Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody

doi:10.1084/jem.169.4.1333

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Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody

PR Crocker and S Gordon
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.

An inhibitory rat mAb, SER-4, has been raised to the mouse macrophage (M phi)-restricted hemagglutinin, sheep erythrocyte receptor (SER), which binds unopsonized sheep erythrocytes through recognition of sialylated glycoconjugates. This receptor was originally defined on mouse resident bone marrow M phi where it was implicated in adhesive interactions of these cells with proliferating hematopoietic cells. In the present study using mouse serum-induced thioglycollate-elicited peritoneal M phi (TPM) as a model system for SER expression, mAb SER-4 IgG2a completely blocked rosette formation at 1 microgram/ml. The inhibition was likely to be via steric hindrance rather than through a direct interaction with the putative sialic acid binding site of SER because F(ab')2 and Fab fragments of mAb SER-4 gave a maximum inhibition of 50-60% and 0% respectively, despite binding effectively to the SER-4 antigen (Ag). Immunoprecipitation and Western blotting experiments with cultured M phi or tissue extracts demonstrated that the Ag recognized by SER-4 mAb is a single chain molecule with an apparent Mr by SDS-PAGE of 185 x 10(3) (reduced) or 170 x 10(3) (non- reduced) and is distinct from members of the leukocyte common Ag family. Expression of SER and SER-4 Ag in culture were closely correlated and depended on the presence of mouse serum for optimal induction. Further evidence that the SER-4 Ag is functionally equivalent to SER was provided by immunocytochemistry in which the overall pattern of staining in tissues was consistent with previous rosetting experiments. In the bone marrow, expression of the SER-4 Ag was restricted to the resident bone marrow M phi population with no expression on monocytes. High expression was also observed on stromal M phi within the subcapsular sinus and medullary cords in lymph nodes and on marginal metallophils in the spleen. These results therefore confirm that SER is a novel M phi-restricted receptor whose distribution and properties indicate a role in cellular interactions in hematopoietic and lymphoid tissues.
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McMenamin, P. G (1999). Dendritic cells and macrophages in the uveal tract of the normal mouse eye. Br J Ophthalmol 83: 598-604 [Abstract] [Full Text]
Barnes, Y. C., Skelton, T. P., Stamenkovic, I., Sgroi, D. C. (1999). Sialylation of the Sialic Acid Binding Lectin Sialoadhesin Regulates Its Ability to Mediate Cell Adhesion. Blood 93: 1245-1252 [Abstract] [Full Text]
Scott, C.L., Hughes, D.A., Cary, D., Nicola, N.A., Begley, C.G., Robb, L. (1998). Functional Analysis of Mature Hematopoietic Cells From Mice Lacking the beta c Chain of the Granulocyte-Macrophage Colony-Stimulating Factor Receptor. Blood 92: 4119-4127 [Abstract] [Full Text]
Cornish, A. L., Freeman, S., Forbes, G., Ni, J., Zhang, M., Cepeda, M., Gentz, R., Augustus, M., Carter, K. C., Crocker, P. R. (1998). Characterization of Siglec-5, a Novel Glycoprotein Expressed on Myeloid Cells Related to CD33. Blood 92: 2123-2132 [Abstract] [Full Text]
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Pron, B., Boumaila, C., Jaubert, F., Sarnacki, S., Monnet, J.-P., Berche, P., Gaillard, J.-L. (1998). Comprehensive Study of the Intestinal Stage of Listeriosis in a Rat Ligated Ileal Loop System. Infect. Immun. 66: 747-755 [Abstract] [Full Text]
Inaba, K., Pack, M., Inaba, M., Sakuta, H., Isdell, F., Steinman, R. M. (1997). High Levels of a Major Histocompatibility Complex II-Self Peptide Complex on Dendritic Cells from the T Cell Areas of Lymph Nodes. JEM 186: 665-672 [Abstract] [Full Text]
Collins, B. E., Kiso, M., Hasegawa, A., Tropak, M. B., Roder, J. C., Crocker, P. R., Schnaar, R. L. (1997). Binding Specificities of the Sialoadhesin Family of I-type Lectins. SIALIC ACID LINKAGE AND SUBSTRUCTURE REQUIREMENTS FOR BINDING OF MYELIN-ASSOCIATED GLYCOPROTEIN, SCHWANN CELL MYELIN PROTEIN, AND SIALOADHESIN. J. Biol. Chem. 272: 16889-16895 [Abstract] [Full Text]
Barbe, E, Huitinga, I, Dopp, E., Bauer, J, Dijkstra, C. (1996). A novel bone marrow frozen section assay for studying hematopoietic interactions in situ: the role of stromal bone marrow macrophages in erythroblast binding. J. Cell Sci. 109: 2937-2945 [Abstract]
Nath, D., van der Merwe, P. A., Kelm, S�r., Bradfield, P., Crocker, P. R. (1995). The Amino-terminal Immunoglobulin-like Domain of Sialoadhesin Contains the Sialic Acid Binding Site. J. Biol. Chem. 270: 26184-26191 [Abstract] [Full Text]
Powell, L. D., Varki, A. (1995). I-type Lectins. J. Biol. Chem. 270: 14243-14246 [Full Text]
Witmer-Pack, M., Crowley, M., Inaba, K, Steinman, R. (1993). Macrophages, but not dendritic cells, accumulate colloidal carbon following administration in situ. J. Cell Sci. 105: 965-973 [Abstract]
Witmer-Pack, M., Hughes, D., Schuler, G, Lawson, L, McWilliam, A, Inaba, K, Steinman, R., Gordon, S (1993). Identification of macrophages and dendritic cells in the osteopetrotic (op/op) mouse. J. Cell Sci. 104: 1021-1029 [Abstract]