Fine mapping of epitopes by intradomain Kd/Dd recombinants

doi:10.1084/jem.166.2.327

This Article

	Full Text (PDF, 881K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Abastado, J. P.
	Articles by Kourilsky, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Abastado, J. P.
	Articles by Kourilsky, P.


Social Bookmarking

	What's this?

ARTICLES

Fine mapping of epitopes by intradomain Kd/Dd recombinants

JP Abastado, C Jaulin, MP Schutze, P Langlade-Demoyen, F Plata, K Ozato and P Kourilsky

11 intradomain recombinants between H-2Kd and H-2Dd were produced using an original technique based on in vivo recombination in Escherichia coli. After transfection into mouse L cells, all these recombinants were expressed at high levels on the cell surface. The specificities of 77 mAbs were examined on these cell lines. mAbs could be organized in 12 groups. In each group, a small number of amino acids participating in the recognized epitope(s) were identified. In a few instances, noncontinuous epitopes comprising amino acids belonging to different domains of the antigen were found. The data thus obtained are compatible with those produced in previous exon-shuffling experiments, but permit a much more precise definition of recognized epitope(s).
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Guil, S., Rodriguez-Castro, M., Aguilar, F., Villasevil, E. M., Anton, L. C., Del Val, M. (2006). Need for Tripeptidyl-peptidase II in Major Histocompatibility Complex Class I Viral Antigen Processing when Proteasomes are Detrimental. J. Biol. Chem. 281: 39925-39934 [Abstract] [Full Text]
Samino, Y., Lopez, D., Guil, S., Saveanu, L., van Endert, P. M., Del Val, M. (2006). A Long N-terminal-extended Nested Set of Abundant and Antigenic Major Histocompatibility Complex Class I Natural Ligands from HIV Envelope Protein. J. Biol. Chem. 281: 6358-6365 [Abstract] [Full Text]
Samino, Y., Lopez, D., Guil, S., de Leon, P., Del Val, M. (2004). An Endogenous HIV Envelope-derived Peptide without the Terminal NH3+ Group Anchor Is Physiologically Presented by Major Histocompatibility Complex Class I Molecules. J. Biol. Chem. 279: 1151-1160 [Abstract] [Full Text]
Fujimoto, C., Nakagawa, Y., Ohara, K., Takahashi, H. (2004). Polyriboinosinic polyribocytidylic acid [poly(I:C)]/TLR3 signaling allows class I processing of exogenous protein and induction of HIV-specific CD8+ cytotoxic T lymphocytes. Int Immunol 16: 55-63 [Abstract] [Full Text]
Matsumoto, N., Tajima, K., Mitsuki, M., Yamamoto, K. (2001). H-2 allele specificity of the NK cell C-type lectin-like MHC class I receptor Ly49A visualized by soluble Ly49A tetramer. Int Immunol 13: 615-623 [Abstract] [Full Text]
Matsumoto, N., Yokoyama, W. M., Kojima, S., Yamamoto, K. (2001). The NK Cell MHC Class I Receptor Ly49A Detects Mutations on H-2Dd Inside and Outside of the Peptide Binding Groove. J. Immunol. 166: 4422-4428 [Abstract] [Full Text]
Chung, D. H., Natarajan, K., Boyd, L. F., Tormo, J., Mariuzza, R. A., Yokoyama, W. M., Margulies, D. H. (2000). Mapping the Ligand of the NK Inhibitory Receptor Ly49A on Living Cells. J. Immunol. 165: 6922-6932 [Abstract] [Full Text]
Nakamura, M. C., Hayashi, S., Niemi, E. C., Ryan, J. C., Seaman, W. E. (2000). Activating Ly-49d and Inhibitory Ly-49a Natural Killer Cell Receptors Demonstrate Distinct Requirements for Interaction with H2-Dd. JEM 192: 447-454 [Abstract] [Full Text]
Sundback, J., Nakamura, M. C., Waldenstrom, M., Niemi, E. C., Seaman, W. E., Ryan, J. C., Karre, K. (1998). The {alpha}2 Domain of H-2Dd Restricts the Allelic Specificity of the Murine NK Cell Inhibitory Receptor Ly-49A. J. Immunol. 160: 5971-5978 [Abstract] [Full Text]
Cox, J., Yewdell, J., Eisenlohr, L., Johnson, P., Bennink, J. (1990). Antigen presentation requires transport of MHC class I molecules from the endoplasmic reticulum. Science 247: 715-718 [Abstract]
Maryanski, J.L., Abastado, J.-P., Corradin, G., Cerottini, J.-C. (1989). Structural Features of Peptides Recognized by H-2Kd-restricted T Cells. Cold Spring Harb Symp Quant Biol 54: 545-550 [Abstract]