Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1)

doi:10.1084/jem.167.4.1323

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Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1)

ML Dustin, KH Singer, DT Tuck and TA Springer
Laboratory of Membrane Immunochemistry, Dana Farber Cancer Institute, Boston, Massachusetts 02115.

The cell surface expression and function of the LFA-1 ligand, intercellular adhesion molecule 1 (ICAM-1), on epidermal keratinocytes (EK) was studied. ICAM-1 expression on the surface of cultured EK was either absent or weak, but was induced by treating EK with rIFN-gamma or TNF for 4-48 h. IFN-gamma and TNF were synergistic. IFN-gamma treatment increased T lymphoblast adhesion from less than 2% to 20-40%, with a concentration dependence similar to that seen for ICAM-1 induction. All of the adhesion to EK was inhibited by LFA-1 and ICAM-1 mAbs, but not by HLA-DR, CD2, or LFA-3 mAbs. There was no difference in the level of T lymphoblast adhesion to IFN-gamma-treated allogeneic or autologous EK. ICAM-1 purified from the HeLa epithelioid cell line and reconstituted into planar membranes also supported efficient adhesion of T lymphoblasts that was blocked by LFA-1 mAb bound to the T lymphoblasts or ICAM-1 mAb bound to the planar membranes. T lymphoblasts adherent to EK or ICAM-1 planar membranes were isolated by panning, and surface markers were analyzed by immunofluorescence flow cytometry. The adherent T cells were a phenotypically skewed subpopulation. They were enriched for CD8+ cells and expressed 1.5-2.5- fold higher LFA-1 and CD2 compared with the unseparated population.
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Wang, C.-C., Lin, W.-N., Lee, C.-W., Lin, C.-C., Luo, S.-F., Wang, J.-S., Yang, C.-M. (2005). Involvement of p42/p44 MAPK, p38 MAPK, JNK, and NF-{kappa}B in IL-1{beta}-induced VCAM-1 expression in human tracheal smooth muscle cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 288: L227-L237 [Abstract] [Full Text]
de Repentigny, L., Lewandowski, D., Jolicoeur, P. (2004). Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection. Clin. Microbiol. Rev. 17: 729-759 [Abstract] [Full Text]
Chan, G., Stinski, M.F., Guilbert, L.J. (2004). Human Cytomegalovirus-Induced Upregulation of Intercellular Cell Adhesion Molecule-1 on Villous Syncytiotrophoblasts. Biol. Reprod. 71: 797-803 [Abstract] [Full Text]
Krueger, G., Callis, K. (2004). Potential of Tumor Necrosis Factor Inhibitors in Psoriasis and Psoriatic Arthritis. Arch Dermatol 140: 218-225 [Abstract] [Full Text]
YAMADA, M., NAKAE, H., YUMOTO, H., SHINOHARA, C., EBISU, S., MATSUO, T. (2002). N-Acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (ICAM-1) production by human oral epithelial cells. J Med Microbiol 51: 1080-1089 [Abstract] [Full Text]
Gottlieb, A. B., Krueger, J. G., Wittkowski, K., Dedrick, R., Walicke, P. A., Garovoy, M. (2002). Psoriasis as a Model for T-Cell-Mediated Disease: Immunobiologic and Clinical Effects of Treatment With Multiple Doses of Efalizumab, an Anti-CD11a Antibody. Arch Dermatol 138: 591-600 [Abstract] [Full Text]
Chen, C.-C., Chen, J.-J., Chou, C.-Y. (2001). Protein Kinase Calpha but not p44/42 Mitogen-Activated Protein Kinase, p38, or c-Jun NH2-Terminal Kinase Is Required for Intercellular Adhesion Molecule-1 Expression Mediated by Interleukin-1beta : Involvement of Sequential Activation of Tyrosine Kinase, Nuclear Factor-kappa B-Inducing Kinase, and Ikappa B Kinase 2. Mol. Pharmacol. 58: 1479-1489 [Abstract] [Full Text]
Thomas, G., Speight, P.M. (2001). Cell Adhesion Molecules and Oral Cancer. CROBM 12: 479-498 [Abstract] [Full Text]
Fujisaki, T., Tanaka, Y., Fujii, K., Mine, S., Saito, K., Yamada, S., Yamashita, U., Irimura, T., Eto, S. (1999). CD44 Stimulation Induces Integrin-mediated Adhesion of Colon Cancer Cell Lines to Endothelial Cells by Up-Regulation of Integrins and c-Met and Activation of Integrins. Cancer Res. 59: 4427-4434 [Abstract] [Full Text]
Arnold, R., Seifert, M., Asadullah, K., Volk, H. D. (1999). Crosstalk Between Keratinocytes and T Lymphocytes via Fas/Fas Ligand Interaction: Modulation by Cytokines. J. Immunol. 162: 7140-7147 [Abstract] [Full Text]
Albanesi, C., Cavani, A., Girolomoni, G. (1999). IL-17 Is Produced by Nickel-Specific T Lymphocytes and Regulates ICAM-1 Expression and Chemokine Production in Human Keratinocytes: Synergistic or Antagonist Effects with IFN-{gamma} and TNF-{alpha}. J. Immunol. 162: 494-502 [Abstract] [Full Text]
Paxton, L. L.�L., Li, L.-J., Secor, V., Duff, J. L., Naik, S. M., Shibagaki, N., Caughman, S. W. (1997). Flanking Sequences for the Human Intercellular Adhesion Molecule-1 NF-kappa B Response Element Are Necessary for Tumor Necrosis Factor alpha -Induced Gene Expression. J. Biol. Chem. 272: 15928-15935 [Abstract] [Full Text]
Sais, G., Vidaller, A., Jucgla, A., Condom, E., Peyri, J. (1997). Adhesion Molecule Expression and Endothelial Cell Activation in Cutaneous Leukocytoclastic Vasculitis: An Immunohistologic and Clinical Study in 42 Patients. Arch Dermatol 133: 443-450 [Abstract]
Baranda, L., Torres-Alvarez, B., Cortes-Franco, R., Moncada, B., Portales-Perez, D. P., Gonzalez-Amaro, R. (1997). Involvement of Cell Adhesion and Activation Molecules in the Pathogenesis of Erythema Dyschromicum Perstans (Ashy Dermatitis) The Effect of Clofazimine Therapy. Arch Dermatol 133: 325-329 [Abstract]
Bennion, S., Norns, D. (1997). Ultraviolet light modulation of autoantigens, epidermal cytokines and adhesion molecules as contributing factors of the pathogenesis of cutaneous LE. Lupus 6: 181-192
Meijne, A., Driessens, M., La Riviere, G, Casey, D, Feltkamp, C., Roos, E (1994). LFA-1 integrin redistribution during T-cell hybridoma invasion of hepatocyte cultures and manganese-induced adhesion to ICAM-1. J. Cell Sci. 107: 2557-2566 [Abstract]
Crawford, J. M., Watanabe, K. (1994). Cell Adhesion Molecules in Inflammation and Immunity: Relevance to Periodontal Diseases. CROBM 5: 91-123 [Abstract] [Full Text]
Scher, R. L., Koch, W. M., Richtsmeier, W. J. (1993). Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma. Arch Otolaryngol Head Neck Surg 119: 432-438 [Abstract]
Kalish, R. S., Johnson, K. L., Hordinsky, M. K. (1992). Alopecia Areata: Autoreactive T Cells Are Variably Enriched in Scalp Lesions Relative to Peripheral Blood. Arch Dermatol 128: 1072-1077 [Abstract]
Kalish, R. S. (1991). Recent Developments in the Pathogenesis of Allergic Contact Dermatitis. Arch Dermatol 127: 1558-1563 [Abstract]
Strohal, R., Gillitzer, R., Zonzits, E., Stingl, G. (1991). Localized vs Generalized Pyogenic Granuloma: A Clinicopathologic Study. Arch Dermatol 127: 856-861 [Abstract]
Richtsmeier, W. J., Koch, W. M., McGuire, W. P., Poole, M. E., Chang, E. H. (1990). Phase I-II Study of Advanced Head and Neck Squamous Cell Carcinoma Patients Treated With Recombinant Human Interferon Gamma. Arch Otolaryngol Head Neck Surg 116: 1271-1277 [Abstract]
Wegner, C., Gundel, R., Reilly, P, Haynes, N, Letts, L., Rothlein, R (1990). Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science 247: 456-459 [Abstract]
Shiohara, T., Nickoloff, B. J., Sagawa, Y., Gomi, T., Nagashima, M. (1989). Fixed Drug Eruption: Expression of Epidermal Keratinocyte Intercellular Adhesion Molecule-1 (ICAM-1). Arch Dermatol 125: 1371-1376 [Abstract]
Kupper, T. S. (1989). Mechanisms of Cutaneous Inflammation: Interactions Between Epidermal Cytokines, Adhesion Molecules, and Leukocytes. Arch Dermatol 125: 1406-1412 [Abstract]
Nickoloff, B. J., Griffiths, C. E. M., Baadsgaard, O., Voorhees, J. J., Hanson, C. A., Cooper, K. D. (1989). Markedly Diminished Epidermal Keratinocyte Expression of Intercellular Adhesion Molecule-1 (ICAM-1) in Sezary Syndrome. JAMA 261: 2217-2221 [Abstract]
Nickoloff, B. J. (1988). Role of Interferon-{gamma} in Cutaneous Trafficking of Lymphocytes With Emphasis on Molecular and Cellular Adhesion Events. Arch Dermatol 124: 1835-1843 [Abstract]