Journal of Experimental Medicine, Vol 167, 1391-1405, Copyright © 1988 by Rockefeller University Press

ARTICLES

Synthetic peptides as antigens and competitors in recognition by H-2- restricted cytolytic T cells specific for HLA

JL Maryanski, P Pala, JC Cerottini and G Corradin
Ludwig Institute for Cancer Research, Lausanne Branch, Switzerland.

The specificity of peptide recognition by a number of Kd-restricted CTL clones specific for HLA-CW3 or HLA-A24 was investigated. The CTL clones were derived from DBA/2 (H-2d) mice immunized with syngeneic P815 mouse cells transfected with genes encoding HLA-CW3 or HLA-A24 class I molecules. We had previously shown that CTL clones that lysed P815-CW3 transfectant target cells could lyse P815 (HLA-) target cells incubated with synthetic CW3 peptides corresponding to the COOH-terminal end of the alpha 2 domain. In the present study, we found that Kd-restricted CTL clones that lysed P815-A24 transfectant target cells recognized a synthetic peptide from the same region (residues 170-182) of the A24 molecule. CW3 and A24 differ by only one amino acid within this region. Recognition of CW3 or A24 peptides corresponded exactly with lysis of P815-HLA transfectants both for clones that mutually exclusively lysed CW3 or A24 transfectant target cells and for CW3/A24 crossreactive CTL clones. The latter CTL clones that lysed both CW3 and A24 transfectant target cells showed a clear preference for the peptide corresponding to the immunizing HLA allele. The homologous CW3 and A24 peptides could compete with each other for recognition, in contrast to a peptide from the same region of HLA-B7. Peptides from the corresponding region of the endogenous Kd and Dd/Ld molecules could also inhibit recognition of CW3 and A24 peptides. Competition with peptides apparently occurred at the level of the target cell. These results are consistent with a model whereby MHC class I molecules position protein fragments or peptides for specific recognition by T cells.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Mo, A. X. Y., van Lelyveld, S. F. L., Craiu, A., Rock, K. L. (2000). Sequences That Flank Subdominant and Cryptic Epitopes Influence the Proteolytic Generation of MHC Class I-Presented Peptides. J. Immunol. 164: 4003-4010 [Abstract] [Full Text]  
• Takahashi, H, Merli, S, Putney, S., Houghten, R, Moss, B, Germain, R., Berzofsky, J. (1989). A single amino acid interchange yields reciprocal CTL specificities for HIV-1 gp160. Science 246: 118-121 [Abstract]  
• Townsend, A., Ohlen, C., Foster, L., Bastin, J., Ljunggren, H.-G., Karre, K. (1989). A Mutant Cell in Which Association of Class I Heavy and Light Chains Is Induced by Viral Peptides. Cold Spring Harb Symp Quant Biol 54: 299-308 [Abstract]  
• Maryanski, J.L., Abastado, J.-P., Corradin, G., Cerottini, J.-C. (1989). Structural Features of Peptides Recognized by H-2Kd-restricted T Cells. Cold Spring Harb Symp Quant Biol 54: 545-550 [Abstract]  
• Boon, T., Van Pel, A., De Plaen, E., Chomez, P., Lurquin, C., Szikora, J.-P, Sibille, C., Mariame, B., Van den Eynde, B., Lethe, B., Brichart, V. (1989). Genes Coding for T-cell-defined Tum Transplantation Antigens: Point Mutations, Antigenic Peptides, and Subgenic Expression. Cold Spring Harb Symp Quant Biol 54: 587-596 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS