Journal of Experimental Medicine, Vol 169, 115-133, Copyright © 1989 by Rockefeller University Press

ARTICLES

Molecular genetic analysis of 178 I-Abm12-reactive T cells

J Bill, J Yague, VB Appel, J White, G Horn, HA Erlich and E Palmer
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

We have studied the genetic diversity of the TCR repertoire to the murine alloantigen I-Abm12 by generating a panel of 178 C57BL/10- derived I-Abm12-reactive T cell hybridomas. The expression of V alpha and V beta gene families was examined in this panel and the frequency of expression of V beta, but not ofV alpha, gene families differed significantly from that observed in a companion panel of random C57BL/10-derived hybridomas. The V beta 5 gene family was expressed significantly less frequently while the V beta 14, V beta 15, and V beta 16 genes were expressed significantly more frequently in the panel of I-Abm12-reactive than in the panel of random hybridomas. The junctional regions (VJ alpha and VDJ beta) of TCR V alpha and V beta genes from selected I-Abm12-specific hybridomas were amplified using the polymerase chain reaction, and directly sequenced. Surprisingly, no conserved J alpha, D beta, J beta, or N region-encoded sequences among these selected I-Abm12-reactive TCRs were identified. Thus, the T cell response to an I-A alloantigen that differs by only three amino acid residues from the I-A molecule of the responding strain is genetically complex but nonrandom. We have estimated that the repertoire to this alloantigen is comprised of at least 37 different TCRs.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Schenk, S., Kish, D. D., He, C., El-Sawy, T., Chiffoleau, E., Chen, C., Wu, Z., Sandner, S., Gorbachev, A. V., Fukamachi, K., Heeger, P. S., Sayegh, M. H., Turka, L. A., Fairchild, R. L. (2005). Alloreactive T Cell Responses and Acute Rejection of Single Class II MHC-Disparate Heart Allografts Are under Strict Regulation by CD4+CD25+ T Cells. J. Immunol. 174: 3741-3748 [Abstract] [Full Text]  
• Kraj, P., Pacholczyk, R., Ignatowicz, L. (2001). {{alpha}}{{beta}}TCRs Differ in the Degree of Their Specificity for the Positively Selecting MHC/Peptide Ligand. J. Immunol. 166: 2251-2259 [Abstract] [Full Text]  
• Erlich, H., Gelfand, D, Sninsky, J. (1991). Recent advances in the polymerase chain reaction. Science 252: 1643-1651 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS