Unusual phenotype of B cells in the thymus of normal mice

doi:10.1084/jem.168.2.811

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Unusual phenotype of B cells in the thymus of normal mice

M Miyama-Inaba, S Kuma, K Inaba, H Ogata, H Iwai, R Yasumizu, S Muramatsu, RM Steinman and S Ikehara
First Department of Pathology, Kansai Medical University, Moriguchi, Japan.

A small number of B cells are found in the thymus of normal mice. A population of B lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4, and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+ (CD5), Ia+, B220low (CD45R), and Mac-1+ (CD 11b). In contrast, splenic B cells lacked CD5 and CD11b and expressed higher levels of B220 and Ia antigens. These results indicate that most thymic B cells have the phenotype of the Ly-1 B cell subset, which was identified previously as a trace subpopulation in some peripheral tissues and is thought to play a role in autoantibody formation.
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