A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes

doi:10.1084/jem.169.1.73

This Article

	Full Text (PDF, 1023K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hunig, T.
	Articles by Tiefenthaler, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hunig, T.
	Articles by Tiefenthaler, G.


Social Bookmarking

	What's this?

ARTICLES

A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes

T Hunig, HJ Wallny, JK Hartley, A Lawetzky and G Tiefenthaler
Genzentrum der Ludwig Maximilians Universitat, Munchen, Federal Republic of Germany.

mAb R73 detects a T cell-specific surface molecule consisting of two disulfide-linked subunits of 40 and 46 kD, respectively, on 97% of peripheral rat T cells, as defined by the OX-52 marker. Of the few OX- 52+ R73- cells, none are CD4+ but many express the CD8 antigen known to be present on rat NK cells. mAb R73 is mitogenic for unseparated spleen cells and for purified T cells. In the absence of non-T "accessory cells", stimulation by R73 requires artificial crosslinking of the mAb and is largely dependent on exogenous IL-2. Overnight incubation of purified T cells with crosslinked R73 mAb induces blastoid transformation, IL-2-R expression, and modulation of the R73 antigen. In the rat thymus, mature medullary cells express the R73 determinant at the same level as peripheral T cells, whereas 94% of CD4-CD8- thymocytes are R73-. The major CD4+8+ thymocyte population contains 25% R73- and 70% R73low cells. Thymocytes of the CD4-CD8+OX-44- subpopulation that are the direct precursors of CD4+CD8+ cells display a continuum of R73 antigen density from undetectable to very low levels. We conclude that R73 is most likely directed at a constant determinant of the rat alpha/beta heterodimeric TCR and suggest that CD8+ immature thymocytes are the first cells in the T cell differentiation pathway to express this molecule at their surface.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Reijerkerk, A., Kooij, G., van der Pol, S. M. A., Leyen, T., van het Hof, B., Couraud, P.-O., Vivien, D., Dijkstra, C. D., de Vries, H. E. (2008). Tissue-Type Plasminogen Activator Is a Regulator of Monocyte Diapedesis through the Brain Endothelial Barrier. J. Immunol. 181: 3567-3574 [Abstract] [Full Text]
Castro, M. A. A., Oliveira, M. I., Nunes, R. J., Fabre, S., Barbosa, R., Peixoto, A., Brown, M. H., Parnes, J. R., Bismuth, G., Moreira, A., Rocha, B., Carmo, A. M. (2007). Extracellular Isoforms of CD6 Generated by Alternative Splicing Regulate Targeting of CD6 to the Immunological Synapse. J. Immunol. 178: 4351-4361 [Abstract] [Full Text]
Dennehy, K. M., Elias, F., Na, S.-Y., Fischer, K.-D., Hunig, T., Luhder, F. (2007). Mitogenic CD28 Signals Require the Exchange Factor Vav1 to Enhance TCR Signaling at the SLP-76-Vav-Itk Signalosome. J. Immunol. 178: 1363-1371 [Abstract] [Full Text]
Schreibelt, G., Musters, R. J. P., Reijerkerk, A., de Groot, L. R., van der Pol, S. M. A., Hendrikx, E. M. L., Dopp, E. D., Dijkstra, C. D., Drukarch, B., de Vries, H. E. (2006). Lipoic Acid Affects Cellular Migration into the Central Nervous System and Stabilizes Blood-Brain Barrier Integrity. J. Immunol. 177: 2630-2637 [Abstract] [Full Text]
Spargo, L. D., Cleland, L. G, Cockshell, M. P, Mayrhofer, G. (2006). Recruitment and proliferation of CD4+ T cells in synovium following adoptive transfer of adjuvant-induced arthritis. Int Immunol 18: 897-910 [Abstract] [Full Text]
Dennehy, K. M., Elias, F., Zeder-Lutz, G., Ding, X., Altschuh, D., Luhder, F., Hunig, T. (2006). Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses. J. Immunol. 176: 5725-5729 [Abstract] [Full Text]
Holmberg, J., Tuncel, J., Yamada, H., Lu, S., Olofsson, P., Holmdahl, R. (2006). Pristane, a Non-Antigenic Adjuvant, Induces MHC Class II-Restricted, Arthritogenic T Cells in the Rat. J. Immunol. 176: 1172-1179 [Abstract] [Full Text]
Dion, C., Carter, C., Hepburn, L., Coadwell, W. J., Morgan, G., Graham, M., Pugh, N., Anderson, G., Butcher, G. W., Miller, J. R. (2005). Expression of the Ian family of putative GTPases during T cell development and description of an Ian with three sets of GTP/GDP-binding motifs. Int Immunol 17: 1257-1268 [Abstract] [Full Text]
van den Brandt, J., Kwon, S.-H., Hunig, T., McPherson, K. G., Reichardt, H. M. (2005). Sustained Pre-TCR Expression in Notch1IC-Transgenic Rats Impairs T Cell Maturation and Selection. J. Immunol. 174: 7845-7852 [Abstract] [Full Text]
Kreiss, M., Asmuss, A., Krejci, K., Lindemann, D., Miyoshi-Akiyama, T., Uchiyama, T., Rink, L., Broeren, C. P. M., Herrmann, T. (2004). Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (V{beta}8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol 16: 655-663 [Abstract] [Full Text]
Floris, S., Blezer, E. L. A., Schreibelt, G., Dopp, E., van der Pol, S. M. A., Schadee-Eestermans, I. L., Nicolay, K., Dijkstra, C. D., de Vries, H. E. (2004). Blood-brain barrier permeability and monocyte infiltration in experimental allergic encephalomyelitis: A quantitative MRI study. Brain 127: 616-627 [Abstract] [Full Text]
Westgaard, I. H., Dissen, E., Torgersen, K. M., Lazetic, S., Lanier, L. L., Phillips, J. H., Fossum, S. (2003). The Lectin-like Receptor KLRE1 Inhibits Natural Killer Cell Cytotoxicity. JEM 197: 1551-1561 [Abstract] [Full Text]
Dennehy, K. M., Kerstan, A., Bischof, A., Park, J.-H., Na, S.-Y., Hunig, T. (2003). Mitogenic signals through CD28 activate the protein kinase C{theta}-NF-{kappa}B pathway in primary peripheral T cells. Int Immunol 15: 655-663 [Abstract] [Full Text]
Luhder, F., Huang, Y., Dennehy, K. M., Guntermann, C., Muller, I., Winkler, E., Kerkau, T., Ikemizu, S., Davis, S. J., Hanke, T., Hunig, T. (2003). Topological Requirements and Signaling Properties of T Cell-activating, Anti-CD28 Antibody Superagonists. JEM 197: 955-966 [Abstract] [Full Text]
Duplan, V., Dutartre, P., Mars, L. T., Liblau, R. S., Druet, P., Saoudi, A. (2003). LF 15-0195 Inhibits the Development of Rat Central Nervous System Autoimmunity by Inducing Long-Lasting Tolerance in Autoreactive CD4 T Cells. J. Immunol. 170: 2179-2185 [Abstract] [Full Text]
Pelegri, C., Vilaplana, J., Castellote, C., Rabanal, M., Franch, A., Castell, M. (2003). Circadian rhythms in surface molecules of rat blood lymphocytes. Am. J. Physiol. Cell Physiol. 284: C67-C76 [Abstract] [Full Text]
Haddad, E.-B., Underwood, S. L., Dabrowski, D., Birrell, M. A., McCluskie, K., Battram, C. H., Pecoraro, M., Foster, M. L., Belvisi, M. G. (2002). Critical Role for T Cells in Sephadex-Induced Airway Inflammation: Pharmacological and Immunological Characterization and Molecular Biomarker Identification. J. Immunol. 168: 3004-3016 [Abstract] [Full Text]
Cautain, B., Damoiseaux, J., Bernard, I., Xystrakis, E., Fournie, E., van Breda Vriesman, P., Druet, P., Saoudi, A. (2002). The CD8 T Cell Compartment Plays a Dominant Role in the Deficiency of Brown-Norway Rats to Mount a Proper Type 1 Immune Response. J. Immunol. 168: 162-170 [Abstract] [Full Text]
Spicer, S. T., Ha, H., Boyd, R. A., He, X. Y., Carter, N., Tran, G., Penny, M. J., Hodgkinson, S. J., Hall, B. M. (2001). IL-4 Therapy Prevents the Development of Proteinuria in Active Heymann Nephritis by Inhibition of Tc1 Cells. J. Immunol. 167: 3725-3733 [Abstract] [Full Text]
Korn, T., Toyka, K., Hartung, H.-P., Jung, S. (2001). Suppression of experimental autoimmune neuritis by leflunomide. Brain 124: 1791-1802 [Abstract] [Full Text]
Bowman, L. M., Holt, P. G. (2001). Selective Enhancement of Systemic Th1 Immunity in Immunologically Immature Rats with an Orally Administered Bacterial Extract. Infect. Immun. 69: 3719-3727 [Abstract] [Full Text]
Hoffmann, J C, Peters, K, Henschke, S, Herrmann, B, Pfister, K, Westermann, J, Zeitz, M (2001). Role of T lymphocytes in rat 2,4,6-trinitrobenzene sulphonic acid (TNBS) induced colitis: increased mortality after {gamma}{delta} T cell depletion and no effect of {alpha}{beta} T cell depletion. Gut 48: 489-495 [Abstract] [Full Text]
Subra, J.-F., Cautain, B., Xystrakis, E., Mas, M., Lagrange, D., van der Heijden, H., van de Gaar, M.-J., Druet, P., Fournie, G. J., Saoudi, A., Damoiseaux, J. (2001). The Balance Between CD45RChigh and CD45RClow CD4 T Cells in Rats Is Intrinsic to Bone Marrow-Derived Cells and Is Genetically Controlled. J. Immunol. 166: 2944-2952 [Abstract] [Full Text]
Torres-Nagel, N., Mehling, B., LeRolle, A.-F., Joly, E., Hunig, T. (2001). Genetic control of peripheral TCRAV usage by representation in the preselection repertoire and MHC allele-specific overselection. Int Immunol 13: 63-73 [Abstract] [Full Text]
Moore, J. K., Scheinman, R. I., Bellgrau, D. (2001). The Identification of a Novel T Cell Activation State Controlled by a Diabetogenic Gene. J. Immunol. 166: 241-248 [Abstract] [Full Text]
Dammers, P. M., Visser, A., Popa, E. R., Nieuwenhuis, P., Kroese, F. G. M. (2000). Most Marginal Zone B Cells in Rat Express Germline Encoded Ig VH Genes and Are Ligand Selected. J. Immunol. 165: 6156-6169 [Abstract] [Full Text]
Kipnis, J., Yoles, E., Porat, Z., Cohen, A., Mor, F., Sela, M., Cohen, I. R., Schwartz, M. (2000). T cell immunity to copolymer 1 confers neuroprotection on the damaged optic nerve: Possible therapy for optic neuropathies. Proc. Natl. Acad. Sci. USA 97: 7446-7451 [Abstract] [Full Text]
Wiegers, G. J., Stec, I. E. M., Klinkert, W. E. F., Reul, J. M. H. M. (2000). Glucocorticoids Regulate TCR-Induced Elevation of CD4: Functional Implications. J. Immunol. 164: 6213-6220 [Abstract] [Full Text]
Matsumoto, Y., Jee, Y., Sugisaki, M. (2000). Successful TCR-Based Immunotherapy for Autoimmune Myocarditis with DNA Vaccines After Rapid Identification of Pathogenic TCR. J. Immunol. 164: 2248-2254 [Abstract] [Full Text]
Patel, D. M., Arnold, P. Y., White, G. A., Nardella, J. P., Mannie, M. D. (1999). Class II MHC/Peptide Complexes Are Released from APC and Are Acquired by T Cell Responders During Specific Antigen Recognition. J. Immunol. 163: 5201-5210 [Abstract] [Full Text]
Luettig, B., Pape, L., Bode, U., Bell, E. B., Sparshott, S. M., Wagner, S., Westermann, J. (1999). Naive and Memory T Lymphocytes Migrate in Comparable Numbers Through Normal Rat Liver: Activated T Cells Accumulate in the Periportal Field. J. Immunol. 163: 4300-4307 [Abstract] [Full Text]
Damoiseaux, J. G. M. C., Cautain, B., Bernard, I., Mas, M., van Breda Vriesman, P. J. C., Druet, P., Fournie, G., Saoudi, A. (1999). A Dominant Role for the Thymus and MHC Genes in Determining the Peripheral CD4/CD8 T Cell Ratio in the Rat. J. Immunol. 163: 2983-2989 [Abstract] [Full Text]
FOUCHER, P., HEERINGA, P., PETERSEN, A. H., HUITEMA, M. G., BROUWER, E., COHEN TERVAERT, J. W., PROP, J., CAMUS, P., WEENING, J. J., KALLENBERG, C. G. M. (1999). Antimyeloperoxidase-associated Lung Disease . An Experimental Model. Am. J. Respir. Crit. Care Med. 160: 987-994 [Abstract] [Full Text]
MOALEM, G., MONSONEGO, A., SHANI, Y., COHEN, I. R., SCHWARTZ, M. (1999). Differential T cell response in central and peripheral nerve injury: connection with immune privilege. FASEB J. 13: 1207-1217 [Abstract] [Full Text]
Ramanathan, S., Poussier, P. (1999). T Cell Reconstitution of BB/W Rats After the Initiation of Insulitis Precipitates the Onset of Diabetes. J. Immunol. 162: 5134-5142 [Abstract] [Full Text]
Stienekemeier, M., Herrmann, T., Kruse, N., Weishaupt, A., Weilbach, F. X., Giegerich, G., Theofilopoulos, A., Jung, S., Gold, R. (1999). Heterogeneity of T-cell receptor usage in experimental autoimmune neuritis in the Lewis rat. Brain 122: 523-535 [Abstract] [Full Text]
Herrmann, T., Hofmann, K., Torres Nagel, N. E., Asmu�, A., Hunig, T., Wonigeit, K. (1999). Differential CD4/CD8 subset-specific expression of highly homologous rat Tcrb-V8 family members suggests a role of CDR2 and/or CDR4 (HV4) in MHC class-specific thymic selection. Int Immunol 11: 435-444 [Abstract] [Full Text]
Falgarone, G., Blanchard, H. S., Virecoulon, F., Simonet, M., Breban, M. (1999). Coordinate Involvement of Invasin and Yop Proteins in a Yersinia pseudotuberculosis-Specific Class I-Restricted Cytotoxic T Cell-Mediated Response. J. Immunol. 162: 2875-2883 [Abstract] [Full Text]
Penny, M. J., Boyd, R. A., Hall, B. M. (1998). Permanent CD8+ T Cell Depletion Prevents Proteinuria in Active Heymann Nephritis. JEM 188: 1775-1784 [Abstract] [Full Text]
Ramanathan, S., Norwich, K., Poussier, P. (1998). Antigen Activation Rescues Recent Thymic Emigrants from Programmed Cell Death in the BB Rat. J. Immunol. 160: 5757-5764 [Abstract] [Full Text]
Sedgwick, J. D., Ford, A. L., Foulcher, E., Airriess, R. (1998). Central Nervous System Microglial Cell Activation and Proliferation Follows Direct Interaction with Tissue-Infiltrating T Cell Blasts. J. Immunol. 160: 5320-5330 [Abstract] [Full Text]
Beisser, P. S., Vink, C., Van Dam, J. G., Grauls, G., Vanherle, S. J.�V., Bruggeman, C. A. (1998). The R33 G Protein-Coupled Receptor Gene of Rat Cytomegalovirus Plays an Essential Role in the Pathogenesis of Viral Infection. J. Virol. 72: 2352-2363 [Abstract] [Full Text]
Mitnacht, R., Bischof, A., Torres-Nagel, N., Hunig, T. (1998). Opposite CD4/CD8 Lineage Decisions of CD4+8+ Mouse and Rat Thymocytes to Equivalent Triggering Signals: Correlation with Thymic Expression of a Truncated CD8{alpha} Chain in Mice But Not Rats. J. Immunol. 160: 700-707 [Abstract] [Full Text]
Bridoux, F., Badou, A., Saoudi, A., Bernard, I., Druet, E., Pasquier, R., Druet, P., Pelletier, L. (1997). Transforming Growth Factor {beta} (TGF-{beta})-dependent Inhibition of T Helper Cell 2 (Th2)-induced Autoimmunity by Self-Major Histocompatibility Complex (MHC) Class II-specific, Regulatory CD4+ T Cell Lines. JEM 185: 1769-1775 [Abstract] [Full Text]
Lan, H. Y., Bacher, M., Yang, N., Mu, W., Nikolic-Paterson, D. J., Metz, C., Meinhardt, A., Bucala, R., Atkins, R. C. (1997). The Pathogenic Role of Macrophage Migration Inhibitory Factor in Immunologically Induced Kidney Disease in the Rat. JEM 185: 1455-1466 [Abstract] [Full Text]
Jecker, P., Beuleke, A., Dressendorfer, I., Pabst, R., Westermann, J. (1997). Long-term Oral Application of 5-Bromo-2-Deoxyuridine Does Not Reliably Label Proliferating Immune Cells in the LEW Rat. J. Histochem. Cytochem. 45: 393-402 [Abstract] [Full Text]
Bunce, C., Bell, E. B. (1997). CD45RC Isoforms Define Two Types of CD4 Memory T Cells, One of which Depends on Persisting Antigen. JEM 185: 767-776 [Abstract] [Full Text]
McWilliam, A. S., Napoli, S., Marsh, A. M., Pemper, F. L., Nelson, D. J., Pimm, C. L., Stumbles, P. A., Wells, T. N.C., Holt, P. G. (1996). Dendritic Cells Are Recruited into the Airway Epithelium during the Inflammatory Response to a Broad Spectrum of Stimuli. JEM 184: 2429-2432 [Abstract] [Full Text]
Byrnes, A. P., MacLaren, R. E., Charlton, H. M. (1996). Immunological Instability of Persistent Adenovirus Vectors in the Brain: Peripheral Exposure to Vector Leads to Renewed Inflammation, Reduced Gene Expression, and Demyelination. J. Neurosci. 16: 3045-3055 [Abstract] [Full Text]
Posselt, A., Barker, C., Friedman, A., Naji, A (1992). Prevention of autoimmune diabetes in the BB rat by intrathymic islet transplantation at birth. Science 256: 1321-1324 [Abstract]
Hunig, T., Tiefenthaler, G., Lawetzky, A., Kubo, R., Schlipkoter, E. (1989). T-cell Subpopulations Expressing Distinct Forms of the TCR in Normal, Athymic, and Neonatally TCR {alpha}{beta}-suppressed Rats. Cold Spring Harb Symp Quant Biol 54: 61-68 [Abstract]