Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion

doi:10.1084/jem.165.3.677

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Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion

ML Dustin, ME Sanders, S Shaw and TA Springer

CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA- 3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, and this binding was inhibited by CD2 mAb. Conversely, purified LFA-3 inhibited binding of CD2 mAb to cells, and the concentration required for this effect suggests that LFA-3 half-saturated CD2 at 1-5 nM LFA-3. Purified LFA-3 inhibited rosetting of human and sheep erythrocytes with CD2+ T lymphoma cells and T lymphocytes, and mediated aggregation of a CD2+ T lymphoma cell line. Purified LFA-3 reconstituted into planar membranes mediated efficient CD2-dependent adhesion of T lymphoblasts. These data demonstrate that LFA-3 is a ligand for CD2 and that LFA-3 can mediate T lymphocyte adhesion.
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Fournel, S., Robinet, E., Bonnefoy-Berard, N., Assossou, O., Flacher, M., Waldmann, H., Bismuth, G., Revillard, J.-P. (1998). A Noncomitogenic CD2R Monoclonal Antibody Induces Apoptosis of Activated T Cells by a CD95/CD95-L-Dependent Pathway. J. Immunol. 160: 4313-4321 [Abstract] [Full Text]
Itzhaky, D., Raz, N., Hollander, N. (1998). The Glycosylphosphatidylinositol-Anchored Form and the Transmembrane Form of CD58 Associate with Protein Kinases. J. Immunol. 160: 4361-4366 [Abstract] [Full Text]
Dumont, C., Deas, O., Mollereau, B., Hebib, C., Giovino-Barry, V., Bernard, A., Hirsch, F., Charpentier, B., Senik, A. (1998). Potent Apoptotic Signaling and Subsequent Unresponsiveness Induced by a Single CD2 mAb (BTI-322) in Activated Human Peripheral T Cells. J. Immunol. 160: 3797-3804 [Abstract] [Full Text]
Dustin, M. L., Golan, D. E., Zhu, D.-M., Miller, J. M., Meier, W., Davies, E. A., van der Merwe, P. A. (1997). Low Affinity Interaction of Human or Rat T Cell Adhesion Molecule CD2 with Its Ligand Aligns Adhering Membranes to Achieve High Physiological Affinity. J. Biol. Chem. 272: 30889-30898 [Abstract] [Full Text]
Dustin, M. L. (1997). Adhesive Bond Dynamics in Contacts between T Lymphocytes and Glass-supported Planar Bilayers Reconstituted with the Immunoglobulin-related Adhesion Molecule CD58. J. Biol. Chem. 272: 15782-15788 [Abstract] [Full Text]
Teh, S.-J., Killeen, N., Tarakhovsky, A., Littman, D. R., Teh, H.-S. (1997). CD2 Regulates the Positive Selection and Function of Antigen-Specific CD4-CD8+ T Cells. Blood 89: 1308-1318 [Abstract] [Full Text]
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Reinherz, E.L., Chang, H.-C., Clayton, L.K., Gardner, P., Howard, F.D., Koyasu, S., Jin, Y.-J., Moingeon, P., Sayre, P.H. (1989). The Biology of Human CD2. Cold Spring Harb Symp Quant Biol 54: 611-625 [Abstract]