Role of hemolysin for the intracellular growth of Listeria monocytogenes

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Role of hemolysin for the intracellular growth of Listeria monocytogenes

DA Portnoy, PS Jacks and DJ Hinrichs
Department of Microbiology and Immunology, School of Medicine, Washington University, St. Louis, Missouri 63110.

Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.
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Nadon, C. A., Bowen, B. M., Wiedmann, M., Boor, K. J. (2002). Sigma B Contributes to PrfA-Mediated Virulence in Listeria monocytogenes. Infect. Immun. 70: 3948-3952 [Abstract] [Full Text]
Spory, A., Bosserhoff, A., von Rhein, C., Goebel, W., Ludwig, A. (2002). Differential Regulation of Multiple Proteins of Escherichia coli and Salmonella enterica Serovar Typhimurium by the Transcriptional Regulator SlyA. J. Bacteriol. 184: 3549-3559 [Abstract] [Full Text]
Dudani, R., Chapdelaine, Y., Faassen, H. v., Smith, D. K., Shen, H., Krishnan, L., Sad, S. (2002). Multiple Mechanisms Compensate to Enhance Tumor-Protective CD8+ T Cell Response in the Long-Term Despite Poor CD8+ T Cell Priming Initially: Comparison Between an Acute Versus a Chronic Intracellular Bacterium Expressing a Model Antigen. J. Immunol. 168: 5737-5745 [Abstract] [Full Text]
Dudani, R., Chapdelaine, Y., van Faassen, H., Smith, D. K., Shen, H., Krishnan, L., Sad, S. (2002). Preexisting Inflammation Due to Mycobacterium bovis BCG Infection Differentially Modulates T-Cell Priming against a Replicating or Nonreplicating Immunogen. Infect. Immun. 70: 1957-1964 [Abstract] [Full Text]
Glomski, I. J., Gedde, M. M., Tsang, A. W., Swanson, J. A., Portnoy, D. A. (2002). The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells. JCB 156: 1029-1038 [Abstract] [Full Text]
Nomura, T., Kawamura, I., Tsuchiya, K., Kohda, C., Baba, H., Ito, Y., Kimoto, T., Watanabe, I., Mitsuyama, M. (2002). Essential Role of Interleukin-12 (IL-12) and IL-18 for Gamma Interferon Production Induced by Listeriolysin O in Mouse Spleen Cells. Infect. Immun. 70: 1049-1055 [Abstract] [Full Text]
Shetron-Rama, L. M., Marquis, H., Bouwer, H. G. A., Freitag, N. E. (2002). Intracellular Induction of Listeria monocytogenes actA Expression. Infect. Immun. 70: 1087-1096 [Abstract] [Full Text]
Kohda, C., Kawamura, I., Baba, H., Nomura, T., Ito, Y., Kimoto, T., Watanabe, I., Mitsuyama, M. (2002). Dissociated Linkage of Cytokine-Inducing Activity and Cytotoxicity to Different Domains of Listeriolysin O from Listeria monocytogenes. Infect. Immun. 70: 1334-1341 [Abstract] [Full Text]
Hamilton, S. E., Tvinnereim, A. R., Harty, J. T. (2001). Listeria monocytogenes Infection Overcomes the Requirement for CD40 Ligand in Exogenous Antigen Presentation to CD8+ T Cells. J. Immunol. 167: 5603-5609 [Abstract] [Full Text]
Dubail, I., Autret, N., Beretti, J.-L., Kayal, S., Berche, P., Charbit, A. (2001). Functional assembly of two membrane-binding domains in listeriolysin O, the cytolysin of Listeria monocytogenes. Microbiology 147: 2679-2688 [Abstract] [Full Text]
Popov, I., Dela Cruz, C. S., Barber, B. H., Chiu, B., Inman, R. D. (2001). The Effect of an Anti-HLA-B27 Immune Response on CTL Recognition of Chlamydia. J. Immunol. 167: 3375-3382 [Abstract] [Full Text]
Wilson, R. L., Tvinnereim, A. R., Jones, B. D., Harty, J. T. (2001). Identification of Listeria monocytogenes In Vivo-Induced Genes by Fluorescence-Activated Cell Sorting. Infect. Immun. 69: 5016-5024 [Abstract] [Full Text]