Journal of Experimental Medicine, Vol 166, 1192-1197, Copyright © 1987 by Rockefeller University Press

ARTICLES

A novel subset of human lymphocytes with a T cell receptor-gamma complex

S Jitsukawa, F Faure, M Lipinski, F Triebel and T Hercend
Laboratoire d'Immunologie Cellulaire, Departement de Biologie Clinique, Villejuif, France.

We have previously characterized a CD3+ T cell receptor (TCR) alpha/beta- human fetal cloned cell line, termed F6C7, which surface- expresses a CD3-associated gamma chain identified by anti-NKFi, an mAb with a restricted clonotypic reactivity. Here, we have produced an additional antibody, anti-Ti-gamma A, which recognizes a public epitope of the gamma molecule defined by anti-NKFi. Ti-gamma A is present on approximately 3% of circulating lymphocytes with a wide range (1-15%) among 30 healthy individuals tested. Two-color immunofluorescence experiments performed with anti-Ti-gamma A and BMA 031 mAb (a reagent specific for the TCR-alpha/beta receptor) showed that surface expression of Ti-alpha/beta and Ti-gamma A is mutually exclusive. Moreover, it was found that most Ti-gamma A+ cells are CD2+, CD3+, CD4- , CD5+, NKH1-, HLA class II-negative. In contrast, the expression of the CD8 molecule on these T lymphocytes appears to be variable from one individual to another. Finally, we found that Ti-gamma A+ cells represent a majority of peripheral lymphocytes that express CD3 proteins but not the TCR-alpha/beta heterodimer. The delineation of this unique lymphocyte subset should help further studies on the biology of cells with a CD3-associated gamma complex.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Wang, H., Lee, H. K., Bukowski, J. F., Li, H., Mariuzza, R. A., Chen, Z. W., Nam, K.-H., Morita, C. T. (2003). Conservation of Nonpeptide Antigen Recognition by Rhesus Monkey V{gamma}2V{delta}2 T Cells. J. Immunol. 170: 3696-3706 [Abstract] [Full Text]  
• Spada, F. M., Grant, E. P., Peters, P. J., Sugita, M., Melian, A., Leslie, D. S., Lee, H. K., van Donselaar, E., Hanson, D. A., Krensky, A. M., Majdic, O., Porcelli, S. A., Morita, C. T., Brenner, M. B. (2000). Self-Recognition of Cd1 by {gamma}/{delta} T Cells: Implications for Innate Immunity. JEM 191: 937-948 [Abstract] [Full Text]  
• Davodeau, F, Peyrat, M., Houde, I, Hallet, M., De Libero, G, Vie, H, Bonneville, M (1993). Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science 260: 1800-1802 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS