [Full Text]
Varsano, S., Kaminsky, M., Kaiser, M., Rashkovsky, L. (2000). Generation of complement C3 and expression of cell membrane complement inhibitory proteins by human bronchial epithelium cell line. Thorax 55: 364-369 [Abstract] [Full Text]
Chen, S., Caragine, T., Cheung, N.-K. V., Tomlinson, S. (2000). Surface Antigen Expression and Complement Susceptibility of Differentiated Neuroblastoma Clones. Am. J. Pathol. 156: 1085-1091 [Abstract] [Full Text]
Young, N. S., Abkowitz, J. L., Luzzatto, L. (2000). New Insights into the Pathophysiology of Acquired Cytopenias. ASH Education Book 2000: 18-38 [Abstract] [Full Text]
Mason, J. C., Yarwood, H., Sugars, K., Morgan, B. P., Davies, K. A., Haskard, D. O. (1999). Induction of Decay-Accelerating Factor by Cytokines or the Membrane-Attack Complex Protects Vascular Endothelial Cells Against Complement Deposition. Blood 94: 1673-1682 [Abstract] [Full Text]
Tone, M., Diamond, L. E., Walsh, L. A., Tone, Y., Thompson, S. A.�J., Shanahan, E. M., Logan, J. S., Waldmann, H. (1999). High Level Transcription of the Complement Regulatory Protein CD59 Requires an Enhancer Located in Intron 1. J. Biol. Chem. 274: 710-716 [Abstract] [Full Text]
Murray, E. W., Robbins, S. M. (1998). Antibody Cross-linking of the Glycosylphosphatidylinositol-linked Protein CD59 on Hematopoietic Cells Induces Signaling Pathways Resembling Activation by Complement. J. Biol. Chem. 273: 25279-25284 [Abstract] [Full Text]
Varsano, S., Rashkovsky, L., Shapiro, H., Radnay, J. (1998). Cytokines Modulate Expression of Cell-Membrane Complement Inhibitory Proteins in Human Lung Cancer Cell Lines. Am. J. Respir. Cell Mol. Bio. 19: 522-529 [Abstract] [Full Text]
Hakulinen, J., Meri, S. (1998). Complement-Mediated Killing of Microtumors in Vitro. Am. J. Pathol. 153: 845-855 [Abstract] [Full Text]
Chen, R., Walter, E. I., Parker, G., Lapurga, J. P., Millan, J. L., Ikehara, Y., Udenfriend, S., Medof, M. E. (1998). Mammalian glycophosphatidylinositol anchor transfer to proteins and posttransfer deacylation. Proc. Natl. Acad. Sci. USA 95: 9512-9517 [Abstract] [Full Text]
Rautemaa, R., Jarvis, G. A., Marnila, P., Meri, S. (1998). Acquired Resistance of Escherichia coli to Complement Lysis by Binding of Glycophosphoinositol-Anchored Protectin (CD59). Infect. Immun. 66: 1928-1933 [Abstract] [Full Text]
Zhao, X.-j., Zhao, J., Zhou, Q., Sims, P. J. (1998). Identity of the Residues Responsible for the Species-restricted Complement Inhibitory Function of Human CD59. J. Biol. Chem. 273: 10665-10671 [Abstract] [Full Text]
Hinchliffe, S. J., Rushmere, N. K., Hanna, S. M., Morgan, B. P. (1998). Molecular Cloning and Functional Characterization of the Pig Analogue of CD59: Relevance to Xenotransplantation. J. Immunol. 160: 3924-3932 [Abstract] [Full Text]
Wang, G., Nonaka, M., He, C., Okada, N., Nakashima, I., Okada, H. (1998). Functional Differences Among Multiple Isoforms of Guinea Pig Decay-Accelerating Factor. J. Immunol. 160: 3014-3022 [Abstract] [Full Text]
Makrides, S. C. (1998). Therapeutic Inhibition of the Complement System. Pharmacol. Rev. 50: 59-88 [Abstract] [Full Text]
Shichishima, T., Saitoh, Y., Terasawa, T., Ogawa, K., Maruyama, Y. (1997). Relationship Between the Phenotypes of Circulating Erythrocytes and Cultured Erythroblasts in Paroxysmal Nocturnal Hemoglobinuria. Blood 90: 435-443 [Abstract] [Full Text]
Rudd, P. M., Morgan, B. P., Wormald, M. R., Harvey, D. J., van den Berg, C. W., Davis, S. J., Ferguson, M. A.J., Dwek, R. A. (1997). The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59. J. Biol. Chem. 272: 7229-7244 [Abstract] [Full Text]
Bodian, D. L., Davis, S. J., Morgan, B. P., Rushmere, N. K. (1997). Mutational Analysis of the Active Site and Antibody Epitopes of the Complement-inhibitory Glycoprotein, CD59. JEM 185: 507-516 [Abstract] [Full Text]
Bugge, T. H., Suh, T. T., Flick, M. J., Daugherty, C. C., R, J., Solberg, H., Ellis, V., Dan�, K., Degen, J. L. (1995). The Receptor for Urokinase-type Plasminogen Activator Is Not Essential for Mouse Development or Fertility. J. Biol. Chem. 270: 16886-16894 [Abstract] [Full Text]
Kooyman, D., Byrne, G., McClellan, S, Nielsen, D, Tone, M, Waldmann, H, Coffman, T., McCurry, K., Platt, J., Logan, J. (1995). In vivo transfer of GPI-linked complement restriction factors from erythrocytes to the endothelium. Science 269: 89-92 [Abstract]
Stahl, G. L., Reenstra, W. R., Frendl, G. (1995). Complement-Mediated Loss of Endothelium-Dependent Relaxation of Porcine Coronary Arteries : Role of the Terminal Membrane Attack Complex. Circ. Res. 76: 575-583 [Abstract] [Full Text]
Hahn, W., Menu, E, Bothwell, A., Sims, P., Bierer, B. (1992). Overlapping but nonidentical binding sites on CD2 for CD58 and a second ligand CD59. Science 256: 1805-1807 [Abstract]