Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors

doi:10.1084/jem.166.5.1377

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Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors

NR Payne and MA Horwitz
Department of Pediatrics, UCLA School of Medicine 90024.

We have examined receptors mediating phagocytosis of the intracellular bacterial pathogen, Legionella pneumophila. Three mAbs against the type 3 complement receptor (CR3), which recognizes C3bi, inhibit adherence of L. pneumophila to monocytes by 64 +/- 8% to 74 +/- 11%. An mAb against the type 1 complement receptor (CR1), which recognizes C3b, inhibits adherence by 68 +/- 1%. mAbs against other monocyte surface antigens do not significantly influence adherence. Monocytes plated on substrates of L. pneumophila membranes modulate their CR1 and CR3 receptors but not Fc receptors; such monocytes bind 70% fewer C3b- coated erythrocytes and 53% fewer C3bi-coated erythrocytes than control monocytes. Adherence of L. pneumophila to monocytes in nonimmune sera is dependent on heat-labile serum opsonins; adherence is markedly reduced in heat-inactivated serum (84% reduction) or buffer alone (97% reduction) compared with fresh serum. mAbs against CR1 and CR3 receptors also inhibit L. pneumophila intracellular multiplication and protect monocyte monolayers from destruction by this bacterium. This study demonstrates that human monocyte complement receptors, CR1 and CR3, mediate phagocytosis of L. pneumophila. These receptors may play a general role in mediating phagocytosis of intracellular pathogens.
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Yoshizawa, S., Tateda, K., Matsumoto, T., Gondaira, F., Miyazaki, S., Standiford, T. J., Yamaguchi, K. (2005). Legionella pneumophila Evades Gamma Interferon-Mediated Growth Suppression through Interleukin-10 Induction in Bone Marrow-Derived Macrophages. Infect. Immun. 73: 2709-2717 [Abstract] [Full Text]
Molmeret, M., Horn, M., Wagner, M., Santic, M., Abu Kwaik, Y. (2005). Amoebae as Training Grounds for Intracellular Bacterial Pathogens. Appl. Environ. Microbiol. 71: 20-28 [Full Text]
Fajardo, M., Schleicher, M., Noegel, A., Bozzaro, S., Killinger, S., Heuner, K., Hacker, J., Steinert, M. (2004). Calnexin, calreticulin and cytoskeleton-associated proteins modulate uptake and growth of Legionella pneumophila in Dictyostelium discoideum. Microbiology 150: 2825-2835 [Abstract] [Full Text]
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Fields, B. S., Benson, R. F., Besser, R. E. (2002). Legionella and Legionnaires' Disease: 25 Years of Investigation. Clin. Microbiol. Rev. 15: 506-526 [Abstract] [Full Text]
Watarai, M., Derre, I., Kirby, J., Growney, J. D., Dietrich, W. F., Isberg, R. R. (2001). Legionella pneumophila Is Internalized by a Macropinocytotic Uptake Pathway Controlled by the Dot/Icm System and the Mouse Lgn1 Locus. JEM 194: 1081-1096 [Abstract] [Full Text]
Khelef, N., Shuman, H. A., Maxfield, F. R. (2001). Phagocytosis of Wild-Type Legionella pneumophila Occurs through a Wortmannin-Insensitive Pathway. Infect. Immun. 69: 5157-5161 [Abstract] [Full Text]
Tateda, K., Moore, T. A., Deng, J. C., Newstead, M. W., Zeng, X., Matsukawa, A., Swanson, M. S., Yamaguchi, K., Standiford, T. J. (2001). Early Recruitment of Neutrophils Determines Subsequent T1/T2 Host Responses in a Murine Model of Legionella pneumophila Pneumonia. J. Immunol. 166: 3355-3361 [Abstract] [Full Text]
Cirillo, S. L. G., Bermudez, L. E., El-Etr, S. H., Duhamel, G. E., Cirillo, J. D. (2001). Legionella pneumophila Entry Gene rtxA Is Involved in Virulence. Infect. Immun. 69: 508-517 [Abstract] [Full Text]
Bandyopadhyay, P., Steinman, H. M. (2000). Catalase-Peroxidases of Legionella pneumophila: Cloning of the katA Gene and Studies of KatA Function. J. Bacteriol. 182: 6679-6686 [Abstract] [Full Text]
Le Cabec, V., Cols, C., Maridonneau-Parini, I. (2000). Nonopsonic Phagocytosis of Zymosan and Mycobacterium kansasii by CR3 (CD11b/CD18) Involves Distinct Molecular Determinants and Is or Is Not Coupled with NADPH Oxidase Activation. Infect. Immun. 68: 4736-4745 [Abstract] [Full Text]
Herron, M. J., Nelson, C. M., Larson, J., Snapp, K. R., Kansas, G. S., Goodman, J. L. (2000). Intracellular Parasitism by the Human Granulocytic Ehrlichiosis Bacterium Through the P-Selectin Ligand, PSGL-1. Science 288: 1653-1656 [Abstract] [Full Text]
Cirillo, S. L. G., Lum, J., Cirillo, J. D. (2000). Identification of novel loci involved in entry by Legionella pneumophila. Microbiology 146: 1345-1359 [Abstract] [Full Text]
Eze, M. O., Yuan, L., Crawford, R. M., Paranavitana, C. M., Hadfield, T. L., Bhattacharjee, A. K., Warren, R. L., Hoover, D. L. (2000). Effects of Opsonization and Gamma Interferon on Growth of Brucella melitensis 16M in Mouse Peritoneal Macrophages In Vitro. Infect. Immun. 68: 257-263 [Abstract] [Full Text]
Cirillo, J. D., Cirillo, S. L. G., Yan, L., Bermudez, L. E., Falkow, S., Tompkins, L. S. (1999). Intracellular Growth in Acanthamoeba castellanii Affects Monocyte Entry Mechanisms and Enhances Virulence of Legionella pneumophila. Infect. Immun. 67: 4427-4434 [Abstract] [Full Text]
Hales, L. M., Shuman, H. A. (1999). The Legionella pneumophila rpoS Gene Is Required for Growth within Acanthamoeba castellanii. J. Bacteriol. 181: 4879-4889 [Abstract] [Full Text]
Luneberg, E., Zahringer, U., Knirel, Y. A., Steinmann, D., Hartmann, M., Steinmetz, I., Rohde, M., Kohl, J., Frosch, M. (1998). Phase-variable Expression of Lipopolysaccharide Contributes to the Virulence of Legionella pneumophila. JEM 188: 49-60 [Abstract] [Full Text]
Coxon, P. Y., Summersgill, J. T., Ramirez, J. A., Miller, R. D. (1998). Signal Transduction during Legionella pneumophila Entry into Human Monocytes. Infect. Immun. 66: 2905-2913 [Abstract] [Full Text]
Gao, L.-Y., Harb, O. S., Kwaik, Y. A. (1998). Identification of Macrophage-Specific Infectivity Loci (mil) of Legionella pneumophila That Are Not Required for Infectivity of Protozoa. Infect. Immun. 66: 883-892 [Abstract] [Full Text]
Rittig, M. G., Jagoda, J. C., Wilske, B., Murgia, R., Cinco, M., Repp, R., Burmester, G. R., Krause, A. (1998). Coiling Phagocytosis Discriminates between Different Spirochetes and Is Enhanced by Phorbol Myristate Acetate and Granulocyte- Macrophage Colony-Stimulating Factor. Infect. Immun. 66: 627-635 [Abstract] [Full Text]
Kwaik, Y. A. (1998). Induced Expression of the Legionella pneumophila Gene Encoding a 20-Kilodalton Protein during Intracellular Infection. Infect. Immun. 66: 203-212 [Abstract] [Full Text]
Venkataraman, C., Haack, B. J., Bondada, S., Kwaik, Y. A. (1997). Identification of a Gal/GalNAc Lectin in the Protozoan Hartmannella vermiformis as a Potential Receptor for Attachment and Invasion by the Legionnaires' Disease Bacterium. JEM 186: 537-547 [Abstract] [Full Text]
Isberg, R. (1991). Discrimination between intracellular uptake and surface adhesion of bacterial pathogens. Science 252: 934-938 [Abstract]