Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells

doi:10.1084/jem.167.2.700

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Granulocyte/macrophage colony-stimulating factor and interleukin 1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells

C Heufler, F Koch and G Schuler
Department of Dermatology, University of Innsbruck, Austria.

Freshly isolated, murine epidermal Langerhans cells (LC) are weak accessory cells for primary T cell-dependent immune responses, but increase their stimulatory capacity at least 20-fold progressively over a 3-d culture with keratinocytes. We have studied the mediators of LC maturation. LC enriched from 12-h epidermal cultures by negative selection do not survive when cultured for 60 h in standard medium. LC survive and show increased stimulatory capacity for oxidative mitogenesis and the primary MLR when 30% keratinocyte-conditioned medium is included. Of the three cytokines that are known to be produced by keratinocytes, only granulocyte/macrophage CSF (GM-CSF) maintains viability and increases stimulatory capacity. IL-1 alone does not keep LC alive, but further enhances the stimulatory activity when combined with GM-CSF. IL-3 has no effect. The increase in LC stimulatory capacity is not due to increased Ia antigen expression, which does not change between 12 and 60 h. Function is not simply due to improved viability, as GM-CSF does not enhance the function of 12-h LC when added to the short-term oxidative mitogenesis assay. By generating LC with strong stimulating activity for resting T cells, GM- CSF and IL-1 may be critical in the sensitization phase of T cell- mediated immunity.
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Kodaira, Y., Nair, S. K., Wrenshall, L. E., Gilboa, E., Platt, J. L. (2000). Phenotypic and Functional Maturation of Dendritic Cells Mediated by Heparan Sulfate. J. Immunol. 165: 1599-1604 [Abstract] [Full Text]
Egeler, R. M., Favara, B. E., van Meurs, M., Laman, J. D., Claassen, E. (1999). Differential In Situ Cytokine Profiles of Langerhans-Like Cells and T Cells in Langerhans Cell Histiocytosis: Abundant Expression of Cytokines Relevant to Disease and Treatment. Blood 94: 4195-4201 [Abstract] [Full Text]
Fensterle, J., Grode, L., Hess, J., Kaufmann, S. H. E. (1999). Effective DNA Vaccination Against Listeriosis by Prime/Boost Inoculation with the Gene Gun. J. Immunol. 163: 4510-4518 [Abstract] [Full Text]
Avigan, D., Wu, Z., Gong, J., Joyce, R., Levine, J., Elias, A., Richardson, P., Milano, J., Kennedy, L., Anderson, K., Kufe, D. (1999). Selective in Vivo Mobilization with Granulocyte Macrophage Colony-stimulating Factor (GM-CSF)/Granulocyte-CSF ascompared to G-CSF Alone of Dendritic Cell Progenitors from Peripheral Blood Progenitor Cells in Patients with Advanced Breast Cancer Undergoing Autologous Transplantation. Clin. Cancer Res. 5: 2735-2741 [Abstract] [Full Text]
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Anjuere, F., Martin, P., Ferrero, I., Fraga, M. L., del Hoyo, G. M., Wright, N., Ardavin, C. (1999). Definition of Dendritic Cell Subpopulations Present in the Spleen, Peyer's Patches, Lymph Nodes, and Skin of the Mouse. Blood 93: 590-598 [Abstract] [Full Text]
Labeur, M. S., Roters, B., Pers, B., Mehling, A., Luger, T. A., Schwarz, T., Grabbe, S. (1999). Generation of Tumor Immunity by Bone Marrow-Derived Dendritic Cells Correlates with Dendritic Cell Maturation Stage. J. Immunol. 162: 168-175 [Abstract] [Full Text]
Cohen, Adam. D., Boyer, J. D., Weiner, D. B. (1998). Modulating the immune response to genetic immunization. FASEB J. 12: 1611-1626 [Abstract] [Full Text]
Kocikova, A., Kolesaric, A., Koszik, F., Stingl, G., Elbe-Burger, A. (1998). Murine Langerhans Cells Cultured Under Serum-Free Conditions Mature into Potent Stimulators of Primary Immune Responses In Vitro and In Vivo. J. Immunol. 161: 4033-4041 [Abstract] [Full Text]
Hurwitz, A. A., Yu, T. F.-Y., Leach, D. R., Allison, J. P. (1998). CTLA-4 blockade synergizes with tumor-derived granulocyte- macrophage colony-stimulating factor for treatment of an experimental mammary carcinoma. Proc. Natl. Acad. Sci. USA 95: 10067-10071 [Abstract] [Full Text]
Dieu, M.-C., Vanbervliet, B., Vicari, A., Bridon, J.-M., Oldham, E., Ait-Yahia, S., Briere, F., Zlotnik, A., Lebecque, S., Caux, C. (1998). Selective Recruitment of Immature and Mature Dendritic Cells by Distinct Chemokines Expressed in Different Anatomic Sites. JEM 188: 373-386 [Abstract] [Full Text]
Kraft, S., We{beta}endorf, J. H. M., Hanau, D., Bieber, T. (1998). Regulation of the High Affinity Receptor for IgE on Human Epidermal Langerhans Cells. J. Immunol. 161: 1000-1006 [Abstract] [Full Text]
Ross, R., Ross, X.-L., Schwing, J., Langin, T., Reske-Kunz, A. B. (1998). The Actin-Bundling Protein Fascin Is Involved in the Formation of Dendritic Processes in Maturing Epidermal Langerhans Cells. J. Immunol. 160: 3776-3782 [Abstract] [Full Text]
Rovere, P., Zimmermann, V. S., Forquet, F., Demandolx, D., Trucy, J., Ricciardi-Castagnoli, P., Davoust, J. (1998). Dendritic cell maturation and antigen presentation in the absence of�invariant�chain. Proc. Natl. Acad. Sci. USA 95: 1067-1072 [Abstract] [Full Text]
Kuhn, U., Brand, P., Willemsen, J., Jonuleit, H., Enk, A. H., van Brandwijk-Petershans, R., Saloga, J., Knop, J., Becker, D. (1998). Induction of Tyrosine Phosphorylation in Human MHC Class II-Positive Antigen-Presenting Cells by Stimulation with Contact Sensitizers. J. Immunol. 160: 667-673 [Abstract] [Full Text]
Nagoshi, M., Goedegebuure, P. S., Burger, U. L., Sadanaga, N., Chang, M. P., Eberlein, T. J. (1998). Successful Adoptive Cellular Immunotherapy Is Dependent on Induction of a Host Immune Response Triggered by Cytokine (IFN-{gamma} and Granulocyte/Macrophage Colony-Stimulating Factor) Producing Donor Tumor-Infiltrating Lymphocytes. J. Immunol. 160: 334-344 [Abstract] [Full Text]
Price, A. A., Cumberbatch, M., Kimber, I., Ager, A. (1997). {alpha}6 Integrins Are Required for Langerhans Cell Migration from the Epidermis. JEM 186: 1725-1735 [Abstract] [Full Text]
Weiss, J. M., Sleeman, J., Renkl, A. C., Dittmar, H., Termeer, C. C., Taxis, S., Howells, N., Hofmann, M., Kohler, G., Schopf, E., Ponta, H., Herrlich, P., Simon, J. C. (1997). An Essential Role for CD44 Variant Isoforms in Epidermal Langerhans Cell and Blood Dendritic Cell Function. JCB 137: 1137-1147 [Abstract] [Full Text]
Gaspari, A. A., Zalka, A. D., Payne, D., Menegus, M., Bunce, L. A., Abboud, C. N., Tyring, S. K. (1997). Successful Treatment of a Generalized Human Papillomavirus Infection With Granulocyte-Macrophage Colony-Stimulating Factor and Interferon Gamma Immunotherapy in a Patient With a Primary Immunodeficiency and Cyclic Neutropenia. Arch Dermatol 133: 491-496 [Abstract]
Winzler, C., Rovere, P., Rescigno, M., Granucci, F., Penna, G., Adorini, L., Zimmermann, V. S., Davoust, J., Ricciardi-Castagnoli, P. (1997). Maturation Stages of Mouse Dendritic Cells in Growth Factor-dependent Long-Term Cultures. JEM 185: 317-328 [Abstract] [Full Text]
Witmer-Pack, M., Hughes, D., Schuler, G, Lawson, L, McWilliam, A, Inaba, K, Steinman, R., Gordon, S (1993). Identification of macrophages and dendritic cells in the osteopetrotic (op/op) mouse. J. Cell Sci. 104: 1021-1029 [Abstract]
Horn, T. D., Burke, P. J., Karp, J. E., Hood, A. F. (1991). Intravenous Administration of Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor Causes a Cutaneous Eruption. Arch Dermatol 127: 49-52 [Abstract]