Both rat and mouse T cell receptors specific for the encephalitogenic determinant of myelin basic protein use similar V alpha and V beta chain genes even though the major histocompatibility complex and encephalitogenic determinants being recognized are different

doi:10.1084/jem.169.1.27

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Both rat and mouse T cell receptors specific for the encephalitogenic determinant of myelin basic protein use similar V alpha and V beta chain genes even though the major histocompatibility complex and encephalitogenic determinants being recognized are different

FR Burns, XB Li, N Shen, H Offner, YK Chou, AA Vandenbark and E Heber-Katz
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.

Prospects for specific immune intervention in T cell-mediated autoimmune disease via anti-idiotypic regulation depend on the degree of diversity of the responder cell antigen receptor repertoire. A highly heterogenous response against self epitopes offers little chance for such regulation. We report here that the Lewis rat autoimmune disease experimental allergic encephalomyelitis, generally considered to be a model of human multiple sclerosis, is caused by T cells that use a limited set of TCR V genes. We have cloned the rat TCR alpha and beta chain cDNAs from the Lewis rat x mouse T cell hybridoma 510, which retains the rat specificity for the encephalitogenic determinant of myelin basic protein (MBP). Using Northern blot analysis of T cell RNA with the cloned V region probes, we have found a specific, and near perfect, correlation between expression of TCR message hybridizing to the V alpha 510 and VB510 probes and specificity for the encephalitogenic determinant of MBP in both T cell hybridomas and encephalitogenic T cell clones. This restricted V gene usage provides a basis for observed idiotypic regulation of auto-reactive T cells, and possible therapy for autoimmune disease. A curious and unexplained observation is that the Lewis rat V alpha/V beta combination that dominates the encephalitogenic response to the 68-88 peptide of MBP is precisely the same V alpha/V beta combination used by the B10.PL mouse response to the encephalitogenic response to the 1-9 peptide of MBP.
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Heard, R N S, Teutsch, S M, Bennetts, B H, Lee, S D, Deane, E M, Stewart, G J (1999). Lack of restriction of T cell receptor beta variable gene usage in cerebrospinal fluid lymphocytes in acute optic neuritis. J. Neurol. Neurosurg. Psychiatry 67: 585-590 [Abstract] [Full Text]
Lenz, D. C., Wolf, N. A., Swanborg, R. H. (1999). Strain Variation in Autoimmunity: Attempted Tolerization of DA Rats Results in the Induction of Experimental Autoimmune Encephalomyelitis. J. Immunol. 163: 1763-1768 [Abstract] [Full Text]
Stienekemeier, M., Herrmann, T., Kruse, N., Weishaupt, A., Weilbach, F. X., Giegerich, G., Theofilopoulos, A., Jung, S., Gold, R. (1999). Heterogeneity of T-cell receptor usage in experimental autoimmune neuritis in the Lewis rat. Brain 122: 523-535 [Abstract] [Full Text]
Smeltz, R. B., Wauben, M. H. M., Wolf, N. A., Swanborg, R. H. (1999). Critical Requirement for Aspartic Acid at Position 82 of Myelin Basic Protein 73-86 for Recruitment of V{beta}8.2+ T Cells and Encephalitogenicity in the Lewis Rat. J. Immunol. 162: 829-836 [Abstract] [Full Text]
Buenafe, A. C., Offner, H., Machnicki, M., Elerding, H., Adlard, K., Jacobs, R., Vandenbark, A. A., Adamus, G. (1998). EAE TCR Motifs and Antigen Recognition in Myelin Basic Protein-Induced Anterior Uveitis in Lewis Rats. J. Immunol. 161: 2052-2059 [Abstract] [Full Text]
Hanawa, H., Inomata, T., Okura, Y., Hirono, S., Ogawa, Y., Izumi, T., Kodama, M., Aizawa, Y. (1998). T Cells With Similar T-Cell Receptor �-Chain Complementarity-Determining Region 3 Motifs Infiltrate Inflammatory Lesions of Synthetic Peptides Inducing Rat Autoimmune Myocarditis. Circ. Res. 83: 133-140 [Abstract] [Full Text]
Bourdette, D. N., Chou, Y. K., Whitham, R. H., Buckner, J., Kwon, H. J., Nepom, G. T., Buenafe, A., Cooper, S. A., Allegretta, M., Hashim, G. A., Offner, H., Vandenbark, A. A. (1998). Immunity to T Cell Receptor Peptides in Multiple Sclerosis. III. Preferential Immunogenicity of Complementarity-Determining Region 2 Peptides from Disease-Associated T Cell Receptor BV Genes. J. Immunol. 161: 1034-1044 [Abstract] [Full Text]
Weissert, R., Svenningsson, A., Lobell, A., de Graaf, K. L., Andersson, R., Olsson, T. (1998). Molecular and Genetic Requirements for Preferential Recruitment of TCRBV8S2+ T Cells in Lewis Rat Experimental Autoimmune Encephalomyelitis. J. Immunol. 160: 681-690 [Abstract] [Full Text]
Kim, G., Tanuma, N., Kojima, T., Kohyama, K., Suzuki, Y., Kawazoe, Y., Matsumoto, Y. (1998). CDR3 Size Spectratyping and Sequencing of Spectratype-Derived TCR of Spinal Cord T Cells in Autoimmune Encephalomyelitis. J. Immunol. 160: 509-513 [Abstract] [Full Text]
Martin, A., Nakashima, M., Zhou, A., Aronson, D., Werner, A. J., Davies, T. F. (1997). Detection of Major T Cell Epitopes on Human Thyroid Stimulating Hormone Receptor by Overriding Immune Heterogeneity in Patients with Graves' Disease. J. Clin. Endocrinol. Metab. 82: 3361-3366 [Abstract] [Full Text]
Kumar, V., Aziz, F., Sercarz, E., Miller, A. (1997). Regulatory T Cells Specific for the Same Framework 3 Region of the V{beta}8.2 Chain Are Involved in the Control of Collagen II-induced Arthritis and Experimental Autoimmune Encephalomyelitis. JEM 185: 1725-1733 [Abstract] [Full Text]
Offner, H, Hashim, G., Vandenbark, A. (1991). T cell receptor peptide therapy triggers autoregulation of experimental encephalomyelitis. Science 251: 430-432 [Abstract]
Sinha, A., Lopez, M., McDevitt, H. (1990). Autoimmune diseases: the failure of self tolerance. Science 248: 1380-1388 [Abstract]
Wucherpfennig, K., Ota, K, Endo, N, Seidman, J., Rosenzweig, A, Weiner, H., Hafler, D. (1990). Shared human T cell receptor V beta usage to immunodominant regions of myelin basic protein. Science 248: 1016-1019 [Abstract]
Allegretta, M, Nicklas, J., Sriram, S, Albertini, R. (1990). T cells responsive to myelin basic protein in patients with multiple sclerosis. Science 247: 718-721 [Abstract]
Howell, M., Winters, S., Olee, T, Powell, H., Carlo, D., Brostoff, S. (1989). Vaccination against experimental allergic encephalomyelitis with T cell receptor peptides. Science 246: 668-670 [Abstract]
Hood, L., Kumar, V., Osman, G., Beall, S.S., Gomez, C., Funkhouser, W., Kono, D.H., Nickerson, D., Zaller, D.M., Urban, J.L. (1989). Autoimmune Disease and T-cell Immunologic Recognition. Cold Spring Harb Symp Quant Biol 54: 859-874 [Abstract]
Heber-Katz, E. (1989). A New Hierarchy of TCR Specificity: Autoimmune Diseases Are Defined by Particular V{alpha}V{beta} Combinations and Not by Antigen Specificity. Cold Spring Harb Symp Quant Biol 54: 875-878 [Abstract]