The Journal of Experimental Medicine
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Journal of Experimental Medicine, Vol 165, 578-583, Copyright © 1987 by Rockefeller University Press

ARTICLES

A murine hybridoma with large cytoplasmic inclusions of kappa light chains

DC Reason

A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
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