Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma

doi:10.1084/jem.167.4.1493

This Article

	Full Text (PDF, 416K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ballhausen, W. G.
	Articles by Mak, T. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ballhausen, W. G.
	Articles by Mak, T. W.


Social Bookmarking

	What's this?

ARTICLES

Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma

WG Ballhausen, AB Reske-Kunz, B Tourvieille, PS Ohashi, JR Parnes and TW Mak
Ontario Cancer Institute, Toronto, Canada.

We have transfected the mouse CD4 gene into a beef insulin (BI)- specific murine T helper hybridoma that lacks CD4 surface expression. The CD4-expressing transfectants have acquired an additional reactivity for pork insulin (PI), which was not detectable in the original recipient cell. The transfectants' response to PI can be completely abrogated by anti-CD4 antibodies. The transfected clone showed a 50- fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. These experiments suggest that CD4 may be important in determining the antigen fine specificity and, therefore, may also play a role in altering the T cell repertoire.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Chung, C. D., Patel, V. P., Moran, M., Lewis, L. A., Miceli, M. C. (2000). Galectin-1 Induces Partial TCR {zeta}-Chain Phosphorylation and Antagonizes Processive TCR Signal Transduction. J. Immunol. 165: 3722-3729 [Abstract] [Full Text]
Vidal, K., Daniel, C., Hill, M., Littman, D. R., Allen, P. M. (1999). Differential Requirements for CD4 in TCR-Ligand Interactions. J. Immunol. 163: 4811-4818 [Abstract] [Full Text]
Vespa, G. N. R., Lewis, L. A., Kozak, K. R., Moran, M., Nguyen, J. T., Baum, L. G., Miceli, M. C. (1999). Galectin-1 Specifically Modulates TCR Signals to Enhance TCR Apoptosis but Inhibit IL-2 Production and Proliferation. J. Immunol. 162: 799-806 [Abstract] [Full Text]
Davidson, D., Cloutier, J.-F., Gregorieff, A., Veillette, A. (1997). Inhibitory Tyrosine Protein Kinase p50csk Is Associated with Protein-tyrosine Phosphatase PTP-PEST in Hemopoietic and Non-hemopoietic Cells. J. Biol. Chem. 272: 23455-23462 [Abstract] [Full Text]
Weil, R., Cloutier, J.-F.�o., Fournel, M., Veillette, A. (1995). Regulation of Zap-70 by Src Family Tyrosine Protein Kinases in an Antigen-specific T-cell Line. J. Biol. Chem. 270: 2791-2799 [Abstract] [Full Text]
Parnes, J.R., von Hoegen, P., Miceli, M.C., Zamoyska, R. (1989). Role of CD4 and CD8 in Enhancing T-cell Responses to Antigen. Cold Spring Harb Symp Quant Biol 54: 649-655 [Abstract]