Alloantigen persistence in induction and maintenance of transplantation tolerance

doi:10.1084/jem.165.6.1468

This Article

	Full Text (PDF, 837K)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JEM
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Morecki, S.
	Articles by Slavin, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Morecki, S.
	Articles by Slavin, S.


Social Bookmarking

	What's this?

ARTICLES

Alloantigen persistence in induction and maintenance of transplantation tolerance

S Morecki, B Leshem, A Eid and S Slavin

Infusion of parental bone marrow cells into F1 hybrids conditioned by total lymphoid irradiation (TLI) results in chimeras with a high percentage of donor-type cells, and without clinical signs of graft-vs.- host reaction. In these chimeras, a state of tolerance has been shown to be associated with paucity of cytotoxic T lymphocyte percursors (pCTL) reactive with host-type alloantigens. To determine whether the presence of tolerizing alloantigens is essential for maintenance of unresponsiveness, lymphohematopoietic cells obtained from such tolerant chimeras were transferred into supralethally irradiated recipients of two different genotypes: in one case the adoptive recipients were syngeneic with host-type cells, and in the other they were syngeneic with donor-type cells of the original chimeras, thus providing the chimeric cells with a tolerogen-free environment. After "parking" for 4 d in syngeneic donor-type mice, the transferred cells displayed a marked increase in the frequency of pCTL directed against tolerizing alloantigens, whereas a low pCTL frequency directed against the same H- 2 target cells was maintained in allogeneic tolerizing-type adoptive recipients. Multiple injections of adoptive donor-type mice with tolerizing-type cells of the original chimera reestablished a low level of cytotoxic precursors. Cytotoxic activity against unrelated alloantigens was independent of the presence of tolerogen-presenting cells in the adoptively transferred mice. Our experimental model suggests that persistence of cells bearing tolerizing alloantigens is an essential requirement for maintenance of previously established tolerance.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Kurtz, J., Shaffer, J., Lie, A., Anosova, N., Benichou, G., Sykes, M. (2004). Mechanisms of early peripheral CD4 T-cell tolerance induction by anti-CD154 monoclonal antibody and allogeneic bone marrow transplantation: evidence for anergy and deletion but not regulatory cells. Blood 103: 4336-4343 [Abstract] [Full Text]
Yamamoto, Y., Wang, B., Fukuhara, S., Ikehara, S., Good, R. A. (2002). Mixed peripheral blood stem cell transplantation for autoimmune disease in BXSB/MpJ mice. Blood 100: 1886-1893 [Abstract] [Full Text]
Garza, K. M., Agersborg, S. S., Baker, E., Tung, K. S. K. (2000). Persistence of Physiological Self Antigen Is Required for the Regulation of Self Tolerance. J. Immunol. 164: 3982-3989 [Abstract] [Full Text]