Journal of Experimental Medicine, Vol 169, 297-302, Copyright © 1989 by Rockefeller University Press

ARTICLES

Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen

G Chimini, P Pala, J Sire, BR Jordan and JL Maryanski
Centre d'Immunologie INSERM, Centre National de Recherche Scientifique de Marseille-Luminy, Marseille, France.

We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
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This article has been cited by other articles:

• Legge, K. L., Min, B., Potter, N. T., Zaghouani, H. (1997). Presentation of a T Cell Receptor Antagonist Peptide by Immunoglobulins Ablates Activation of T Cells by a Synthetic Peptide or Proteins Requiring Endocytic Processing. JEM 185: 1043-1054 [Abstract] [Full Text]  
• Zaghouani, H, Steinman, R, Nonacs, R, Shah, H, Gerhard, W, Bona, C (1993). Presentation of a viral T cell epitope expressed in the CDR3 region of a self immunoglobulin molecule. Science 259: 224-227 [Abstract]  

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