Journal of Experimental Medicine, Vol 168, 1339-1349, Copyright © 1988 by Rockefeller University Press

ARTICLES

Interaction and sequence diversity among T15 VH genes in CBA/J mice

SE Ferguson, S Rudikoff and BA Osborne
Program in Molecular and Cellular Biology, University of Massachusetts, Amherst 01003.

Nucleotide sequences of the four genes composing the T15 heavy chain variable region (VH) family of the CBA/J mouse have been determined. Comparison of these sequences with their published BALB/c and C57BL/10 homologues reveals that nucleotide differences found between given alleles of two strains, i.e., CBA/J and BALB/c, are observed in other family members of the same strain. We suggest that these patterns of sequence variation are most readily explained by gene interaction (conversion). Additionally, the sequence of a CBA/J hybridoma, 6G6, proposed to have been generated by gene conversion, is directly encoded by the CBA/J V11 gene indicating that the putative conversion has occurred meiotically in the germline. These results are consistent with the premise that gene correction is occurring frequently among members of this family and that such processes may contribute significantly to the evolution of Ig variable region genes even in the relatively short time frame of inbred strain derivation.
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This article has been cited by other articles:

• Whitcomb, E. A., Haines, B. B., Parmelee, A. P., Pearlman, A. M., Brodeur, P. H. (1999). Germline Structure and Differential Utilization of Igha and Ighb VH10 Genes. J. Immunol. 162: 1541-1550 [Abstract] [Full Text]  
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