Early genetic events in T cell development analyzed by in situ hybridization

doi:10.1084/jem.165.6.1624

This Article

	Full Text (PDF, 861K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pardoll, D. M.
	Articles by Schwartz, R. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pardoll, D. M.
	Articles by Schwartz, R. H.


Social Bookmarking

	What's this?

ARTICLES

Early genetic events in T cell development analyzed by in situ hybridization

DM Pardoll, BJ Fowlkes, RI Lechler, RN Germain and RH Schwartz

In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Syken, J., Shatz, C. J. (2003). Expression of T cell receptor {beta}locus in central nervous system neurons. Proc. Natl. Acad. Sci. USA 100: 13048-13053 [Abstract] [Full Text]
Zhao, F.-Q., Sheng, Z.-M., Tsai, M. M., Hubbs, A. E., Wang, R., O'Leary, T. J., Izon, D. J., Taubenberger, J. K. (2002). Serial analysis of gene expression in murine fetal thymocyte cell lines. Int Immunol 14: 1383-1395 [Abstract] [Full Text]
Hirayama, F., Aiba, Y., Ikebuchi, K., Sekiguchi, S., Ogawa, M. (1999). Differentiation in Culture of Murine Primitive Lymphohematopoietic Progenitors Toward T-Cell Lineage. Blood 93: 4187-4195 [Abstract] [Full Text]
Angelin-Duclos, C., Calame, K. (1998). Evidence that Immunoglobulin VH-DJ Recombination Does Not Require Germ Line Transcription of the Recombining Variable Gene Segment. Mol. Cell. Biol. 18: 6253-6264 [Abstract] [Full Text]
Oosterwegel, M, van de Wetering, M, Timmerman, J, Kruisbeek, A, Destree, O, Meijlink, F, Clevers, H (1993). Differential expression of the HMG box factors TCF-1 and LEF-1 during murine embryogenesis. Development 118: 439-448 [Abstract]
Takahama, Y, Singer, A (1992). Post-transcriptional regulation of early T cell development by T cell receptor signals. Science 258: 1456-1462 [Abstract]