The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 168, 1767-1779, Copyright © 1988 by Rockefeller University Press

ARTICLES

A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I

DL Rosenstreich, JH Tu, PR Kinkade, I Maurer-Fogy, J Kahn, RW Barton and PR Farina
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.

We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
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